非目的片段对mtDNA测序影响的实验观察

目的通过重新设计引物,去除非目的片段对mtDNA测序的影响。方法在目的片段原引物内侧重设计引物,用该引物替代原引物进行测序,其测序图谱与原图谱进行对比。结果重新设计引物后,获得了清晰的序列图谱。结论通过重设计引物消除了非目的片断对mtDNA测序的影响。     

骆驼斯氏副柔线虫病传播媒介的筛选与确定

对从内蒙古骆驼生活环境中采集到的24种蝇进行普通生物学剖检,并进一步用斯氏副柔线虫特异性引物ST1、ST2对蝇体内检获的幼虫进行分子生物学鉴定,以筛选与确定骆驼斯氏副柔线虫病的传播媒介。结果显示,在西方角蝇和截脉角蝇体内均发现了疑似斯氏副柔线虫幼虫,感染强度为1～22条/个,雌蝇与雄蝇感染强度差异不显著;幼虫长度为1.033～1.934mm;雄蝇的感染率为43.8%～48.0%,雌蝇的感染率为35.7%～46.2%。扩增得到的部分ITS序列(包括5.8S)与GenBank中登录的斯氏副柔线虫ITS序列同源性高达99.3%。基本确定了西方角蝇和截脉角蝇为斯氏副柔线虫病的传播媒介。     

《源氏物语》与《蜻蛉日记》之间关系探微

《源氏物语》与《蜻蛉日记》两部文学作品之间关系密切,《源氏物语》是《蜻蛉日记》的继承、扬弃与超越。本论从两位女性作家的执笔动机、文论观念、女性意识3个方面推考两部著作的内在联系。两部作品中的若干相同与相似重申了被男性主流文学掠过边缘经典女流文学的价值,正是这一边缘经典文学更为真实记录了当时社会现状、女性思想、男女的情感生活,两位女性作家的叙事才情更是勿容忽视。     

1型鸭肝炎病毒E53株和HP-1株全基因组序列分析

根据已发表的1型鸭肝炎病毒(DHV-1)基因组序列设计并合成引物,通过RT-PCR方法对DHV-1 E53株和HP-1株的基因组序列进行分段扩增并测序.BLAST分析确定为DHV-1型基因组序列,GenBank登录号分别为EF151313和EF151312.E53株与已发表的参考毒株的核苷酸同源性为94.6%～99.4%;HP-1株与已发表的参考毒株的核苷酸同源性为94.4%～99.6%.系统进化树分析结果表明,E53株与北京BJ株进化关系最近;HP-1株与R85952株进化关系最近.     

Two Walks: Palestine and the Peak District. A Conversation between Raja Shehadeh and Marina Lewycka, September 2008

In the summer of 2008 novelist Marina Lewycka and author and human rights lawyer Raja Shehadeh went on two walks: the first in Palestine near Ramallah near the Beit Eil Jewish settlement, the second on Kinder Scout in the Peak District, the site of the mass trespass in 1932 to reclaim the right to walk on the hills. This account of the conversation they recorded on the second walk includes observations by Marina on writing about Palestine and Israel and reflections by Raja about the effect of the Israeli occupation on the fragile Palestinian landscape. It also includes their discussions on writing, walking and the meaning it has to each of them, as well as political comments on the Israeli-Palestinian conflict evoked by visiting these sites. They wonder whether a similar act of resistance would be possible in Palestine and what it would mean to the future of the conflict.     

‘The Message Is in the Book’: What Virago's Sale in 1995 Means for Feminist Publishing

This article examines the UK publisher Virago, the world's largest women's imprint and the best known of the second-wave feminist publishing houses that sprang up during the 1970s and 1980s. Feminist publishing remains a peculiarly unacknowledged and underexamined aspect of feminist history, but one that had real influence and effect. This article gives a historical account of Virago, noting its intention to enact feminist politicking through the act of publishing books. The author looks at the effect Virago had on the industry more widely, on literary culture and on attitudes to women. The author also examines changing formulations of feminism and how these were reflected—or not—through Virago's published output. The author then moves on to her central proposition: that Virago's sale in 1995, rather than marking the death of feminist publishing (as was stated in media comment at the time), was in fact the point at which it was saved. Virago's move into Little, Brown, coinciding with the rise of an increasingly commodified consumer culture, the conglomeration of the book industry and more pluralized expressions of feminism, allowed it to continue to work as a publisher of women's writing while none of its contemporaries survived. The author looks in detail at the changes post-1995 within the book industry, within feminism and in wider culture, as well as at Virago. The author asks whether Virago still has value as a ‘feminist’ imprint and how it has sought to remain vital and relevant.     

