皮肤切创愈合过程中TLR2、TLR4表达及其法医学意义

目的探讨皮肤切创组织中单核-巨噬细胞TLR2和TLR4阳性表达与损伤时间的相关性,以寻找损伤时间推断的新方法。方法应用免疫组织化学技术研究45例不同损伤时间小鼠皮肤切创组织中TLR2、TLR4的表达情况,同时以3例正常小鼠皮肤组织作对照。结果皮肤切创组织中单核-巨噬细胞TLR2和TLR4阳性表达与损伤时间具有相关性。单核-巨噬细胞TLR2、TLR4阳性细胞比率(单核-巨噬细胞阳性细胞数与中性粒细胞、单核-巨噬细胞及成纤维细胞三种阳性细胞总数之比率)与损伤时间的回归方程分别为y=23.8099 0.9572x-0.0077x2 1.5×10-5x3(R2=0.872);y=25.2256 0.7893x-0.0059x2 1.0×10-5x3(R2=0.879)。结论TLR2、TLR4在小鼠皮肤切创愈合过程中损伤区内单核-巨噬细胞阳性表达比率与损伤时间相关;检测损伤区单核-巨噬细胞TLR2、TLR4的表达可望成为推断损伤时间的新方法。     

Abwehr von Eingriffen in das Mietrecht – zulässige Verfahrensart

Bei der Abgrenzung des streitigen vom au?erstreitigen Rechtsweg k?nnen nur konkrete bindende Absprachen über die in den §§ 8 und 9 MRG angeführten Rechte und Pflichten die Zul?ssigkeit des Rechtsweges ausl?sen. Enth?lt der Mietvertrag keine über das Gesetz hinausgehenden Regelungen, ist der Anspruch im au?erstreitigen Verfahren durchzusetzen. Zwar ist eine Behandlung der Klage als Antrag im Verfahren au?er Streitsachen nicht m?glich, wenn eine Gemeindeschlichtungsstelle besteht und daher vor der Anrufung der Gemeinde das au?erstreitige Verfahren vor Gericht unzul?ssig ist. Wies aber die Gemeindeschlichtungsstelle den entsprechenden Antrag ab und erhob die Partei binnen 4 Wochen Klage, ist die Umdeutung zul?ssig.     

Genetics and genomics of core short tandem repeat loci used in human identity testing

Over the past decade, the human identity testing community has settled on a set of core short tandem repeat (STR) loci that are widely used for DNA typing applications. A variety of commercial kits enable robust amplification of these core STR loci. A brief history is presented regarding the selection of core autosomal and Y-chromosomal STR markers. The physical location of each STR locus in the human genome is delineated and allele ranges and variants observed in human populations are summarized as are mutation rates observed from parentage testing. Internet resources for additional information on core STR loci are reviewed. Additional topics are also discussed, including potential linkage of STR loci to genetic disease-causing genes, probabilistic predictions of sample ethnicity, and desirable characteristics for additional STR loci that may be added in the future to the current core loci. These core STR loci, which form the basis for DNA databases worldwide, will continue to play an important role in forensic science for many years to come.     

高效液相色谱法测定人血清中安眠酮及其苯甲基羟化代谢物的浓度

本文建立了用反相高效液相色谱法(RP-HPLC)测定人血清中安眠酮及其苯甲基羟化代谢物-2-甲基-3[2-(羟甲基)-苯基]-4(3H)喹唑酮(Ⅰ)的浓度。安眠酮浓度在1-35μg/ml范围内呈直线相关(相关系数r=0.9980;回归方程y=0.06324x—0.1029),方法回收率平均为102.08±9.987(SD)％(n=5),检出限为1ng。其代谢物Ⅰ按原型安眠酮的线性浓度测定其相对量。本法为测定中毒者体内安眠酮及其代谢物Ⅰ的血浓度提供可行的手段。     

Detection of saliva traces using test strips

The test strip Rapignost-Amylase (Behring) for the rapid determination of alpha-amylase in the urine is also suitable for the determination of salivary amylase in stains stored up to 6 weeks at room temperature. The stains are extracted with physiological saline (extraction time 30 min), then the application zone of the strip is wetted with the extract. Positive amylase-reaction is recognised as a reddish-violet colouration of the reaction zone. Biological stains with low amylase concentrations (urine semen, vaginal secretion, mucus) react amylase negative. The method is uncomplicated and can be completed within 30 min. The test strips are easily available and stable during storage. Therefore the determination of saliva with test strips should be preferred to the clinical methods if the storage times of the stain are not longer than 4-6 weeks. It is a suitable procedure to determine salivary stains for use in forensic biology.     

