30个中低突变Y-STR基因座复合扩增体系的建立

目的建立30个中低突变Y-STR基因座的复合扩增体系,并对其性能进行评估。方法采用六色荧光标记技术,选择30个中低突变率、中高多态性的Y-STR基因座自行设计引物,进行复合扩增和毛细管电泳检测。检测该体系的准确性、特异性、灵敏度和耐受性,并观察其在混合检材的分型情况。结果采用本体系,分型结果准确稳定、特异性好、耐受性强,灵敏度达0.0625ng,混合检材在1:4的比例下,可获准确分型。结论本研究建立的30个Y-STR基因座的复合扩增体系分型结果良好,对于中国人群的法医Y-STR数据库建设和群体遗传学研究具有重要意义。     

The Politics of Infrastructural Projects: A Case for Evidence-Based Policymaking

Many developing countries currently face tightening fiscal constraints as a result of the global financial crisis and associated reduction in both credit and demand. Policymarkers therefore have less fiscal space within which to undertake projects, so the opportunity cost of any misallocation of resources will be correspondingly higher. There is therefore an increasing need to rely more on evidence-based policymaking (EBPM). The is particularly so for large infrastructural projects, as these tend to be costly, and especially for transportation projects as these are particularly prone to result in sub-optimal outcomes. This paper examines the policy process for the implementation of the Jamaican Highway 2000, one of the largest and most costly infrastructure projects in Jamaica in recent years. A number of primary and secondary data sources were explored to ascertain the extent to which the decision to implement the project reflects a case for evidence-based decision-making in practice. This made it clear that the decision was taken at least partly on political grounds, largely in the absence of any supportive evidence, and on the basis of over-optimistic and unrealistic assumptions, and that this has created a number of serious, long-term challenges for Jamaica.     

影响我国碳金融发展的原因分析

与西方发达国家较为完善的碳金融发展相比,我国碳金融发展相对比较滞后,造成这种状况的原因主要有：一是对碳金融的认识有待深化;二是碳金融的综合配套政策不完备;三是碳金融市场分割现象严重;四是中介服务机构发育不完善。对这些原因进行深入分析,有利于排除碳金融发展的障碍,开创我国碳金融发展的光明前景。     

Turning Housing Into Driving: Parking Requirements and Density in Los Angeles and New York

This article examines the idea that residential minimum parking requirements are associated with lower housing and population densities and higher vehicle densities (residential vehicles per square mile). Cities frequently use minimum parking requirements to manage traffic, but parking requirements accommodate vehicles, suggesting they should lead to more driving and congestion rather than less. If parking requirements reduce congestion, they likely do so not by reducing the number of vehicles in an area but by reducing the densities of housing and people. We support this idea by comparing the Los Angeles and New York urbanized areas. We show that differences in housing, vehicle, and population densities across and within these urbanized areas are closely correlated with differences in the share of housing units that include parking, and that the share of housing units that include parking is in turn correlated with the stringency of parking requirements. Compared with Los Angeles, New York shifts less of the cost of driving into its housing market. We further show that within New York City, a 10% increase in minimum parking requirements is associated with a 5% increase in vehicles per square mile, a 4% increase in vehicles per person, and a 6% reduction in both population density and housing density. These relationships remain even after controlling for street layout and proximity to the subway.     

俄罗斯远东地区运输系统现代化改造问题研究

交通运输系统将俄罗斯远东地区内部各主体之间以及与俄罗斯其他地区之间联系起来,是远东地区融入俄罗斯统一经济空间、“确保领土完整和国家统一”的必要硬件条件。也是俄罗斯与亚太地区国家往来的重要基础。面对蓬勃发展的亚太地区,俄罗斯希望通过远东地区实现与亚太地区的经济一体化。为此,俄罗斯着手大力开发远东地区,前不久成立了远东发展部。俄罗斯对该地区交通运输系统进行现代化改造,是改变当地经济硬环境的一个重要举措。     

The Gautrain project in South Africa: a cautionary tale

This article analyses the recently completed Gautrain Rapid Rail Project in the province of Gauteng, South Africa. The Gautrain is the continent's first high-speed rail project, and constitutes an important case in transportation megaproject planning. The article discusses the potential merits and limitations of the project, and ultimately argues that several key problems with the Gautrain should provide readers with a cautionary tale regarding such endeavours. Benefits to the project, such as reduced traffic congestion and job creation, must be weighed against the social and political implications of the project. Thus, the article discusses the following potential problems with the project: it may deepen mobility-related exclusion in the province; it prioritizes wealthy, as opposed to poor, citizens in the allocation of public funds; costs for the project have escalated considerably from initial estimates; and other options for a more effective and integrated transportation plan were not sufficiently considered. This case study conforms to what scholars call the megaprojects paradox in significant ways, and suggests that more rigorous and transparent debate should precede the approval of such projects.     

浅析影响碳素墨水显现胶带指纹的若干因素

为提高胶带粘面指纹的显现质量,便于后期针对该指纹的检验鉴定,通过对胶带粘面指纹显现过程中的各个环节进行测试、对比,可以寻找出一些影响显现效果的因素。经过实验发现,在胶带剥离阶段及对胶带的浸泡、显现阶段,各种不同的实验材质、环境、手法均会对指纹显现效果产生影响。     

Validation of the Short Amplicon Multiplex Q8 Including the German DNA Database Systems*

Abstract: We have developed a concept to enable the analyzing of degraded stains with limited DNA template quantity. Therefore we have constructed a short tandem repeat (STR) multiplex including the German DNA database systems (Q8). The amplicon lengths are smaller than 280 bp. For the validation of Q8 over 50 degraded samples were investigated. Amplifications were performed with “low copy number” PCR, the number of PCR cycles was increased to 33 and the reaction volume was decreased to 12.5 μL. Compared with the MPX2 and Nonaplex kit, the average success rate was increased using the Q8 kit by approximately 20% and 30%, respectively. The efficiency of a sensitive STR multiplex with reduced amplicon lengths was confirmed in comparing the success rates of Q8 for typing degraded samples and samples with limited amount of DNA template while partial profiles were observed with the majority of the samples using commercially available kits.     

Diesel fumes do kill: a case of fatal carbon monoxide poisoning directly attributed to diesel fuel exhaust with a 10-year retrospective case and literature review*

While it is known that diesel fuel combustion engines produce much lower concentrations of carbon monoxide (CO) than gasoline engines, these emissions could certainly generate lethal ambient concentrations given a sufficient amount of time in an enclosed space and under suitable environmental conditions. The authors report a case of CO poisoning which was initially referred for autopsy as a presumed natural death of a truck driver found in the secure cab of a running diesel tractor trailer truck. Completion of the preliminary investigation ascribed death to complications of ischemic heart disease (IHD), pending toxicological analysis that included quantification of CO. When the toxicology results showed lethal blood COHbg, the cause of death was re-certified as CO intoxication secondary to inhalation of (diesel) vehicular exhaust fumes. Because of the unique source of fatal CO intoxication in this case, the contributory IHD and the possible contaminants in the putrefied blood, a 10-year retrospective review was conducted on all nonfire related CO deaths autopsied (n = 94) at the Office of the Chief Medical Examiner in Louisville, KY from 1994 to 2003. For validation of the COHbg detection method used by the Kentucky Office of Forensic Toxicology (KYOFT), blood samples from these cases along with controls were submitted to three laboratories using various analytical methods yielding no statistically significant differences. Lastly, an extensive literature review produced no scientifically reported cases of fatal CO poisoning attributed to diesel fuel exhaust.