死亡人体胸肌组织中rRNA亚基的稳定性评价

目的观察尸体胸大肌组织中rRNA各亚基表达水平的死后稳定性,探讨其在死亡时间推断中应用的价值。方法选取6例（3例成人、3例婴幼儿）外界温、湿度环境基本一致、死亡时间基本一致（24h以内）的个体,提取左侧胸大肌组织,在PBS缓冲液中低温冻存,分别在尸检即刻、2、3、4、5、6、7、8、9、10d提取微量组织保存于RNA固定液中,通过实时荧光定量RT-PCR技术检测rRNA 5srRNA、5.8srRNA、18srRNA、28srRNA亚基的表达水平。并分析年龄、死因对rRNA亚基表达水平的影响。结果死后各时间点rRNA各亚基的表达随死后经过时间延长无显著性降解,Ct值变化与死后经过时间无相关性（P值均大于0.05）,其中5s rRNA在18.503±2.655~20.937±2.340之间,5.8s rRNA在17.687±3.011~20.617±2.204之间,18s rRNA在16.457±3.920~22.330±2.571之间,28s rRNA在15.077±6.051~22.207±2.685之间。结论人体胸肌组织中rRNA各亚基稳定性好,其各亚基的表达量与死因、年龄无关,适于作为晚期死亡的推断的内参基因。     

How reliable are eyewitness memories? Effects of retention interval,violence of act,and gender stereotypes on observers' judgments of their own memory regarding witnessed act and perpetrator

An experiment was conducted to evaluate the effects of (i) stimulus person's gender, (ii) type of act (neutral or violent), and (iii) retention interval (short or long) on observers' memory of a stimulus person. Participants were presented with one of two acts: neutral (walking around in a store) or violent (robbing a store). The retention interval was 10 minutes or one–three weeks. The dependent variables were questionnaire items concerning the participants' memory of (1) the stimulus person's appearance and (2) the event, and (3) rating scales where the participants were asked to evaluate the stimulus person's aggressiveness, insensitivity, and other personality traits as well as characteristics of the act. Results showed that when the act was violent, and a long retention interval was used, a female, but not a male, stimulus person was evaluated less harshly than with a short retention interval (enhancement of gender stereotype); a stimulus person was seen as behaving in a more masculine way when performing a violent rather than a neutral act; witnessing the violent act resulted in better self-rated memory of the stimulus person; and with increasing retention interval, the violent act was seen as less negative and the neutral act as more negative (regression toward the mean).     

The Time Path of Legislative Party Switching and the Dynamics of Political Competition: The Italian Case (1996–2011)

Political competition is more realistically described as a dynamic process rather than as a series of static stages in which parties compete over policy and government formation. This paper focuses on legislative party switching as the main manifestation of this endogenously evolving process, linking individual switching behaviour to policy and office incentives that are assumed to evolve throughout the life of the entire legislature. Using a new data set tracking the timing of MPs’ changes in party affiliations between 1996 and 2011 in Italy, it is found that switching is mainly motivated by policy reasons and that it is more likely during government formation periods and budget negotiations. These results are a consequence of the interplay between MPs’ ambition and the alternation of key phases in the legislative cycle.     

Comparing trends in infanticides in 28 countries, 1960–2009

The term ‘infanticide’ refers to the homicide of a child younger than one year old. In this article, we describe infanticide trends in 28 industrialized countries between 1960 and 2009. The analysis is based on the cause of death data from the WHO Mortality Database and national materials. The purpose is to compare those trends in all these 28 countries not to compare countries per se. Cause of death statistics are based on ICD classification. During the review period, ICD classification changed three times. In addition to describing infanticide trends, we will analyse the impact of those changes on statistical infanticide levels, to be sure that changes in trends could be seen as real, not statistical artefacts. According to our analysis, the change from ICD-7 to ICD-9 in 1968–1970 seems to have had some impact on registered infanticide mortality levels in three of the studied countries. In other countries, the changes did not have any general impact on registered infanticide levels. During the period, infanticide rates decreased in almost all European countries, and increased or were stable in most non-European industrialized countries. Even in Europe, there were significant differences in the decreasing trend between countries. We also found some structural variation behind the trend figures, which raised the question of whether the aggregated infanticide levels really describe an identical phenomenon in different countries.     

