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871.
为了研究猪Toll样受体3(pTLR3)基因的选择性剪切机制,应用反转录-聚合酶链反应扩增技术,从猪外周血淋巴细胞中克隆了pTLR3及其剪接体基因(pTLR3a,pTLR3b)。pTLR3基因序列分析表明,克隆的基因ORF为2 718bp,编码905个氨基酸;推导的氨基酸序列分析显示,在其胞外区具有LRRs结构域,胞内区含有TIR结构域,具有TLR家族的典型结构特征。剪接体分析显示,pTLR3a和pTLR3b基因缺失第3外显子,都没有可编码跨膜区、胞内区的部分。相似性分析显示,与牛、马、猫和人的相似性较高,与家兔、鼠的相似性次之。证实pTLR3可变剪接体的存在为进一步研究其功能奠定了基础。 相似文献
872.
Min Yang Ph.D. Li Mou M.S. Yi‐Ming Fu M.S. Yu Wang Ph.D. Jiang‐feng Wang Ph.D. 《Journal of forensic sciences》2019,64(5):1324-1334
This study was designed to establish a feature identification method of tool‐mark 2D data. A uniform local binary pattern histogram operator was developed to extract the tool‐mark features, and the random forest algorithm was adopted to identify these. The presented method was used to conduct five groups of experiments with a 2D dataset of known matched and nonmatched tool‐marks made by bolt clippers, cutting pliers, and screwdrivers. The experimental results show that the proposed method achieved a high rate of identification of the tool‐mark samples generated under identical conditions. The proposed method effectively overcomes the disadvantage of unstable illumination of 2D tool‐mark image data and avoids the difficulty in mark inspection caused by manually preset parameters in the existing methods, thus reducing the uncertainty of inspected results. 相似文献
873.
Isabella Aquila M.D. Ph.D. Matteo A. Sacco M.D. Giuseppe Aquila M.S. Roberto Raffaele M.S. Alfredo Manca Giuseppe Capoccia Fabrizio Cordasco M.D. Pietrantonio Ricci M.D. Ph.D. 《Journal of forensic sciences》2019,64(5):1540-1543
Photographic surveying is fundamental to crime scene investigation. It is performed using 2D images; however, this method is limited because it does not offer a detailed view. We present a 3D (three‐dimensional) experimental model that has clarified unsolved aspects of a homicide. A woman was found dead in her home. Considering the large number of injuries on the victim, signs of the use of two weapons, and the absence of traces of the killer/s, several hypotheses about the dynamics and the number of offenders were made. The forensic surveys suggested that a single offender had used two weapons at the same time, but this hypothesis was considered to be impossible by investigators. Our 3D model allowed us to experimentally reproduce the murder and compare various dynamics. The model was used as evidence in Court to prove the hypothesis of a single killer and demonstrate that no other people were involved. 相似文献
874.
Pierre Duez M.A.Sc. Todd Weller M.S. Marcus Brubaker Ph.D. Richard E. Hockensmith B.S. II Ryan Lilien M.D. Ph.D. 《Journal of forensic sciences》2018,63(4):1069-1084
The transition from 2D imaging to 3D scanning in the discipline of firearms and toolmark analysis is likely to provide examiners an unprecedented view of microscopic surface topography. The digital examination of measured 3D surface topographies has been referred to as virtual microscopy (VM). The approach offers several potential advantages over traditional comparison microscopy. Like any new analytic method, VM must be validated prior to its use in a crime laboratory. This paper describes one of the first validation studies of virtual microscopy. Fifty‐six participants at fifteen laboratories used virtual microscopic tools to complete two proficiency‐style tests for cartridge case identification. All participating trained examiners correctly reported 100% of the identifications (known matches) while reporting no false positives. The VM tools also allowed examiners to annotate compared surfaces. These annotations provide insight into the types of marked utilized in comparative analysis. Overall, the results of the study demonstrate that trained examiners can successfully use virtual microscopy to conduct firearms toolmark examination and support the use of the technology in the crime laboratory. 相似文献
875.
