宁波甬江水域夏冬季节溺死兔内脏硅藻16SrDNA的鉴定

目的采用PCR法检测夏季7月和冬季12月宁波甬江水域溺死家兔内脏组织中硅藻16SrDNA,评估其在夏季7月和冬季12月溺死鉴定中的应用价值。方法于夏季7月和冬季12月分别选取30只大白兔,总计60只大白兔,随机分为溺死组(n=10)、死后入水组(n=10)、空气栓塞组(n=10)。各组分别提取心、肝、肺、肾组织,应用PCR技术检测上述兔内脏硅藻16SrDNA。结果与死后入水组比较,夏季7月兔溺死组心、肝、肺、肾组织中硅藻16SrDNA检出率明显增高(P<0.05);而冬季12月溺死组仅心、肺组织中检见硅藻16SrDNA,与死后入水组比较无明显差异(P>0.05);夏季7月溺死兔组心、肝、肺、肾组织中硅藻16SrDNA检出率明显高于冬季季12月溺死兔组(P<0.05);夏季7月和冬季12月空气栓塞组心、肝、肺、肾组织中硅藻16SrDNA均未检出。结论宁波甬江水域夏季溺死兔中硅藻16SrDNA检出率高,PCR技术可用于溺死诊断;而冬季硅藻16SrDNA检出率低,运用该技术诊断溺死应慎重。     

30个中低突变Y-STR基因座复合扩增体系的建立

目的建立30个中低突变Y-STR基因座的复合扩增体系,并对其性能进行评估。方法采用六色荧光标记技术,选择30个中低突变率、中高多态性的Y-STR基因座自行设计引物,进行复合扩增和毛细管电泳检测。检测该体系的准确性、特异性、灵敏度和耐受性,并观察其在混合检材的分型情况。结果采用本体系,分型结果准确稳定、特异性好、耐受性强,灵敏度达0.0625ng,混合检材在1:4的比例下,可获准确分型。结论本研究建立的30个Y-STR基因座的复合扩增体系分型结果良好,对于中国人群的法医Y-STR数据库建设和群体遗传学研究具有重要意义。     

发挥桂林统战文化资源优势 促进文化繁荣发展

党的十八大报告旗帜鲜明地提出了建设社会主义文化强国的发展战略。统一战线在社会主义文化建设中有着独特优势和作用。桂林应该充分发挥统战文化资源优势,为推动社会主义文化大发展大繁荣作出应有贡献。     

法医遗传学研究新进展

法医遗传学经过近30年发展,已经形成了一套系统的理论和技术体系,相关技术和方法在实际工作中发挥着重要作用。随着实际应用需求的变化和相关基础学科研究的进展,法医遗传学的研究也出现了新变化。本文针对法医遗传学领域的研究进展和发展趋势进行综述,希望能对相关研究和实践提供参考和借鉴。     

磁珠DNA自动提取系统在法医检验中的应用

磁珠DNA自动提取系统是将磁珠分离技术与DNA自动提取工作站结合运用,以达到高效提取DNA的目的,它具有操作时间短、准确性高、检材用量少等特点,特别适用于大批量生物检材的快速提取,是目前主流的DNA自动化提取方法。本文对磁珠DNA自动提取系统的基本组成、工作原理、操作流程及其在法医检验领域中的应用等进行了综述,并对该系统的发展前景进行了展望。     

Examining the Use of Informal Networks by NGOs in Azerbaijan and Georgia

Abstract

The aim of this article is to investigate whether and to what extent changes in the structural preconditions for volunteering have had an effect on the relative importance of ‘classic predictors’ of volunteering within two areas: (1) socio-economic and status variables that function as resources for civic engagement and (2) value orientations that guide volunteering. Arguably, upward changes in the level of human capital, such as education, or changes in values that put more emphasis on, for example, social responsibility, could be expected to lead to an increase in the relative importance of these variables because the groups who possess such resources or express such attitudes would be more likely to enter into the volunteer labour force. If this is the case, then we would expect to see increasing differences in the likelihood of volunteering between high- and low-status groups. On the other hand, it could be argued that it is the distribution and diffusion of such resources and values that matter. If, for instance, education has become more widespread and covers larger proportions of the population, it is also possible that the relative importance of education has diminished. In this case, we would see a mainstreaming effect on volunteering because this activity becomes more normal, independent of class and status. Finally, it is also possible that volunteering is relatively resistant to wider structural changes in society or at least experience a time lag in the effect of such changes. Based on an empirical analysis of Danish survey data from 1990 to 2008, we argue that the idea of a mainstreaming effect should be taken seriously.     

From the Balconies to the Barricades,and Back? Trajectories of Participation in Contentious Politics

Scholars have long been interested in explaining why some individuals engage in civil society through acts of protest while others do not. However, what happens after individuals are involved? Using a nationally representative panel data set that follows Americans from 1965 until 1997, I show that almost half of participants either engage in ‘individual abeyance’, moving in and out of engagement over time, or disengage. I examine the role of socio-political orientations, resources, biography or life-course factors, and group affiliation in predicting patterns of civil society participation over time. Past work suggests that persistent activists differ from those who disengage due to the formers’ particular socio-political orientations. However, I show that there are no significant differences in these orientations between those who persist and those who do not. Instead, biographical changes and engagement in political groups are the most important factors predicting persistent participation over time.     

当前我国教育资源配置的公平与效率

我国现阶段教育资源配置之所以作为问题而存在,主要原因是教育资源的有限性与稀缺性,其不能够为社会所有成员提供有效的供给,由此引发需求与供给之间的矛盾。这些问题主要是存在以下几个方面：教育资源配置过程中存在的公平与效率对比不均问题;教育资源配置结果中存在的公平与效率对比不均问题。要解决这些问题,就需要多方面地思考,教育资源配置中公平与效率的基本含义及其关系首先要搞清楚,在此基础上,作为制度供给主体的政府要在法律、教师待遇、学生待遇等各个方面予以支持。我国教育资源配置的公平与效率合理比例不仅是教育发展的要求,更是和谐社会发展的要求。     

The spatial division of labour in Nepal

This article examines how economic activity and market participation are distributed across space. Applying a nonparametric von Thünen model to Nepalese data, we uncover a strong spatial division of labour. Non-farm employment is concentrated in and around cities while agricultural wage employment dominates villages located further away. Vegetables are produced near urban centres; paddy and commercial crops are more important at intermediate distances. Isolated villages revert to self-subsistence. Findings are consistent with the von Thünen model of concentric specialisation, corrected to account for city size. Spatial division of labour is closely related to factor endowments and household characteristics, especially at the local level.     

Spectrofluorimetric and Spectrophotometric Analysis of Two Analgesic Drugs in Pharmaceutical Formulations and Biological Fluids

Simple, reliable, sensitive, and accurate spectrofluorimetric and spectrophotometric methods were proposed for the determination of two selected analgesic drugs, namely tramadol and morphine, in pharmaceuticals and biological fluids. The proposed methods were based on the oxidation of the studied drugs by Cerium (Ce)IV in an acidic medium. The spectrofluorimetric method is based on measuring the relative fluorescence intensity of Ce(III) arising from Ce(IV) at 350 nm with an excitation wavelength at 250 nm. The spectrophotometric method involves on addition of a known excess of Ce(IV) to the studied drugs in an acid medium, followed by the determination of residual Ce(IV) by reacting with a fixed amount of methyl orange and measuring the absorbance at 510 nm. Different variables affecting the reaction conditions, such as the concentrations of Ce(IV), type and concentration of the acid medium, reaction time, temperature, and the diluting solvents, were carefully studied and optimized.