超声辅助固相萃取-HPLC法测定不同地区缴获鸦片样品中生物碱

目的本文建立了超声辅助固相萃取-HPLC法定量测定缴获鸦片样品中吗啡、可待因、蒂巴因、罂粟碱、那可汀5种生物碱并同时检出6种未知化合物的方法。采用主成分分析法区分不同地区缴获的鸦片样品。方法采用超声辅助固相萃取法提取生物碱,XDB-C18（5μm×4.6mm×250mm）色谱柱,流动相为10mM的1-庚烷磺酸钠（pH 3.2）和乙腈,梯度洗脱,紫外检测器检测。结果该方法的定量限为0.58~2.78 mg/kg。添加水平在0.2～1.5mg/mL范围内,平均加标回收率为81.0%～99.2%,相对标准偏差为1.6%～5.0%。结论本方法重现性好、定量准确,满足定量检测鸦片中生物碱含量并对不同产地鸦片进行分类的需要。     

行政裁量基准的技术范式研究

"裁量基准运动"在实践中开展得如火如荼,理论研究也日渐深入,在防止裁量滥用、构建裁量治理模式方面确实起到了很大的作用。但是,裁量基准能否在实践中真正发挥自己应有的作用和效能,在很大程度上还取决于其制定过程中能否合理地考量各种技术因素。因此,将客观的技术标准植入主观的裁量过程,对影响裁量基准制定的因素予以量化,规划研究它们对于合理估量裁量因素的正义层级,以求改进裁量基准制定技术、保证个案正义将大有裨益。     

Soil sample metagenome NGS data management for forensic investigation

Soil analysis is a valuable resource in forensic investigation. Classical forensic soil analysis involves examination of its physical characteristics and chemical composition, such as soil type, colour, particle size, shape, pH, elemental, mineral and organic content. However the limited variability of these parameters is not always allowing adequate discrimination between soil samples. As soil supports extreme diversity of microorganisms and eukaryotic communities, microbiological approaches have been proposed. Several molecular approaches for microbial DNA profiling are available; however there is a lack of published data of implementation of the next generation sequencing (NGS) approaches for forensic soil analysis.The aim of the current study was elaboration of criteria for soil metagenome data management and database searching. We used our previously sequenced collection of 11 samples collected from different environments (forests, fields, grasslands, urban park) with different flora. The single sample collection includes 9 soil samples per one sampling area (30 m × 30 m) spaced by 15 m. In the current study we concentrated mainly on 18S rRNA gene V2-V3 region for fungi however SSU rRNA region for arbuscular mycorrhizal (AMF) fungi and V2-V3 hypervariable region of 16S rRNA gene for bacterial communities were taken into account. The sequencing was performed by Roche/454 platform. For data analysis OTU based approach on mothur software and NCBI BLASTN search were used. NCBI BLASTN analysis revealed altogether 2983 AMF matches and 8997 18S matches as well as 25477 OTUs (16S) were determined. Several data filtration approaches were used for data management. We found that 18S marker results could be used to create and run a filtered database that is computationally much more efficient and flexible. Our results have broad impact; however more samples have to be analysed, additional studies performed and cooperation between soil scientists and forensic scientists is required to be able to implement these novel techniques into the routine forensic practice.     

Multiplex high resolution melt (HRM) messenger RNA profiling assays for body fluid identification

Traditional body fluid identification methods use a variety of technologically diverse techniques that do not permit the identification of all body fluids. Definitive identification of the biological material present can be crucial to a fuller understanding of the circumstances pertaining to a crime. Thus definitive molecular based strategies for the conclusive identification of forensically relevant biological fluids need to be developed. Messenger (mRNA) profiling is an example of such a molecular based approach.Current mRNA body fluid identification assays typically involve either capillary electrophoresis (CE) or quantitative RT-PCR (qRT-PCR) platforms, each with its own limitations. Both platforms require the use of expensive fluorescently labeled primers or probes. CE-based assays require separate amplification and detection steps thus increasing the time required for analysis. For qRT-PCR assays, only 3 or 4 markers can be included in a single reaction since each requires a different fluorescent dye. To simplify mRNA profiling assays and to reduce the time and cost of analysis, we have developed multiplex high resolution melt (HRM) assays that provide an identification of all forensically relevant biological fluids and tissues.     

