口蹄疫病毒P1 2A和3C基因重组逆转录病毒表达载体的构建

以A型口蹄疫病毒(FMDV)AV88(L)和XJ99株的P12X基因和AV88(L)3C基因的阳性克隆质粒为模板,用PCR扩增各基因片段,酶切后分步定向亚克隆至逆转录病毒表达载体pBABE-puro上经PCR、酶切鉴定及测序。结果表明,获得了2个含不同RGD基序的A型FMDV衣壳蛋白和蛋白酶基因的重组逆转录病毒表达载体pBABE-AV88(L)P1 2X3C和pBABE-XJ99 P1 2X3C,为研究安全、高效、低成本的FMDV空衣壳亚单位疫苗奠定了基础。     

美国打击恐怖主义融资的刑事立法与调查介评

9.11事件后,对恐怖主义融资加大刑事打击使美国成为国际刑事司法界开展金融反恐的参照样本。美国的反恐融资执法机构重组、刑事立法及时跟进、加大刑事措施力度和权限,以及具体的办案经验,以期为我国的打击恐怖主义融资刑事对策提供经验和借鉴。     

身份叙事与中国参与北极事务身份建构

国家身份可以划分为总体身份和具体身份,具体身份是总体身份在不同场域的具体投射。作为话语实践的一种形式,叙事则是身份建构的主要机制,它将一系列关于总体身份和具体身份的隐喻以特定逻辑联系在一起。当代中国所追求的总体身份是负责任大国,而这一身份的主要表现则是倡导构建人类命运共同体。这一身份通过领域或功能以及地域两个维度被投射到北极场域,形成了"北极事务的重要利益攸关方"这一具体身份。本文在论述叙事和国家身份形成关系的基础上,重点分析了"北极事务的重要利益攸关方"这一身份隐喻通过叙事被投射到具体场域中的过程。通过叙事,"北极事务的重要利益攸关方"同中国的国际责任、各国的共同利益等话语联系在一起,成为负责任大国身份在北极场域的延伸,成为构建人类命运共同体这一宏观目标的组成部分。     

论中国应该实行准结盟战略

准结盟战略是一种防御性外交战略,是在某种特殊情况下,在某些重大问题上与意见相同的国家达成共识而采取的一种临时性、阶段性的紧密合作措施.世纪之交,北约东扩、日美安保条约西扩、中美关系很不确定、国际力量对比严重失衡等为建立准结盟战略提供了条件.目前,中国应把发展与俄、印度、印尼等国家的关系放在建立准结盟战略的首位.     

The Establishment of the Philosophy of Comedy in The Cherry Orchard

A. P. Chekhov, known as “the pioneer of modern drama in the 20th century”, is not only a famous Russian anovelist but also an outstanding dramatist. The Cherry Orchard is the author’s last play. In this paper, we made a detailed analysis of the text. First of all, the paper analyzes its “joy in grief” comedy philosophy from the so-called “sadness”. Secondly, the paper discusses the transformation from a real existence to the metaphorical process of “the cherry orchard” in combination with the author’s life. Finally, this paper analyzes the surface structure and deep structure of the play from two aspects of portraits of characters and scenes and reveals the way to establish the philosophy of comedy in this play.     

系列性案件侦查理论三：有限空间理论

有限空间理论是根据系列性案件的犯罪主体跨区域、大范围、跳跃式作案的特点,以案件自身的发展规律为依据,预测犯罪发生和可能发生的地点,进行预警和围追堵截,最终将犯罪人抓获归案的理论。有限空间理论是建立在信息源、同心圆理论的基础之上的,在系列性案件侦查中占有极为重要的地位。     

The delisting decision: The case of buyout offer with squeeze-out (BOSO)

In this paper, we exploit the specificity of going-private transactions that are initiated by the historic controlling shareholders (i.e. voluntary delistings). In Continental Europe, the majority of firms that become private do so following a buyout offer with squeeze-out (BOSO); using this mechanism, the controlling shareholder can cash out minorities and take the firm private. We argue that the decision to go private results from a cost–benefit analysis. Moreover, we pay particular attention to the consequences and the related costs of compliance resulting from the passage of the French Financial Security Law (FSL) in 2003. A quantitative study was performed using a unique dataset spanning 1997–2006. This data set consists of 140 French firms, of which 70 were voluntarily delisted via BOSO and 70 were industry-matched control firms. Univariate analysis and logistic regressions support the cost–benefit analysis: when listing benefits decrease because of weak liquidity and/or weak analyst coverage, it seems better for the firm to go private. Furthermore, the inherent characteristics of delisted firms (i.e. performance, leverage, and risk as measured by the beta factor) appear to be important driving factors of delisting. The passage of the FSL has strengthened the impact of these characteristics on the decision to go private. Mature firms that have weak performance and low specific risk and that are not financially constrained by debt will decide to go private because they cannot afford the listing status anymore. Finally, we show that the driving factors of delisting differ according to the identity of the controlling shareholder; specifically, the level of risk appears to be the strongest determinant for family firms, while non-family firms also consider their own financial structure.     

基于公平的包容性增长:科学内涵、约束条件及路径选择

经济增长虽然对减少贫困是必须的,但并非是充分的。基于动态的结构性经济变革的可持续和平等增长,对于实质性的削减贫困是必需的;它能更快地促进联合国千年发展目标的实现。作为动态的结构性的一种经济增长,包容性增长在我国应当受到重视。包容性增长与科学发展、和谐发展一脉相承。本文阐述了包容性增长的演进及科学内涵,结合中国实际情况对其实现的路径选择提出了建议。     

单克隆抗M、抗N抗体的研制及初步应用

用人血型糖蛋白 A 和人红细胞分别免疫 BALB/C 小鼠。用被免疫的 BALB/C 小鼠脾细胞与 SP2/0骨髓瘤细胞融合,在37℃、5%二氧化碳培养箱中孵育10～15天,然后用 OM 和 ON 型指示红细胞分别检测培养上清液的凝集情况,筛选出能分泌高特异性和高效价抗 M 和抗 N 抗体的细胞株,并建立了 GM_4H3、GM_4H_4、N_2A_3和 N_2D_(10)细胞株。这些细胞株可持续分泌免疫球蛋白 G 类抗 M、抗 N 抗体。应用这些抗体,通过血凝法、解离法和 ELISA 斑点法,可进行血及血痕的 MN 分型。在血型检验中,优于多克隆抗 M、抗N 血清。     

口蹄疫病毒非结构蛋白3A单克隆抗体的制备和鉴定

以原核表达并纯化的口蹄疫病毒(FMDV)非结构蛋白3A免疫BALB/c小鼠,取其脾细胞与小鼠骨髓瘤细胞SP2/0进行融合,用间接ELISA方法筛选杂交瘤细胞,有限稀释法进行亚克隆,获得3株能稳定分泌抗3A蛋白单抗的杂交瘤细胞,分别命名为3H2、4B1、7F5,并对这3株单抗进行了生物学鉴定。结果显示,单抗3H2和4B1为IgG2a亚型,7F5为IgG2b亚型。单抗7F5针对的表位与3H2和4B1两株单抗的表位不一致;这3株单抗均能够与FMDV特异性结合,且单抗4B1与所有3A相关蛋白均反应。稳定性鉴定结果表明,获得的3株杂交瘤细胞均能稳定分泌单克隆抗体。研究结果为进一步探索3A蛋白的结构和功能以及建立诊断方法奠定了基础。