二参真武汤调控PI3K/AKT/mTOR信号通路抑制心肌纤维化的体外实验研究

目的 探究二参真武汤抑制心肌细胞纤维化的作用机制。方法 采用灌胃给予SPF大鼠二参真武汤、贝那普利7 d的方法制备含药血清,从出生3 d内的乳鼠心脏中提取原代心肌成纤维细胞,采用免疫荧光和台盼蓝染色法鉴定细胞纯度与成活率,采用CCK-8法确定二参真武汤含药血清的最佳给药浓度与给药时间,采用血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)刺激原代心肌成纤维细胞复制心肌纤维化模型,将原代心肌成纤维细胞分为正常组、模型组、二参真武汤组、贝那普利组,采用ELISA法检测胶原蛋白(collagenase, Col)-Ⅰ和Col-Ⅲ含量,采用Western blot法检测平滑肌肌动蛋白(alpha-smooth muscle actin, α-SMA)、Col-Ⅰ、Col-Ⅲ、PI3K、p-PI3K、AKT、p-AKT、mTOR、p-mTOR蛋白表达水平,qRT-PCR法检测α-SMA、Col-Ⅰ、Col-Ⅲ、PI3K、AKT、mTOR mRNA表达水平。结果 提取的原代心肌成纤维细胞纯度与成活率均达到95%以上。与正常组比较,模型组、Col-Ⅰ、Col-Ⅲ含量显著升高(P<0.05),α...     

Portable high‐resolution automated 3D imaging for footwear and tire impression capture

The forensic science community raised the need for improved evidence recognition, collection, and visualization analytical instrumentation for field and laboratory use. While the 3D optical techniques for imaging static objects have been extensively studied, there is still a major gap between current knowledge and collecting high‐quality footwear and tire impression evidence. Among optical means for 3D imaging, digital fringe projection (DFP) techniques reconstruct 3D shape from phase information, achieving camera‐pixel spatial resolution. This paper presents a high‐resolution 3D imaging technology using DFP techniques dedicated to footwear and tire impression capture. We developed fully automated software algorithms and a graphical user interface (GUI) that allow anyone without training to operate for high‐quality 3D data capture. We performed accuracy evaluations and comparisons comparing with the commercial high‐end 3D scanner and carried out qualitative tests for various impressions comparing with the current practices. Overall, our technology achieves similar levels of accuracy and resolution with a high‐end commercially available 3D scanner, while having the merits of being (1) more affordable; (2) much easier to operate; and (3) more robust. Compared with the current practice of casting, our technology demonstrates its superiority because it (1) is non‐destructive; (2) collects more evidence detail than casts, especially when an impression is fragile; (3) requires less time and money to collect each piece of evidence; and (4) results in a digital file that can easily be shared with other examiners.     

“坚持以人民为中心”的创新理念和重大实践对民办高校思政教育队伍建设的启示

习近平总书记在福建工作期间形成了极为宝贵的创新理念和重大实践。这些理念和实践具有前瞻性、开创性、战略性,其中“坚持以人民为中心”的理念和实践对民办高校思想政治教育队伍的建设有重要的指导意义。目前,民办高校思想政治教育队伍存在理论学习不深入、学生思想动态调研不充足、专业技能学习不及时、融入学生群体不顺畅、思想政治辅导员队伍不稳定、辅导员与思想政治理论课教师之间的思想政治教育不融合等薄弱点。针对这些问题,应将“坚持以人民为中心”作为民办高校思想政治教育工作的理念和实践,将一切工作的初衷紧紧围绕着学生这个群体,促进思想政治教育队伍的建设。     

Entre la pampa del lenguaje y el alambrado de la escritura: la mancha roja como síntoma de la ambigüedad de lo real en La ocasión de Juan José Saer

La presencia recurrente del color rojo en La ocasión de Saer desempeña una función desestabilizadora en relación a la representación figurativa. En el artículo se establecen analogías entre esta función y el elemento voluble, no mimético de la pintura que el pensador e historiador de arte, Georges Didi-Huberman, denomina como “pan” en contraste con el detalle, categoría asociada con el carácter icónico de la pintura. Al igual que el “pan”, las manchas rojas en la novela apuntan a la dimensión de lo real que se resiste a la legibilidad y señala los límites de la narrativa realista. Saer se vale de contrastes cromáticos para aludir a tensiones políticas y epistemólogicas y a las paradojas de escribir una novela situada en el siglo diecinueve pero incorporando la experiencia literaria del siglo veinte.     