The efficiency of Y-chromosome markers in forensic trace analysis and their inclusion in the Austrian National DNA Database

Y-STR markers are a valuable tool in the analysis of biological traces in which a mixture of male and female trace material is to be expected. It is possible to generate a Y-chromosome DNA profile, even if all the prior sperm tests are negative and no sign of any male component is found in amelogenin. In 38 of a total of 239 sexual offences a perpetrator trace was identified solely using Y-STR analysis. Based on these findings, the Austrian National DNA Database was expanded to include Y-STRs in 2012 with the primary objective to identify serial sexual offences.     

Book reviews

Making Sense in Law: Linguistic, Psychological and Semiotic Perspectives, BERNARD S. JACKSON Liverpool: Deborah Charles Publications 1995

Culture And Sexual Risk: Anthropological Perspectives On Aids, HAN T. BRUMMELHUIS &; GILBERT HERDT (Eds) Amsterdam: Gordon and Breach Publishers, 1995

Virtual Geography, MCKENZIE WARK, Bloomington: Indiana University Press, 1994     

The Defining Elements of Advocacy Coalitions: Continuing the Search for Explanations for Coordination and Coalition Structures

As the theoretical and practical interest in policy networks increases, so does the need for further research into how, and based on what rationales, actors within a policy subsystem engage in interorganizational collective action and form political coalitions. The aim of this paper is to continue the search for explanations for coordination and coalition structures in the setting of Swedish carnivore policy. Based on the Advocacy Coalition Framework (ACF) and a previous case study within the same policy subsystem, the study investigates a set of hypotheses regarding actors' coordinating behavior and the defining elements of coalitions. The empirical analysis indicates, in support of the ACF, that perceived belief correspondence is a better predictor of coordination than perceived influence. Moreover, the explanatory power of empirical policy core beliefs in general, and normative policy core beliefs in particular, is further reinforced, while deep core beliefs seemingly do not influence coalition structure. The relevance of more shallow beliefs for coalition formation cannot be dismissed and therefore calls for additional research.     

Solitary Sunnah and Its Legislative Value Among the Muslim Jurists： Readings of Selected Examples

The science of hadith authentication and classification （ulum al-hadith） has been developed by Muslim scholars mostly based on critical analysis of the texts and chains of the reports （ahadith） that are attributed to Prophet Muhammad （s.a.w.）. Though this science is basically designed to identify the authentic reports which are truly the words, actions or tacit approvals of the Prophet （s.a.w.）, however, it has left its implications onfiqh （legal） opinions among the Muslim jurists （fuqaha）, who left contrasting legal opinions on certain issues offiqh, some of which are as a result of their differences on hadith classification, particularly classifications of hadith into solitary and recurrent reports. This paper uses analytical method, and intends to address the implications of hadith authentication and classification methods on the legislative power of the sunnah, with particular emphasis on the solitary sunnah and its legislative value among the Muslim jurists.     

海洋刑法学的提出与国际海上犯罪的立法规制

随着人类社会对海洋资源的开发与利用,涉海领域的犯罪将愈演愈烈,直接危及作为海洋大国的中国的经济健康发展,加之我国刑事立法与刑事法学的严重滞后,迫切需要加强海洋刑法学的研究;海洋刑法学的研究对象是国际海上犯罪,其重点在于"国际"、"海上"犯罪,较之一般犯罪在法律适用、行为方式、行为时空等方面存在明显区隔;对于国际条约中所涉及的国际海上犯罪在国内的适用应采取转化的操作模式,可考虑在刑法典中独立设置章节对其进行规定。     

胡锦涛的团结观论析

胡锦涛的团结观建构在马克思主义团结观的基础之上,是对毛泽东、邓小平、江泽民团结思想的继承和发展,是凝聚人心、完成执政使命的必然。从"斗争思维"向"合作思维"的转变是胡锦涛团结观的逻辑前提,胡锦涛重视团结意识的强化,主张"建设和谐社会",用中国特色社会主义伟大事业凝聚人心、增进共识、促进团结。当下,胡锦涛的团结观有着重要的启示。