健脾滋肾方对脾肾阳虚型系统性红斑狼疮疾病活动的影响

目的 观察健脾滋肾方对脾肾阳虚型系统性红斑狼疮(systemic lupus erythematosus,SLE)疾病活动的影响.方法 选取SLE患者60例,按照随机数字表法分为对照组和治疗组,每组30例.对照组予醋酸泼尼松片、硫酸羟氯喹片口服.治疗组在对照组基础上加用健脾滋肾方.两组疗程均为12周.比较两组治疗前后中...     

经鼻给药沉香精油对单次长时间应激小鼠PTSD样行为及海马GluN2A、GluN2B表达的影响

目的 评价经鼻给药沉香精油对单次长时间应激（single-prolonged stress,SPS）诱导的小鼠创伤后应激障碍（posttraumatic stress disorder,PTSD）样行为的作用,并从突触的角度探讨其可能的作用机制。方法 通过气相色谱—质谱联用定性分析沉香精油主要成分;将C57BL/6小鼠随机分为正常组、模型组、沉香精油低剂量组、沉香精油高剂量组和阳性药组\[罗利普兰(Rolipram,ROL)\],每组8只。采用行为学实验评价SPS小鼠的PTSD样行为：分别通过恐惧记忆测试实验检测小鼠恐惧凝滞时间,旷场和高架十字迷宫实验评价小鼠的焦虑样行为。Western blot法检测小鼠海马组织N-甲基-D-天冬氨酸受体（N-methyl-D-aspartate receptor,NMDAR）2A亚单位（GluN2A）、2B亚单位（GluN2B）蛋白表达水平。结果 沉香精油样品中共检测到117种挥发性成分。动物实验结果表明,与正常组比较,模型组小鼠表现出强烈的焦虑样行为及恐惧记忆消退异常（P＜0.05）,而沉香精油及阳性药ROL可明显改善SPS小鼠的异常行为（P＜0.05）,并逆转GluN2A、GluN2B的异常表达（P＜0.05）。结论 经鼻给药沉香精油对SPS诱导的小鼠PTSD样行为具有改善作用,其机制可能与调控GluN2A、GluN2B蛋白表达有关。     

金黄色葡萄球菌Fc段受体结合蛋白A基因的克隆与原核表达

为研制葡萄球菌蛋白A(staphylococcal protein A,SPA)的相关免疫试剂盒,设计了1对引物,从金黄色葡萄球菌基因组中扩增出了876bp的细胞壁结合蛋白SPA基因,该基因编码292个氨基酸,包含了E、D、A、B、C 5个IgG结合结构域。构建了原核表达载体pET28a(+)-SPA,经IPTG诱导,表达出分子质量约35.0ku的SPA蛋白,并应用亲和层析柱纯化了SPA蛋白,Western-blot分析表明纯化的SPA蛋白具有生物学活性。     

口蹄疫病毒非结构蛋白3A单克隆抗体的制备和鉴定

以原核表达并纯化的口蹄疫病毒(FMDV)非结构蛋白3A免疫BALB/c小鼠,取其脾细胞与小鼠骨髓瘤细胞SP2/0进行融合,用间接ELISA方法筛选杂交瘤细胞,有限稀释法进行亚克隆,获得3株能稳定分泌抗3A蛋白单抗的杂交瘤细胞,分别命名为3H2、4B1、7F5,并对这3株单抗进行了生物学鉴定。结果显示,单抗3H2和4B1为IgG2a亚型,7F5为IgG2b亚型。单抗7F5针对的表位与3H2和4B1两株单抗的表位不一致;这3株单抗均能够与FMDV特异性结合,且单抗4B1与所有3A相关蛋白均反应。稳定性鉴定结果表明,获得的3株杂交瘤细胞均能稳定分泌单克隆抗体。研究结果为进一步探索3A蛋白的结构和功能以及建立诊断方法奠定了基础。     

同心圆理论

同心圆理论是系列性刑事案件三大侦查理论之一,源于城市结构模型理论,指的是以系列性案件的首发案为圆点、以信息源为依据、以到达系列性案件各子案发案地直线距离为半径,通过几何的方式勾画出犯罪人的活动半径,进而确定犯罪人的居住地和犯罪人的侦查方法。同心圆的原点是系列性案件的首发地,是信息源中元信息的集聚区,反映的是系列性案件中所有个案与首案之间的联系。圆半径的长短,反映了犯罪人对环境熟悉程度的变化,距离越短,其对环境的熟悉程度越高。同心圆理论解决了系列性案件侦查范围划分问题,有积极的实践意义。     