Postmortem chest computed tomography for the diagnosis of drowning:a feasibility study

It may be difficult to distinguish the cause of death in drowning cases without specific findings. The aim of this study was to explore the forensic value of thoracic postmortem computed tomography (PMCT) using routine images and three-dimensional (3D) image reconstructions. The imaging data of PMCT examinations of six drowning cadavers, aged 21–54 years, were analyzed. Twelve victims of sudden death from coronary artery disease (CAD) were chosen as a control group. After 3D bilateral lung images were reconstructed using image processing software, an interactive medical image control system was used to measure and analyze parameters including lung volume, lung volume ratio, mean CT value of the whole lung, and lung CT value distribution curves. Lung volume and lung volume ratio were used to assess the shape changes of the lung. Lung CT value distribution curves showed the corresponding number of pixels of the different CT values in the lung image. Lung volume was not significantly larger in drowning cases (mean 2 958 cm³) than in controls (mean 2 342 cm³). Lung volume ratio values in the drowning group (mean 0.3156) were greater than those in the control group (mean 0.2763); (P = 0.02). There was no significant difference between the drowning and control group in the mean CT value of the whole lung. There were differences between lung CT value distribution curves in drowning victims and controls, with drowning victims showing a single peak and CAD cases showing a bimodal distribution. Thoracic PMCT is helpful for the forensic medical diagnosis of drowning. Lung volume ratio and lung CT value distribution are potential indicators to distinguish between drowning and CAD.     

历史中的情势与偶然——马克思基于唯物史观的政治哲学探讨

《1848年至1850年的法兰西阶级斗争》和《路易·波拿巴的雾月十八日》不仅被阐释为政治学著作和历史学著作,而且是马克思把唯物史观的一般规律具体化到政治哲学领域中的典范之作,特别是其对当时法国政治事件中"活生生的时事"的内涵和外在表现深入细致地剖析,是实现了唯物史观的基本理论与社会现实之政治事件相统一的哲学著作.对这两...     

Virtual anthropology: a preliminary test of macroscopic observation versus 3D surface scans and computed tomography (CT) scans

Virtual anthropology (VA) is based on applying anthropological methods currently used to analyse bones to 3D models of human remains. While great advances have been made in this endeavour in the past decade, several interrogations concerning how reliable these models are and what their proper use should be remain unanswered. In this research, a fundamental assumption of VA has been investigated: if the way we perceive and apply an anthropological method is truly similar when looking at bones macroscopically and through various 3D media. In order to answer, 10 skulls of known age and sex were scanned using a computed tomography (CT) scanner and a 3D surface scanner. Two observers separately applied a defined staging method to eight suture sites on these skulls, first looking at the bone macroscopically, then at the 3D surface scan, and finally on the CT scan. Two rounds of observation were carried out by each observer. Intra- and inter-observer error were evaluated, and two sample t-tests used to evaluate if the different types of medium used yielded significantly different observations. The results show a high degree of inter-observer error, and that data obtained from 3D surface scans differ from macroscopic observation (confidence level 95%, P ≤ 0.05). CT scans, in these settings, yielded results comparable to those obtained through macroscopic observations. These results offer many possibilities for future research, including indications on the kind of anthropological methods and anatomical landmarks that might be reliably transferable to the virtual environment. All current methods used in traditional anthropology should be tested, and if they prove unreliable, new techniques to analyse bones from virtual models should be developed.

