Gc亚型检测的复合MS-PCR法及其应用

目的探讨建立Gc亚型检测的复合MS-PCR法及其应用价值。方法根据Gc基因中的2处点突变,设计2对片段相差5bp的等位基因特异性引物和1条公共引物进行复合MS-PCR,分析Gc多态性,并调查武汉地区218例汉族无关个体Gc多态性和鉴定10例亲子关系。结果复合MS-PCR检测的Gc基因型,与AmpliTypePM试剂盒的分型结果一致;武汉地区汉族人群Gc基因的3个常见等位基因Gc1F、Gc1S、Gc2的基因频率分别为0.4816、0.2592、0.2592,观察杂合度(Hobs)、期望杂合度(Hexp)、多态性信息含量(PIC)、个人识别能力(DP)、非父排除率(PE)分别为0.6193、0.6359、0.6253、0.7974、0.3480,基因型分布符合Hardy-Weinberg平衡;真三联体和非真三联体亲子鉴定各5例,前者不排除父子关系,与常规STR分型一致,后者经Gc-MS-PCR分型排除2例。结论建立复合MS-PCR法检测Gc亚型在法医物证鉴定中有实用价值。     

8例利多卡因过敏性休克死的血药浓度分析

目的研究利多卡因过敏性休克死者利多卡因血药浓度及其与死因的关系。方法采用高效液相色谱法(HPLC),分别对使用利多卡因麻醉术中各项指标均正常的8例术中因利多卡因过敏性休克死者的血液和11例顺利完成手术患者的血液进行利多卡因浓度的检测,对比分析两者结果。结果 8例死者利多卡因血药浓度(1．61mg± 0．45mg／L)低于11例正常患者利多卡因血药浓度(2．44mg±0．47mg／L)。结论利多卡因过敏性休克死者血药浓度在正常值范围内,与发生过敏性休克致死无关。     

从非营利组织看事业单位的改革

中国在推进事业单位改革的过程中开始提出和使用非营利组织的概念,正确看待非营利组织现状和在中国公益事业中的作用,让事业单位和民间非营利组织平等发展,甚至相互竞争来促进事业单位提高服务意识和服务质量。使公益性,非营利性得到真正体现,并让改革的成果惠及全体人民。     

中国特色生态文明的理论定位、特质与建构

中国特色生态文明是马克思主义生态理论中国化的最新成果,是中国特色社会主义理论体系的重要组成部分,具有阶段跨越性、历史传承性、人文关怀性以及超越时空性等特点。新时期建设中国特色生态文明,要以科学发展观为指导,以＂两型社会＂建设为载体,以发展生态经济为支撑,以生态制度创新为保障,以健全社会参与机制为突破口。     

Corporatism packaged in pluralist ideology: the case of Slovenia

Corporatism has been an influential doctrine in the Slovenian polity since its beginning. After the onset of democratization in the early 1990s, its influence remains strong. Forms of corporatism are embodied in the National Council as the second chamber of parliament, in the chamber system, the system of social partnership and the RTVS (Public Television of Slovenia) Council. It is also present in certain socio-political priorities such as a higher value being placed on partnership over competition, on fairness over human rights, on community over individualism. Social pluralism has always been a part of Slovenian public life. Political pluralism emerged at the end of the 19th century and was never fully developed. There has always been a strong inclination in the political life of Slovenia to organize around interest groups and editorial boards of various publications, a tendency that reveals a plurality of voices but a general unwillingness to fight for political power. It could also be concluded that the development of pluralism in Slovenia relies heavily on corporatism because of the general lack of liberal foundations.     

SPECIAL NEEDS CHILDREN IN FAMILY COURT CASES

This article presents a conceptual framework for describing cases involving children with special needs that are increasingly being seen in family court. Three categories that represent the most common of such cases include (1) acute, life-threatening medical conditions; (2) chronic developmental disorders; and (3) psychological and behavioral syndromes. After detailing the nature of the disabilities in each category, perspectives from the bench are offered with specific recommendations and general strategies for effective case management. This is followed by a proposal of an innovative conceptual model—the Individualized Parenting Plan (IPP)—that organizes and integrates nine domains essential for a comprehensive parenting plan for special needs children. The article concludes with a list of sixteen strategic guidelines for servicing the families of these special needs children, in their best interests.     

《孟子》荒政思想研究

古代中国自然灾害频发,抗灾救荒成为人们生活中的重要内容之一。《周礼》中出现荒政思想后,先秦诸子对荒政从不同方面加以阐述,文章针对《孟子》中的荒政思想进行论述。首先,《孟子》的"民贵君轻"思想和"仁政"思想是其荒政思想的理论基础;其次,《孟子》的积极预防荒政思想包括:制民之产、劝种备荒、精耕细作、不违农时、薄税赋、兴修水利和保护生态平衡。再次,《孟子》的消极救济荒政思想包括:确定救济主体、移民就粟和移粟就民、赈灾、蠲赋免役、节约、国与国之间的互助和民众互助。     

试论破坏选举罪适用范围之扩展

民主选举是社会主义民主的重要体现和保障，现实中破坏选举的行为层出不穷。而破坏选举罪适用范围上过于狭窄，致使不少严重危害行为置于刑法的射程之外。因此，应将破坏选举罪适用范围扩展到相当范围的政治性选举活动，具体到我国，应包涵基层群众自治组织选举、中国共产党党内选举、人民政治协商会议选举、各级人大代表法律议案表决活动等国家政治生活中重要的选举。     

如何建立健全国有独资公司的法人治理结构

国有独资公司在社会主义市场经济中具有重要作用,但现实运行中其治理结构存在着诸多问题。必须建立和完善以 国资委、董事会、监事会、总经理为中心的公司法人治理结构。设想是:通过增加不同利益主体,实现股权多元化;让利益相关者代 表参与董事会和监事会以实现共同治理;建立董事和监事资格认证制度;设计合理的董事会和监事会成员结构。     

论公民对行政执法的监督

公民对行政执法的监督是社会监督的重要组成部分。目前,公民监督已取得了一定的成就.但公民监督的作用远 没有得到发挥。完善公民监督,应当加大普法宣传的力度,强化对公民监督的法律保障,完善公民监督的有效途 径,实行政务活动公开化,完善听证制度。