Dépendance et luttes politiques au Kenya, 1975–1977: Ia bourgeoisie nationale à l'assaut du pouvoir d'État

Rural West Africans confront a challenging array of circumstances in pursuit of their livelihoods, which are negotiated through repertoires of knowledge, skill, wisdom and information that are held individually, communally or as the prerogative of experts. Of some significance is timing: finding the most effective or propitious moment for action. On the farm, anticipating the beginning and progress of the rains is crucial and sought through the empirical observation of natural phenomena, or as in northwest Nigeria additionally through agricultural star calendars and almanacs by Muslim clerics. Furthermore, some clerics use divination to identify propitious moments for their clients whereby they may achieve success in non-farm jobs and businesses that are an integral part of rural livelihoods. Taking action to achieve particular outcomes on and off the farm raises issues about local people's understanding of the physical world and their place within it, and the power of religion and the supernatural. An appreciation of such matters would appear to be crucial for those from the scientific and technological North who may be attempting to transform African societies.     

PUNITIVE SENTIMENT

Scholarship has long noted the importance of understanding the changes that occur over time in aggregate public support for punitive criminal justice policies. Yet, the lack of a reliable and valid measure of this concept limits our understanding of this aspect of the criminal justice system. This research develops a measure of public support for punitive policies from 1951 to 2006 using 242 administrations of 24 unique survey indicators. It argues that punitive sentiment is politically constructed via frames focusing on the permissiveness of the criminal justice system. Punitive sentiment is estimated with an error‐correction model showing both the short‐ and long‐term relationships between punitive sentiment and presidential framing of crime, public dissatisfaction with social welfare policies, and perceptions of racial integration. The results highlight the complex dynamics responsible for the change over time in punitive sentiment as well as the possibilities of obtaining public support for alternative solutions to crime.     

Optimizing Accu Time‐of‐Flight/Direct Analysis in Real Time for Explosive Residue Analysis

The use of a direct analysis in real time (DART) mass spectrometer (MS) instrument was optimized for 22 compounds of organic explosive residues to provide a guide for DART‐MS users in rapid screening of explosive compounds. Samples were introduced as neat solutions and sequential dilutions to determine optimal instrument conditions and lowest concentration detectable. Most compounds were optimized to 250°C in the negative ion mode, and several compounds benefited from the addition of a chloride dopant from methylene chloride (amino‐dinitrotoluenes, RDX, EGDN, and PETN). Few compounds were more sensitive in the positive ion mode (TEGDN, DEGDN, HNS, and DMNB). Mixtures of compounds were detected using clean room wipes, directly from their surfaces and from subsequent extractions. Compounds from the mixtures were also successfully detected in soil and from swipes of spiked surfaces. The instrument showed merit in detection of pg/μL solutions for most of the compounds and among the substrates tested.     

The end of the millennium or the countdown

In the millennial countdown the time remaining is already past, and the maximal utopia of life gives way to the minimal utopia of survival. This is the paradox of the achieved utopia which puts an end to the utopian dimension. It creates an impossible situation, in the sense that it exhausts historical possibilities.     

网络环境中公民参与的时代价值及路径选择

以互联网为核心的信息网络技术革命更加凸显了公民参与的时代价值,同时也给公民参与带来了许多困境。全面分析网络环境中公民参与的尴尬局面,寻求摆脱这些困境的路径选择,对于引导中国公民理性地进行参与活动,促进中国公民参与的有序化发展具有重要的理论和现实意义。     

CDK5的表达与脑损伤时间推断的相关性研究进展

细胞周期蛋白依赖激酶5(cyclin-dependent kinase 5,CDK5)是CDK家族中一类不直接调控细胞周期的蛋白。CDK5通过靶蛋白的磷酸化在神经细胞的发育、修复及退行性变等方面发挥着不可替代的作用。脑损伤是指暴力作用于头部造成的脑组织器质性损伤,由于损伤发生后机体启动应激及修复机制,脑组织中变化的多种蛋白及酶类物质可作为损伤时间判定的指标。本文就CDK5的分布、活性机制、生理作用及其脑损伤研究与其作为脑损伤标志物可能性的研究进展进行综述。