鸡贫血病毒VP1、VP2和VP3基因酵母双杂交载体的构建及其自激活活性的鉴定

为构建鸡贫血病毒(CAV)VP1、VP2、VP3基因酵母双杂交载体并鉴定其自激活作用,从CAV细胞传代毒M9905毒株中提取基因组DNA,利用PCR分别扩增VP1、VP2和VP3基因,采用Gateway技术将VP1、VP2和VP3基因分别克隆到酵母双杂交载体pDEST32和pDEST22中,构建了诱饵载体及猎物载体,经酶切和PCR鉴定且测序正确后,用醋酸锂法转化酵母菌株Mav203,通过缺陷型平板SD/-Leu/-Trp/-His和SD/-Leu/-Trp/-Ura筛选以及LacZ报告基因检测载体有无自激活作用。结果表明,成功构建了诱饵载体pDEST32-VP1/VP2/VP3和捕获载体pDEST22-VP1/VP2/VP3,并证明其VP1、VP2和VP3蛋白在酵母双杂交系统中无自激活作用。研究结果为下一步利用酵母双杂交系统探索CAVVP1、VP2、VP3蛋白之间的相互作用奠定了基础。     

大学文化视角下的大学生思想政治教育

大学文化在高校大学生思想政治教育中扮演重要角色,对大学生具有理想信念确立、民族精神培育、公民道德养成等独特影响,赋予了大学生思想政治教育文化熏陶、思想拓展、感情疏导等新的功能。推动大学文化和大学生思想政治教育互动发展成为高校培养优秀人才的新趋向。     

互动:操纵的传媒与传媒的操纵——浅析新闻传媒与美国政治之间的相互影响

在美国,新闻作为一种特殊的政治力量,在一定程度上改变着政治结构,而政治又在某种程度上影响着新闻的形成.这使得新闻传媒自觉或不自觉地成为一种被操纵的工具;而传媒也反过来影响政治及统治者本身.政治与传媒的互动形成“你中有我、我中有你”的动态平衡,从而使传媒政治化、政治社会化.     

论宪法与行政法的统一与互动——兼谈宪法与行政法在公法体系中的地位

统一公法学的提出,展现了公法所及问题的广泛性、复杂性和系统性,需要从整体公法视角研究各部门公法之间的关系,尤其要深入研究宪法与行政法的关系.宪法与行政法是公法体系的基本组成部门,宪法居于核心和统领的地位,行政法居于主角和关键的地位.宪法与行政法的关系最密切,集中表现为二者最大的一致性和动态推进的互动性,正是这种统一互动的关系引领和推动着整个公法体系的建构与完善.     

论私力救济与公力救济的交错——一个法理的阐释

本文挑战了私力救济与公力救济关系的流行观念 ,提出两者之间关系密切、交错互动 ,并从其相互影响和相互转化等方面作了详尽阐述 ,为公力救济、司法、以及法治等问题提供了一个全新视角     

The effects of procedural aspects and outcome salience on procedural fairness judgments

The effects of process control and decision control on procedural fairness judgements are examined with regard to the procedure used by commercial banks in granting business credits to entrepreneurs. Male and female entrepreneurs with experience in requests for business credits were interviewed about several aspects of the procedure for granting business credit. Respondents had either a positive or a negative experience with credit granting (i.e., the request was or was not rewarded). The outcome-oriented and the procedure-oriented explanations for the effects of process control on procedural fairness judgments are discussed. The results show that, contrary to expectation, process control had no effect on the procedural fairness judgments. On the contrary, perceived seriousness of treatment, as well as the predicted effects of decision control, did influence procedural justice judgments. Moreover, some support was found for the contention that seriousness of treatment functions as precondition for process control effects (Tyler, 1987). Neither the outcome-oriented, nor the procedure-oriented explanation could fully account for the findings. It is assumed that the specific aspects of the situation are responsible for the results, indicating how important the situational context is in research concerning procedural justice.     

Charge reduction: An analysis of prosecutorial discretion in burglary and robbery cases

Although a substantial number of researchers have studied charge reductions taking place within the context of guilty plea negotiations, few have focused on estimating the determinants of charge reductions taking place at the initial screening decision. The prosecutor's decision to reduce original felony charges to a misdemeanor has serious social, legal, and economic consequences for the suspect. This paper presents a model of the variables affecting the likelihood of such a reduction in burglary and robbery offenses. Drawing from Littrell's principled charging perspective and earlier research on labeling, the analysis involves estimating logistic regression equations specifying both main and interaction effects of the suspect's gender and race and variables related to suspect character, case seriousness, and legal seriousness. Partial support is found for Littrell's perspective.     

彝民通过电视的仪式--对一个彝族村落"电视与生活"关系的民族志研究

通过把电视植入某个社区的过程视为该社区通过电视的仪式,可以将大众传播视为社区在现代宿命的、必然要"通过"的"仪式",是在面临大众传媒、尤其是电视所带来的种种变化和现象时,社区为标识、定位、解释这些现象和变化,进而调整自己以适应这种现象和变化而做出的种种社会性行为.展示一个彝族村落中"电视与生活"关系的民族志表明电视与村落的生活一直处于相互建构的过程中,村民按照自己的方式将电视纳入当地生活,同时村民们也渐渐调整自己的生活和文化结构以适应电视.     

清季民国戏剧改良与妇女解放的互动关系考察——以河南为例

清季民初，戏剧改良运动兴起，其宗旨为移风易俗，妇女解放便是其中一项内容。清末，作为戏剧大省的河南开始允许妇女入园看戏，民国时期，女演员登台演戏日益增多，她们成名后积极义演赈灾、支持教育，一定程度上提高了妇女的社会地位。通过对河南戏剧改良的梳理与考察可知：戏剧改良一定程度上推动了妇女解放，妇女解放也促进了戏剧的革新，戏剧改良与妇女解放互为促进。     

刑法理论与实践之间的“恶性循环”与“良性互动”——我国刑法理论的反思

根据我国刑法理论与实践之间的关系,可以把我国的刑法理论分为与实践"恶性循环"的理论和与实践"良性互动"的理论。前者以犯罪构成体系的争论来说明,后者以死刑的改革来说明。我国刑法理论在国外刑法学理论的"刺激一反应"模式之下缺乏理论的主体性和独立性,不但没有自己的问题意识,而且不能正确对待国外的刑法学理论。应该反思"恶性循环"的刑法学理论,应该提倡"良性互动"的刑法学理论。