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81.
Mutations might challenge the paternity index calculation in forensic identification. While many studies have focussed on the autosomal short tandem repeats (A-STR), the mutation status of sex chromosomes and single nucleotide polymorphism (SNP) remain blank. Next generation sequencing (NGS), known as high throughput and large sequence polymorphism, is a promising tool for forensic genetics. To describe the mutation landscapes in the paternity cases with genetic inconsistencies, a total of 63 parentage confirmed paternity cases contained at least one mismatched locus have been collected. The mutations were subsequently evaluated using Verogen’s MPS ForenSeqTM DNA Signature Kit and a microsatellite instability (MSI) detection kit. The result showed 98.41% (62/63) of the cases had no additional autosomal mutations even when the number of A-STRs increased to 27. As for the sex chromosomes, about 11.11% (7/63) of the cases exhibited either X-STR or Y-STR mutations. D2S1338, FGA and Penta E were the most frequent altered STRs, which suggested they might be the mutation hotspots. In addition, a male with sex chromosome abnormality was observed accidently, whose genotype might be 47, XXY, rather than MSI. Nearly 56.90% of the STR loci possessed isoalleles, which might result in higher STR polymorphisms. No Mendelian incompatibility was detected among the SNP markers, which indicated that SNP was a more reliable genetic marker in the genetic-inconsistent paternity cases.Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.2009631 . 相似文献
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83.
Suspects in shooting investigations in Chicago are routinely transported in department vehicles and detained in department facilities prior to gunshot residue (GSR) evidence collection. The GSR test results are used to associate the suspect with primary exposure to GSR. The potential for these vehicles and facilities being sources of secondary GSR contamination needed to be determined. A total of 201 samples were collected from randomly selected vehicles and detention facilities. The sampling collected trace materials from surfaces that suspects' hands may contact during the arrest process. These samples were examined for the presence of GSR particles using scanning electron microscopy. Upon completion of the automated analysis, those particles that met an initial GSR screening criterion were relocated and reanalyzed. The locations where GSR particles were recovered allowed us to make recommendations to the Chicago Police Department with regard to transporting and detaining these suspects. The low number of GSR particles recovered suggests that the potential for secondary contamination, although present, is relatively low. 相似文献
84.
Andrzej Olejniczak M.Sc. Aleksandra W. Cyganiuk M.Sc. Jerzy P. Lukaszewicz Ph.D. 《Journal of forensic sciences》2010,55(3):747-752
Abstract: This article extends the application of 1,2,4‐triazine‐based chromogenic reagents to the detection of nonferrous metal traces left on contact with canvas and human skin. The possibility of detection of iron traces resulting from contact with objects made of stainless steel was investigated as well. Additionally, the ability of triazines to form chromatic complexes with Zn2+, Ni2+, Co2+, Cu2+, Cr3+, and Al3+ ions was studied spectrophotometrically. Molar absorption coefficients, ranging from 8.8 to 29.9 × 103/M/cm, provide high sensitivity of 1,2,4‐triazines toward nonferrous ions, thus, enabling the detection at concentrations as low as a few μM. The method was sensitive enough to detect traces resulting from a 1‐min contact with a stainless steel made object, which is commonly considered as a corrosion‐resistant material. The amounts of metal ions transferred to the skin after a 2‐min contact with objects made of brass, zinc, and copper were sufficient to develop chromatic imprints. 相似文献
85.
86.
《Forensic Science International: Genetics Supplement Series》2013,4(1):e27-e28
The Ge.F.I. (the Italian Speaking Working Group of ISFG) has launched the first DNA Proficiency Test in 2012. The aim is to increase the external quality controls in forensic genetics in Italy and to drive to the standardizations of methods within the laboratories. Reference and mixed stains typing as well as statistical exercises were proposed and 26 laboratories submitted results. 相似文献
87.
考核模式改革是我校"教学重心转变"工作中教学模式改革的重点和核心内容之一。本文通过对《特殊痕迹检验》课程考核模式的改革与实践,提出了适应本专业学生教学练战一体化的考核模式。 相似文献
88.
心理学的研究离不开各种实验,心理测试技术(俗称"测谎"技术)实际上就是心理学实验方法的一种。此项技术在我国的研究应用已经30多年,辅助司法领域进行了大量的实案心理测试①。不仅对陷入侦破困境的案件起到很大作用,也对心理学在实战领域的应用研究提供了一种实验范式。对进行心理测试案件的研究发现,很多的变量都会影响到测试的结果,进而影响测试效度。被测人②的心理状态是其中很重要但是很容易被忽视的因素之一,这是一个非常值得关注研究的领域,不仅关系到心理测试技术的发展,也关系到司法领域人权和司法公正问题。 相似文献
89.
Elizabeth A. McGaw Ph.D. ; David W. Szymanski Ph.D. ; Ruth Waddell Smith Ph.D. 《Journal of forensic sciences》2009,54(5):1163-1170
Abstract: With improvements in manufacturing procedures, comparing physical characteristics of paper samples may not offer sufficient discrimination among different vendors. In this work, the potential to differentiate paper samples based on trace element concentrations was investigated. Paper samples from two different vendors were microwave-digested and trace element concentrations (Mg, Al, Mn, Fe, Sr, Y, Ba, Ce, and Nd) were determined using inductively coupled plasma–mass spectrometry. Differences in concentration were assessed statistically using two-way ANOVA and Tukey's honestly significant differences test. Elemental concentrations were shown to be consistent across a single sheet as well as within a single ream of paper for each vendor. Reams from vendor A were differentiated based on Al and Ba concentration while reams from vendor B were differentiated based on Mg, Mn, and Sr concentrations. Paper was differentiated according to vendor based on significant differences in Ba, Sr, Ce, and Nd concentrations. 相似文献
90.
Yoshinori Nishiwaki M.S. Seiya Watanabe M.S. Osamu Shimoda B.S. Yasuhiro Saito B.S. Toshio Nakanishi B.S. Yasuko Terada Ph.D. Toshio Ninomiya Ph.D. Izumi Nakai Ph.D. 《Journal of forensic sciences》2009,54(3):564-570
Abstract: High‐energy synchrotron radiation x‐ray fluorescence spectrometry (SR‐XRF) utilizing 116 keV x‐rays was used to characterize titanium dioxide pigments (rutile) and automotive white paint fragments for forensic examination. The technique allowed analysis of K lines of 9 trace elements in 18 titanium dioxide pigments (rutile), and 10 trace elements in finish coat layers of seven automotive white paint fragments. High‐field strength elements (HFSE) were found to strongly reflect the origin of the titanium dioxide (TiO2) pigments, and could be used as effective parameters for discrimination and classification of the pigments and paint fragments. A pairwise comparison of the finish coat layers of seven automotive white paint fragments was performed. The trace elements in the finish coat layers detected by the high‐energy SR‐XRF were especially effective for identification. By introducing the trace element information of primer and electrocoat layers, all the automotive white paint fragments could be discriminated by this technique. 相似文献