不同培养液对牛孤雌生殖胚胎和体细胞核移植胚胎体外发育的影响

采用体外成熟的牛卵母细胞 ,经 5 μmol/LIonomycin 5min和 2mmol/L 6 DMAP 5 μg/mLCCB 4h激活 ,分别在TALP M 199(TM ,1∶2 )、BMOC M 199(BM ,1∶2 )和CR1aa培养液内培养 ,2 4h和 3 6h各组卵母细胞的卵裂率分别为5 6.4%、5 5 .6%、5 3 .6%和 72 .0 %、75 .9%、79.0 % ,无显著差异 (P >0 .0 5 ) ;培养 8d后囊胚发育率分别为 18.2 %、2 1.4%和 2 9.3 % ,CR1aa内培养孤雌生殖胚的囊胚发育率显著高于TM (P <0 .0 1)。皮肤成纤维细胞构建的重组胚经同法激活后 ,分别在TM、BM和CR1aa内培养 ,2 4h和 3 6hBM和CR1aa内重组胚的卵裂率显著高于TM (4 9.7%、49.8%比 2 0 .5 % ;74.1%、73 .2 %比 5 0 .0 % ) (P <0 .0 1) ;虽然培养 8d后各组胚胎的囊胚发育率无显著差异 ,但CR1aa内重组胚的囊胚发育率稍高 (12 .3 %比 9.8%、11.5 % )。结果表明 ,CR1aa更适合孤雌生殖和体细胞核移植胚胎的体外培养。     

Key longitudinal-experimental studies in criminology

Following the tradition of Joan McCord's work, this paper reviews longitudinal-experimental studies in criminology with community samples of at least 100 persons, follow-up periods of at least 5 years, personal interviews, and measures of offending. The main advantages of such studies are in investigating both the natural history of development (including the effects of risk/protective factors and life events) and the impact of interventions on offending. This paper also reviews advantages and problems of prospective longitudinal surveys, randomized experiments, and longitudinal-experimental studies in criminology. Four key longitudinal-experimental studies were conducted by Joan McCord, Richard Tremblay, Lawrence Schweinhart and David Olds. Other studies have been conducted, or are currently under way, but no criminological study has yet been published with several years of personal data on participants both before and after an intervention.Joan McCord Award Lecture given at the American Society of Criminology, Toronto, Canada.     

Preventive Care for Patients with Coronary Heart Disease

Preventive care and patients' health behaviors cah have dramatic impacts on the course of coronary heart disease (CHD). Although the importance of preventive care is accepted by most health care providers, studies consistently indicate that the delivery of these services is frequently inadequate. This article reviews the literature on the development of strategies to provide preventive care. Its aim is to shed light on potential methods for improving preventive service delivery to patients with CHD. The thesis is that through greater attention to the role of information technology and creative strategies for improving patient outreach, barriers that have been encountered in the provision of preventive care may be surmountable. In particular, recent advances involving computer systems to remind clinicians to perform preventive procedures and more aggressive telephone contact with patients between ambulatory care visits may improve the care of patients with CHD. Future work is needed to refine these strategies and to identify the types of CHD patients who are most likely to benefit from receiving them.     

发展中国家对现代海洋法发展的贡献

海洋法是国际法中新兴而又古老的法律部门,在20世纪30年代以前,只有少数国家能够在各个方面利用和开发海洋,因此,海洋法就成了他们掠夺资源、行使海洋霸权的工具。20世纪30年代以后,尤其是二战结束之后,随着广大发展中国家利用和开发海洋的能力和意识的不断增强,提出了许多顺应潮流的海洋法理论。为了使这些理论得以落实,广大发展中国家联合起来同少数发达国家展开了激烈的斗争,从而为现代海洋法的发展做出了重大贡献。     

浅议邓小平的民主法治思想与人本发展观

崇尚法治,反对人治是邓小平法治思想的基础和核心,民主法治思想与人本发展观的完善是邓小平理论的结晶,它的立法、执法、守法等基本思路对维护社会主义市场经济的新秩序,具有广泛深远的意义。     

The Politics of Universalisation: Establishing National Health Services in Southern Europe

Italy, Portugal, Greece and Spain have enacted reform laws during the last 20 years with the intention of turning their health insurance systems into national health services. Universalisation of access to public health care was at the centre of the political debates which led to the passing of the reform laws. This article analyses the policy-making processes that allowed for such institutional change, as well as achievements and shortcomings of the implementation processes that followed. The analysis draws on the insights of the actor-centred neo-institutionalist approach in the policy sciences.     

民族地区高等教育改革与发展的策略思考

民族地区高等教育面临着良好的发展机遇,也潜藏危机和挑战.必须进一步调整民族地区高等教育的改革与发展思路.要做到正确定位,确立特色;加大投入力度与充分挖掘自身潜力;以人为本;办学模式的创新;内联外引,吸纳先进的办学理念;全面提高教育质量.     

对市级党校社会培训跨越发展的理性思考

推动市级党校社会培训跨越发展是新形势下一项重要的现实课题。市级党校在开展社会培训时,不能把追求经济效益作为首要任务,而应把社会效益、围绕中心服务大局放在第一位,通过争取政策、建章立制、队伍建设、打造团队、转型增效、提炼精品、塑造品牌、深化合作等路径,推动社会培训实现跨越发展。     

我党反腐倡廉建设90年历程的回顾与思考

中国共产党自建立以来,经历了各个历史时期反腐、拒腐、防腐、治腐的光辉历程,积累了宝贵经验。在大量史实基础上,从七个方面总结概括,指出今后要以科学发展观为指导,提高制度执行力,加强党员干部教育,强化人民监督力度,以改革创新精神继续做好反腐倡廉工作。     

促进大理经济社会持续快速协调发展的思考

全面、准确、积极地贯彻落实中央宏观调控政策,必须从大理实际出发,研究治本之策,谋划长久之计,抓住机遇,与时俱进,努力促进大理经济社会持续快速协调发展.