人类命运共同体视域下中国责任建构研究——基于对"中国责任论"的反思与批判

面对百年未有之大变局,中国积极承担国际责任,倡导和构建共赢共享的人类命运共同体.作为中国责任体现的人类命运共同体是对中华优秀传统文化基因的传承,是中国共产党对世界责任的自觉担当,是中华民族实现从站起来、富起来到强起来的历史性飞跃,是实现中华民族伟大复兴的必然要求.在建构人类命运共同体过程中,中国承担和履行人类命运共同体的价值责任、中国的国内责任和国际责任、人类的未来责任.人类命运共同体是对形形色色的"中国责任论"的正面回应和超越,是中国负责任大国的体现和担当.     

They Persist: Parent and Youth Voice in the Age of Trump

In recent decades, parents and youth involved in the child welfare and foster care systems have created myriad ways to have their voices heard and their concerns appreciated, including through collective self‐advocacy efforts. New forms of individual and communal advocacy have emerged, including with supportive professionals, that acknowledge the centrality of parents and youth in every decision being made about their lives and about the systems that control their lives. Nevertheless, studies of youth and parent engagement identify the numerous individual and systemic barriers to meaningful participation and self‐advocacy efforts and the challenges to overcoming those barriers. This essay explores how empowered parents and youth can surmount those barriers with the assistance of their professional allies. Ultimately, this individual and communal engagement will strengthen a family‐oriented child welfare system and a more responsive government in these uncertain times.     

What would John Griffith have made of Jonathan Sumption's Reith Lectures?

In his 2019 Reith Lectures on the rise of law and decline of politics, Jonathan Sumption presents a thesis that, on its face, seems identical to that of J.A.G. Griffith's defence of the political constitution. Given the radical differences in their views on equality, democracy, and redistribution—with Griffith working in the tradition of democratic socialism espoused by the Webbs, Tawney and Laski, and Sumption expressing the libertarian philosophy underpinning Thatcherite policies—this is puzzling. This article sets their views in historical and political context and argues that the similarities are superficial, whereas the differences are profound. It then proceeds to show the weaknesses in Sumption's defence of his thesis.     

Digital behavioral-fingerprint for user attribution in digital forensics: Are we there yet?

the need for a reliable and complementary identifier mechanism in a digital forensic analysis is the focus of this study. Mouse dynamics have been applied in information security studies, particularly, continuous authentication and authorization. However, the method applied in security is void of specific behavioral signature of a user, which inhibits its applicability in digital forensic science. This study investigated the likelihood of the observation of a unique signature from mouse dynamics of a computer user. An initial mouse path model was developed using non-finite automata. Thereafter, a set-theory based adaptive two-stage hash function and a multi-stage rule-based semantic algorithm were developed to observe the feasibility of a unique signature for forensic usage. An experimental process which comprises three existing mouse dynamics datasets were used to evaluate the applicability of the developed mechanism. The result showed a low likelihood of extracting unique behavioral signature which can be used in a user attribution process. Whilst digital forensic readiness mechanism could be a potential approach that can be used to achieve a reliable behavioral biometrics modality, the lack of unique signature presents a limitation. In addition, the result supports the logic that the current state of behavioral biometric modality, particularly mouse dynamics, is not suitable for forensic usage. Hence, the study concluded that whilst mouse dynamics-based behavioral biometrics may be a complementary modality in security studies, more will be required to adopt it as a forensic modality in litigation. Furthermore, the result from this study finds relevance in other human attributional studies such as user identification in recommender systems, e-commerce, and online profiling systems, where the degree of accuracy is not relatively high.     

行政诉权分层保障机制优化研究

基于当事人行政诉权行使理性化程度的不同,可将其区分为理性行使、精明行使、不当行使和恶意行使等四种状态,行政诉讼法实施应当进一步健全行政诉权分层保障机制。对理性行使行政诉权者应当给予有效保障,从科学把握立案登记和审查关系、法官履行释明诉讼类型选择义务、灵活运用行政案件协调化解机制等三个方面进行优化。对精明行使行政诉权者应当给予适度容忍,健全诉讼风险交流机制和诉讼繁简分流机制。对不当行使行政诉权者应当给予必要矫治,完善诚信诉讼的程序规则支撑和诉讼失权制度的精准适用。对恶意行使行政诉权者应当给予严厉制裁,探索差异化立案审查和诉讼失信人信用惩戒机制。行政诉权分层保障机制的优化,对于立基于全民守法的法治社会建设具有重要的促进作用。