Key points

• Large discrepancies between observation on dry bones and computer-generated 3D models (surface scans or CT scans) could lead to the re-evaluation of the suitability of traditional anthropological methods for application on 3D models.
• This preliminary study evaluates whether macroscopic, 3D surface scans, and CT scans viewings generate different observations.
• The results indicate that the data are not always coherent across all three media of observation.
• Explanations include the aspect given to the bone by the 3D software, differences between handling bones in real life versus on a computer, and level of expertise of the observers.

     

Empirical Evaluation of the Reliability of Photogrammetry Software in the Recovery of Three-Dimensional Footwear Impressions

This paper examines the reliability of Structure from Motion (SfM) photogrammetry as a tool in the capture of forensic footwear marks. This is applicable to photogrammetry freeware DigTrace but is equally relevant to other SfM solutions. SfM simply requires a digital camera, a scale bar, and a selection of oblique photographs of the trace in question taken at the scene. The output is a digital three-dimensional point cloud of the surface and any plastic trace thereon. The first section of this paper examines the reliability of photogrammetry to capture the same data when repeatedly used on one impression, while the second part assesses the impact of varying cameras. Using cloud to cloud comparisons that measure the distance between two-point clouds, we assess the variability between models. The results highlight how little variability is evident and therefore speak to the accuracy and consistency of such techniques in the capture of three-dimensional traces. Using this method, 3D footwear impressions can, in many substrates, be collected with a repeatability of 97% with any variation between models less than ~0.5 mm.     

Development of a Novel Finger-Trigger Interface for Trigger Pull Measurement

Trigger pull is the force that needs to be exerted on the trigger to discharge a firearm. The measurement of trigger pull can assist in the evaluation of the safety, function, and manufacturing characteristics associated with a firearm during the forensic firearm examination process. Nonetheless, the accuracy and uncertainty of trigger pull measurements may be affected by the measuring device, test procedure, and environmental conditions. In this work, an innovative finger-trigger interface device was developed to facilitate accurate trigger pull measurements. The idea was to reduce the variation related to the position of the measurement device on the trigger in existing measuring methods and devices. Three force sensors based on different technologies were initially evaluated. While two of the three sensors failed to produce data, the miniature capacitive plate sensor exhibited high precision and a linear response over the range of typical trigger pulls. To examine the effects of the finger-trigger interface on trigger pull measurement, different sensor housing prototypes were designed in silico and 3D printed for the construction of three finger-trigger interface devices. The performance of each finger-trigger interface device was evaluated by measuring the trigger pulls of several selected firearms and comparing the data to a previously published study. Our preliminary results demonstrated the novel finger-trigger interface device offered a new way to measure trigger pull in situ with acceptable accuracy and precision.     

农村承包地三权分置制度入典研究

《农村土地承包法修正案》将“三权分置”政策内容上升为法律规定,确立承包地三权分置制度。对解决承包地流转闭锁抵押难行等问题意义重大。却因将承包经营权本集体经济组织内封闭流转以法律固化,新设土地经营权性质不清、类型混合、流转不济,无益承包地债权性与物权性并可市场化开放性流转之“三权分置”目标实现。受其影响,《民法典分编(草案)》亦存同样问题。亟待农村承包地三权分置制度进一步入典完善。在实地调研基础上,遵循《民法总则》落实集体土地所有权前提下,于《民法典分编(草案)》完善中,基于英美地产权客体权益分离理论与大陆法系二次权能分离理论具兼创债权性与物权性经营权功能的地权二次分离理论,在保持土地承包经营权规定不变上,从其客体权益中分离出二元化土地经营权,并分别于合同编增设农地租赁经营合同具体规范债权性经营权、于物权编构造“农用地使用权”科学规范物权性经营权。以利能以债权性经营权顺农地灵活经营实践、物权性经营权应农地抵押及长期经营所需的二元化路径,促现行承包经营权本集体经济组织内封闭流转向派生出的土地经营权市场化开放性流转发展,以实现农村承包地三权分置制度入典达成“三权分置”、“放活土地经营权”之政策目标。