At outdoor crime scenes, cadaver-detection and blood-detection dogs may be tasked with locating blood that is days, weeks or months old. Although it is known that the odour profile of blood will change during this time, it is currently unknown how the profile changes when exposed to the environment. Such variables must be studied in order to understand when the odour profile is no longer detectable by the scent-detection dogs and other crime scene tools should be implemented. In this study, blood was deposited onto concrete and varnished wood surfaces and weathered in an outdoor environment over a three-month period. Headspace samples were collected using solid phase microextraction (SPME) and analysed using comprehensive two-dimensional gas chromatography – time-of-flight mass spectrometry (GC×GC–TOFMS). The chemical odour profiles were compared with the behavioural responses of cadaver-detection and blood-detection dogs during training. Data interpretation using principal component analysis (PCA) and hierarchical cluster analysis (HCA) established that the blood odour could no longer be detected using SPME–GC×GC–TOFMS after two months of weathering on both surfaces. Conversely, the blood-detection dogs had difficulty locating the blood samples after one month of weathering on concrete and after one week of weathering on varnished wood. The scent-detection dogs evaluated herein had not been previously exposed to environmentally weathered blood samples during training. Given that this study was conducted to test the dogs' baseline abilities, it is expected that with repeated exposure, the dogs' capabilities would likely improve. The knowledge gained from this study can assist in providing law enforcement with more accurate training aids for blood-detection dogs and can improve their efficiency when deployed to outdoor crime scenes. 相似文献
876.
Sailer 《Juristische Bl?tter》2008,130(3):184-185
Die "gesetzlichen Zinsen", deren Rückzahlung § 27 Abs 3 MRG anordnet, unterliegen – unbeschadet der für die Rückforderung
geltenden Verj?hrungsbestimmungen von drei bzw zehn Jahren – jedenfalls der Verj?hrungsfrist des § 1480 ABGB. 相似文献
877.
Mizuno N Kitayama T Fujii K Nakahara H Yoshida K Sekiguchi K Yonezawa N Nakano M Kasai K 《Journal of forensic sciences》2008,53(5):1068-1073
Short tandem repeat studies are powerful tools for parentage analysis and for identification of missing persons, victims of murder, and victims of mass fatalities when reference samples are unavailable. The primer in the Identifiler kit failed to amplify an allele at the D19S433 locus, producing a silent ("null") allele. The causal mutation is a base change (G>A) 32 nucleotides downstream from the 3' end of the AAGG repeats. The silent alleles are problematical in parentage analysis because when transmitted, they can cause a parent-child inconsistency that is unrelated to Mendelian genetics. The inconsistency is sometimes termed an "apparent opposite homozygosity" and it produces false evidence of nonparentage. Alternative primers were designed to amplify the D19S433 locus alleles and they detect the silent allele. Frequencies of the (no longer) silent allele were determined to be 0.0114 in 176 people from Shizuoka (Honshu) and 0.0128 in 156 people from Okinawa. 相似文献
878.
罗祥远 《长沙民政职业技术学院学报》2003,10(2):84-86
本文探讨了SMTP协议和POP3协议的工作原理和工作过程以及通过SMTP协议利用VB的WinSock从控件发送电子邮件的客户端程序的编写步骤。 相似文献
879.
Adele V. Harrell 《Journal of Experimental Criminology》2006,2(3):339-344
This response argues that the National Research Council (NRC) report, while valuable and thorough, would have benefited from
conceptualizing evaluation activities along a continuum of knowledge development, with evaluations initially verifying the
effectiveness of program concepts, before moving to evaluations of the feasibility and generalizability of anti-crime strategies
and, finally, to evaluations of the costs and benefits of implementation of new strategies.
相似文献
Adele V. HarrellEmail: |
880.
科学马克思主义观的深化与拓展--论党的第三代中央领导集体的马克思主义观 总被引:1,自引:0,他引:1
党的第三代中央领导集体的马克思主义观,经历了一个确立、成熟和深入发展的阶段.从发生论、本质论、结构论、特征论和价值论等方面回答了什么是马克思主义的问题;从认真地学习和研究、完整准确地理解、既坚持又发展、不断进行理论创新、理论联系实际等方面说明了怎样对待马克思主义的问题.这一科学的马克思主义观具有重要的理论价值和实践意义. 相似文献