浅析高校教育教学资源网络与移动3G网络的整合

高校教育教学资源网络是基于校园网内部的一个功能复杂的网络,往往需要提供两大服务功能:提供校内共事服务和提供Internet接入服务.而3G服务能够同时无线传送声音(通话)及数据信息(电子邮件、即时通信等).一般地讲,将无线通信与国际互联网等多媒体通信结合的新一代移动通信系统,未来的3G必将与教育教学资源信息网络进行结合,WAP与web的结合是一种趋势.     

十八届四中全会法治思想中的民生理念

党的十八届四中全会对法的论述诠释了法的目标,即把中国建设成社会主义法治国家,解释了法律是治国重要的武器,好的法律体系和法律制度才能真正贯彻公平、正义,维护人民的各种权益。全会一再强调恪守立法为民思想,加强法律体系建设,以高标准、严要求,加强法律制度和法律执行队伍的建设。无论全会中论述法的目的,还是法的权威及法律实施的各项要求,无一不透漏着党的民生思想,展现着以民为先、为民服务的思想。民生思想贯穿十八届四中全会报告,彰显在法治思想的精神与理念中。     

跨政府网络与G20的治理模式

本文试图从跨政府网络的角度探讨G20的治理模式,分析G20治理的动力、方式和成果.在世界面对气候变化与金融危机两大挑战之际,作为全球治理核心的G20通过跨政府网络的治理模式,为世界主要经济体的国内规则制定者和政府官员搭建了对话和非正式协调的平台,进而通过与国际组织的互动,实现对相关议题的治理.以跨政府网络为基础而达成的自愿性协议及公报,克服了需要长期谈判及国内批准的条约模式的限制,反映了G20在应对全球问题时的灵活性及迅速调动资源的能力.     

抗人同种异型G1m(3)单克隆抗体研制及特异性鉴定

用快速液相色谱仪从G1m（3）阳性人血浆中纯化IgG1蛋白，用其免疫BALB／C／小鼠．建立了一株分泌抗人G1m（3）单克隆抗体的细胞株（D7E8）。经抑制ELISA，直接ELISA及斑点ELISA分析，证明D7E8抗体具有G1m（3）单一特异性。其培养上清液效价为512倍，并初步应用于法医办案。     

Chemical identification and optimization of the 4-aminophenol colorimetric test for the differentiation between hemp-type and marijuana-type cannabis plant samples

The 4- Aminophenol (4-AP) colorimetric test is a fast, easy-to-use, and cost-effective presumptive assay of cannabis plant material producing different chromophores with THC-rich cannabis (blue color) and with CBD-rich cannabis (pink color). The main drawback of the 4-AP test is a brief observation window where the color rapidly changes to black, limiting the utility of the test. We now report for the first time, the identification of the product chromophores between 4-AP and CBD/THC as well as propose an explanation and a solution for the color degradation of the chromophores. The identification of the chromophores is provided by spectroscopic (UV–Vis), chromatography, and mass spectrometry (TLC and LC-QToF-MS). Oxidation of excess 4-AP (Reagent A) in the presence of NaOH (Reagent B) produces the black color observed for the previously reported 4-AP tests and reported in the literature. The adjustment of reactants concentrations and volumes of 4-AP:THC/CBD to a 1:1 ratio significantly reduces the black oxidation by-product and increases the observation window up to 2 h instead of the previously reported 5–10 min. For the first time, mass spectrometry and chromatography confirmed that the reaction of THC and CBD with 4-AP produced chromophores with m/z (M + H) = 420, consistent with proposed indophenol structures. The TLC method developed confirmed the separation between CBD and THC chromophores. The specificity of the test is also reported, showing false positive results for the presence of THC (blue color) for samples of thyme and oregano. LDA and SIMCA models showed that the optimized 4-AP procedure performs better than the previously reported 4-AP color test.