猪生殖与呼吸综合征病毒GP5蛋白抗原表位基因的表达及其表达产物的抗原性

为了确定猪生殖与呼吸综合征病毒GP5蛋白A、B抗原表位之间的相互关系和对免疫的影响,应用PCR方法扩增出A、B抗原表位基因并分别克隆于原核和真核表达载体中。原核重组质粒表达的重组蛋白经Western-blotting检测证明,该重组蛋白具有良好的抗原性。将重组蛋白和真核表达质粒免疫小鼠,前者可诱导产生针对PRRSV的特异性ELISA抗体,但是此抗体没有中和活性;后者不能诱导免疫小鼠产生PRRSV特异性抗体。结果表明,中和抗体的产生不但与A、B表位的空间构象有很大的关系,如果蛋白质分子质量过小也不能诱导机体产生有效的免疫反应。     

A NEW LOOK AT AN OLD ISSUE:

In 1997, the Office of Child Support Enforcement initiated the State Child Access and Visitation (AV) Grant Program, which involves annual awards of $10 million to states to promote the development of programs to alleviate access problems. Telephone interviews with 970 parents who used mediation, parent education, and supervised visitation programs funded by AV grants in nine states revealed that the programs are reaching diverse groups of parents including many low-income, non-White, and unmarried parents who receive no other type of access assistance. The programs also appear to be achieving the major objectives posited for them by the federal government. One-third to one-half of noncustodial parents in every program type reported that parent–child contact increased following program participation, with supervised visitation users who typically had the lowest levels of parent–child contact reporting a significant increase in the number of days of contact. A review of child support records for 173 program users in three states revealed that child support payments increased among participants following program participation, especially for never-married parents who paid a significantly higher proportion of what they owed. These findings are similar to results reported in a five-state study of mediation programs funded by AV grants that was conducted by the Office of the Inspector General. However, both studies have some serious limitations, including low response rates and the absence of a nontreatment comparison group.     

中日比较文学研究之现状与未来

关于中日比较文学研究,目前在国内作为热门学科发展得非常快。然而,由于该学科是在两个不同的平台上各自发展,相互之间缺少有效的统合与交流,致使中日比较文学研究出现了一些十分重大的方向性问题与方法问题。以日本文学研究在中国、中日比较文学研究之现状、中日比较文学之未来———方法论为议进行阐述,可以对中日比较文学研究之现状与未来有较为全面的了解。     

多学科、兼顾历史与现状、教学相长：南洋研究院华人研究特点与存在问题（2000-2006）

南洋研究院的华侨华人研究体现了多学科、兼顾历史与现状、教学相长的特色。除了经济、历史、政治学(国际关系)等主要学科外,南洋院师生越来越多地借重民族学、宗教学、金融学、人口学、语言学、管理学、图书及版本学等学科理论与方法研究华侨华人。在侧重华侨华人现状研究时,仍继续关注华人史研究。在相关学位课程带动下,硕博生的学位论文研究日益成为南洋院华侨华人研究的重要组成部分。就总体而言,南洋院的华侨华人研究仍呈现创新能力弱、文献单薄、低水平重复等问题,这既是国内文科重量化考核导向的结果,也与研究人员浮躁的学术态度相关。     

Participation,education, and pluralism: towards a new extension ethic

The concern driving current debate on agricultural extension is increasingly that of how to help farmers learn how to deal with the complex world around them responsibly and profitably, in such a way that the extension worker is ancillary. This article seeks to deconstruct and provide a more reasoned assessment of agricultural extension services through a reflection on development paradigms, adult education, individual empowerment, and institutional pluralism. By calling into question the underlying ethical dimensions of agricultural extension, it is possible to develop an alternative paradigm and thereby generate new insights into it. The article concludes that the raison d'être of agricultural extension today must be to create an ethical basis which ensures that extension practices are more inclusive and thus responsive to the needs of farmers and other rural populations, integrating individual expectations into the wider socio-economic, cultural, political, and geographical environment.     

简论区域经济宏观调控的几个关系

协调区域经济发展是政府的重要职责 ,在缩小区域发展差距问题上 ,必须充分发挥政府的调控作用 ,通过市场调节与政府宏观调控结合 ,促使区域经济发展差距缩小和协调发展。在区域经济发展宏观调控中 ,中央政府调控与地方政府调控各自发挥着它们的作用 ,它们彼此是不能互相替代的     

比较按揭法律制度

近年来,在我国房地产业发展的过程中,引入了香港地区的商品房预售按揭贷款制度我国引入后的按揭制度与香港按揭制度因法律制度的根本不同而存在区别,应将其界定为以一般债权为标的物的权利质押的方式,并将其与抵押、让与担保等相关法津制度进行区别,进一步完善我国的在建工程权利质押制度     

民营经济发展给地方政府管理带来的挑战——以吉林省为例

民营经济是推动政府体制改革的重要力量,而政府管理创新则是推动民营经济蓬勃发展的重要保证。改革开放以来,吉林省民营经济创下许多佳绩,但是同全国民营经济发展状况相比,不论是经济总量还是速度水平都存在较大的差距,还有许多因素制约着民营经济的发展。只有创新地方政府的管理,转变政府职能,改革政府传统的行政管理方式,积极培育和引进民营经济的创业主体,创造条件解决民营经济在发展中的资源要素问题,才能促进民营经济的发展。     

关于创新工会宣教工作的思考

在新形势、新任务的要求下,工会宣教工作面临着与时俱进创新发展的问题。目前,创新工会宣教工作,就是要把围绕奥运开展的各项工作作为一项育人工程、系统工程、精品工程,从观念、思路、内容、形式、机制、制度等方面实现创新发展。     

2004年俄罗斯油气工业的现状、问题与前景

2004年是俄罗斯油气工业快速发展的一年,石油和天然气的产量、出口量及加工量都保持了强劲的增长势头。但是,尽管2004年俄罗斯油气工业的整体形势比较令人满意,可油气资源基地的滞后发展和出口管线问题在一定程度上制约了俄罗斯油气产量的增长。因此,寻找、勘探、开发新油气田,加快油气运输基础设施建设的步伐是俄罗斯油气工业未来的发展方向。