谈法学毕业论文的写作

在高等院校法律教学过程中,法学毕业论文的写作和指导具有重要的地位,是检验学员学习法律水平和质量的重要尺度和考核手段,也是衡量学生是否具有从事法律工作必备的理论素养的一个标志。但是,因为法学教育的大环境逐渐变化和社会的复杂化与浮躁,导致法学论文写作质量的下降。本文针对近年来指导学员论文写作的常见问题和不足,提出若干参考性意见,供大家参考。     

旋毛虫半胱氨酸蛋白酶抑制剂基因TsCystatin1的克隆及序列分析

为了研究旋毛虫半胱氨酸蛋白酶抑制剂的功能,应用电子克隆的方法从GenBank EST数据库获得1个旋毛虫半胱氨酸蛋白酶抑制剂基因(TsCystatin1)的部分cDNA序列,据此设计引物,并以旋毛虫新生幼虫总RNA为模板,进行反转录及PCR,克隆到该基因的完整开放阅读框序列,利用生物信息学软件对其进行了预测分析。结果表明,TsCystatin1 cDNA序列含有一个由666个核苷酸组成的开放阅读框架,编码由221个氨基酸残基组成的多肽,蛋白分子质量理论值为24.3ku,理论等电点为4.44。TsCystatin1第1～25位氨基酸残基为信号肽序列,具有半胱氨酸蛋白酶抑制剂的保守序列QVVAG,其C末端具有3处N-糖基化位点以及4个链内二硫键所需的半胱氨酸残基。该蛋白的二级结构中α螺旋占18.6%,β折叠占23.5%,其余57.9%为转角。结构域分析表明,该蛋白具有一个半胱氨酸蛋白酶抑制剂样结构域,属于半胱氨酸蛋白酶抑制剂家族2,与其他线虫半胱氨酸蛋白酶抑制剂的同源性较高。     

犬瘟热病毒TN野毒株核蛋白基因的克隆与序列分析

根据GenBank中收录的犬瘟热病毒(CDV)Onderstepoort株核蛋白基因(N)序列,设计了1对特异性引物,用该引物对分离的TN野毒株进行了RT PCR扩增;将扩增得到的PCR片段纯化后与pGEM T质粒连接,得到重组质粒pTN。经核苷酸序列测定,CDVTN株N基因的ORF全长1569bp,编码523个氨基酸;将TN野毒株与GenBank中收录的其他CDV毒株进行比较,N基因核苷酸序列的同源性为94%～99%,推导的N蛋白氨基酸序列的同源性为97%～99%。TN株与Onderstepoort疫苗株氨基酸序列的差异主要集中在N端(17～159位)和C端(408～519位),中间的区域则高度保守。此外,在CDVN蛋白N端281～289位上也发现存在与麻疹病毒(MV)N蛋白完全相同的Y P A L G L H E F9肽序列,推测可能是诱导CTL活性的靶蛋白的抗原表位之一。氨基酸组成分析发现,推导的N蛋白氨基酸序列中亮氨酸、丝氨酸和异亮氨酸所占比例很高,提示该蛋白具有高度螺旋和缠绕的分子结构。     

特殊文件相对形成时间的鉴定

伪造、变造、异常处理文书的书写时间鉴定在诉讼中的作用越来越重要。笔者在多年的文书鉴定工作中曾遇到多起特殊处理的文件,如人为老化文件、塑封文件,污染文件以及书写于传真纸、宣纸等特定材料上的文件,在对这些文件的检验过程中,笔者进行了部分研究,获得一些实际体会。