Two examples of null alleles at the D19S433 locus due to the same 4 bp deletion in the presumptive primer binding site of the AmpFlSTR Identifiler kit

Two cases of allelic loss at the D19S433 locus after multiplex PCR with the AmpFlSTR Identifiler kit (Applied Biosystems) are described. In both cases the failure of PCR resulted in genetic inconsistencies due to opposite homozygosity. After singleplex PCR with published primers additional alleles were observed and Mendelian inheritance was restored. These PCR products were sequenced and in both cases the same 4 bp deletion near the 3′ end of the repeat region was detected in two alleles of different length. The frequency of these null alleles (two events in 1026 allelic transfers) amounts to 0.0019 (95% confidence limits: 0.0002-0.0070).     

卡网技术与运营分析

本文列举了当前应用 IC卡中存在的问题 ,首次提出卡网的概念 ,并初步设计出卡网运营的模式     

基于Web2.0的网络思想政治教育研究

Web2.0是相对于以web1.0为代表的传统媒体的新一代互联网应用的统称.随着Web2.0的发展,网络内容的生产方式、传播方式、文化产品等出现了许多新情况、新特点.在认识web2.0的技术特征、社会文化特征的基础上,研究怎么样利用web2.0更好地发挥互联网的思想政治教育功能,最大限度地遏制其负面影响,是思想政治教育学必须承担的理论任务.     

新媒体技术条件下的整合性营销传播策略

web2.0的兴起引发了传播形态、媒体环境及营销手段等种种变化,这些均为整合营销传播的实施提供了新的契机。目前,我们要充分发挥web2.0的应用元素,实现营销与传播的融合,以web2.0为中心全面整合营销手段及传播渠道,通过话题引导建立与利益攸关者的良好关系,推进整合营销传播的执行。     

大鼠液压冲击脑损伤bFGF及其受体FGFR1 mRNA表达

目的 观察脑损伤后 bFGF及其受体 FGFRl mRNA表达及其时序性变化,探讨脑损伤的分子机制及法医学脑损伤时间推断。方法 用原位杂交(ISH)和RT-PCR技术观察大鼠液压冲击脑损伤后bFGF及FGFRl mRNA在伤后不同时间(30min、1h、3h、6h、12h、1d、3d、7d)的表达情况,以正常大鼠及手术大鼠作为对照。结果(1)正常及手术对照组大鼠脑组织内有低水平的bFGF及FGFRl mRNA表达;(2)脑损伤后6h～3d,大脑皮质和脑干bFGF/FGFRl mRNA水平逐渐增加,7d时已有所下降,但仍未恢复到基础水平;(3)海马冲击后3h即开始显著增加,3d开始下降,7d时已恢复到基础水平。结论 脑损伤可诱导bFGF/FGFRl基因表达,机体对脑损伤存在自身保护机制,其表达的时序性改变可望用于法医学脑损伤时间推断。     

《开放英语》（4）教学模式的探索

远程开放教育必须积极探索适合成人的英语教学模式。根据远程开放教育的课程及教学对象的特点,构建网络环境下导学、助学、促学三结合的教学模式,对于增强学生的自主学习能力,体现“以学生为中心”的现代教学理念,都是一种有益的尝试。     

农村宅基地使用权流转再探——兼谈小产权房合法化问题

小产权房合法化问题的核心是农村宅基地使用权流转的限制问题。我国现行法律实际上并没有禁止农村宅基地使用权的转让。从理论角度和现状考察分析,农民对其拥有的宅基地使用权都应该有权自由流转,因此小产权房合法化有其合理依据。我国应完善相关立法并逐步放开宅基地使用权流转限制,还农民的主人地位,使农民真正拥有宅基地房屋的支配权。     

BrowStExPlus: A Tool to Aggregate IndexedDB Artifacts for Forensic Analysis

As the Internet and World Wide Web have rapidly evolved and revolutionized the applications in everyday life, it is a demanding challenge for investigators to keep up with the emerging technologies for forensic analyses. Investigating web browser usages for criminal activities, also known as web browser forensics, is a significant part of digital forensics as crucial browsing information of the suspect can be discovered. Particularly, in this study, an emerging web storage technology, called IndexedDB, is examined. Characteristics of IndexedDB technology in five major web browsers under three major operating systems are scrutinized. Also, top 15 US websites ranked by Alexa are investigated for their data storage in IndexedDB. User screen names, ids, and records of conversations, permissions, and image locations are some of the data found in IndexedDB. Furthermore, BrowStEx, a proof‐of‐concept tool previously developed, is extended and cultivated into BrowStExPlus, with which aggregating IndexedDB artifacts is demonstrated.     

试论工程监理对首都建设工程施工现场的环境管理

从北京市建设工程的实际情况出发,从法规、标准、技术、经济等层面,对工程监理在施工现场实施环境监督管理,特别是施工扬尘控制监督的举措进行了探讨。结果表明,该举措意义重大、技术经济可行,但是尚需要在法规、标准方面得到进一步的支持。