论改革中国的出入境检查制度

中国现有的出入境检查制度存在不足之处，例如法律欠缺、行政诉讼程序有瑕疵、查控电脑软件有漏洞等。建议通过完善立法、改进电子化管理程度、革新出入境检验技术以及提高执法人员素质等举措来改革中国出入境检查制度，以便落实反恐维稳等保障国家安全的战略对策。     

大学新生思想政治工作探析

本论文针对新时期大学新生的新变化，从大学新生面临着转变学习方式、生活方式中出现的问题以及构建和谐校园的意义方面，阐述了思想政治工作的重要性。在分析大学新生思想政治工作的创新性、复杂性、多层次性基础上，提出了加强大学新生思想政治教育的具体对策。     

我国外资并购中的国家安全审查制度

我国对外资并购的监管在不断变化和调整过程中逐渐形成体系。但现行法律关于国家安全审查的规定相对简单，缺乏系统性和可操作性。国家安全审查有其独立性，行业审查和反垄断审查均不能代替国家安全审查。建议我国成立类似于反垄断委员会的国家安全审查委员会，专门处理外资并购的国家安全审查问题，并从审查对象、审查标准、审查机关、审查程序、救济途径等方面完善国家安全审查制度。     

公安基层派出所“一条龙式”社会矛盾化解机制探索

建设社会矛盾化解机制是一项系统工程,是当前公安基层派出所面临的最为艰巨、复杂和长期的重要任务,这项任务的完成将推进社会矛盾化解工作的顺利开展,将能更好地进行预防和化解各类社会矛盾纠纷,能进一步推动平安和谐社会的构建。     

Detection of genotype recycling fraud in U.S. immigrants

Abstract: Relationship testing laboratories provide genetic evidence to support or refute claims of kinship between U.S. citizen petitioners and potential immigrant beneficiaries. One female beneficiary presented a male amelogenin type and alleles at 15 autosomal loci that were identical to an alleged brother’s. Laboratory records showed that her alleged father had petitioned to have 15 children emigrate from Ghana. The petitioner’s 15 paternity indices exceeded 10⁵, but the children shared only four short tandem repeat (STR) profiles, suggesting fraudulent reuse of genotypes in this alleged pedigree (AP). To determine the extent of this “genotype recycling,” I examined the laboratory’s 555 APs from Ghana and 532 control APs from Nigeria. Seventeen Ghanaian APs (3.1%) but no Nigerian APs showed genotype recycling. Of 90 tested people in the 17 APs, 56 shared identical STR profiles with others in their AP. Of these 56 people, 10 were petitioners with unexpectedly high parentage indices. Seven of 56 had amelogenin types that disagreed with their declared genders. Database searches for identical multilocus genotypes in allegedly different people would best detect this fraud.     

地方蛋鸡群中A亚群禽白血病病毒的分离与全基因组序列分析

利用DF1细胞培养、ELISA和多聚酶链式反应(PCR)从地方蛋鸡群中分离并鉴定了1株A亚群禽白血病病毒(ALV-A),命名为AH10。依据GenBank中已发表的序列分段扩增获得AH10株的全基因序列。序列对比分析发现,AH10株基因全长7 458 bp,其中gp85与国内另一蛋鸡A亚群SDAU09C3株同源性最高,氨基酸同源性达到96.5%。此外,AH10株在非编码区5′-LTR区域存在蛋鸡群A亚群禽白血病所共有的19个碱基的插入突变。研究结果进一步完善了我国地方蛋鸡群中禽白血病的流行病学信息。     

日本学界的《文明论概略》解读

福泽谕吉的《文明论概略》成书于明治八年(1875),该书言简意赅,极具包容性和思想张力,被视为了解日本近代文化必读的经典。日本学界百余年间见仁见智的评价、研究历史已经形成了一个复杂的知识谱系。综述学界的百年阅读史,探寻争论焦点背后潜藏的种种隐喻,可为我国学界的相关研究提供重要的学术参考。     

Enzymatic detection of γ-hydroxybutyrate using aldo-keto reductase 7A2

Gamma-hydroxybutyrate (GHB) is a prescribed medication as well as a drug of abuse. Its detection in various matrices for in-field forensic scientists remains a challenge. We have developed an assay that uses aldo-keto reductase 7A2 (AKR7A2) for the specific determination of GHB in various drinks. AKR7A2 was purified using Ni-affinity chromatography. The Michaelis-Menten constant for the GHB oxidation reaction was 10 mM, and the minimum detection limit was 4 mM. Ethanol was not a substrate for AKR7A2. In a coupled reaction with NADP(+), phenazine methosulfate (PMS), and 2,6-dichlorophenolindophenol, various beverages (orange juice, milk, soda, and numerous alcoholic drinks) containing GHB turned from blue to light yellow. In a second coupled reaction where diaphorase replaced PMS, the presence of GHB also caused the expected change of color in various beers.     

关于《财政与金融教程》的教学思考

在财政与金融课程的教学中,要兼顾财政与金融二者的基本知识和基本理论,领会教学目的要求,把握教材结构体系;要根据教材的重点,运用科学的方法,做到理论联系实际,结合成人教育的特点尝试案例教学,学以致用.     

国产试剂盒Goldeneye~(TM)20A在亲权鉴定中的应用评估

目的评估GoldeneyeTM20A试剂盒在亲权鉴定中的应用价值。方法应用GoldeneyeTM20A试剂盒对289宗亲权鉴定案例中的FTA卡血样本基因组DNA进行PCR扩增,扩增产物用ABI 3130xl遗传分析仪进行毛细管电泳,GeneMapper v3.2和GeneMarker HID软件进行基因分型及统计学分析,并与IdentifilerTM、SinofilerTM、PowerPlex16 3种试剂盒进行比较。结果采用GoldeneyeTM20A试剂盒,累积非父排除概率(CPE)为0.999 999 996,累积个人识别能力(CPD)达0.999 999 999 999 999 999 999 932 44,与目前常用的3种试剂盒相比较,GoldeneyeTM20A试剂盒在不排除的案例中具有更高的CPI值;在排除的案例中具有更多的排除指标。结论国产GoldeneyeTM20A试剂盒在亲权鉴定中有较高的应用价值。