猪日本乙型脑炎病毒NS1基因重组腺病毒的构建及表达

应用RT-PCR方法扩增出猪日本乙型脑炎病毒(JEV)的NS1基因,通过SalⅠ EcoRⅠ双酶切后把该基因插入到经过同样双酶切的穿梭质粒pDC315中。重组穿梭载体经过双酶切和PCR鉴定后进行测序,序列测定正确。将获得的重组穿梭质粒与腺病毒骨架质粒共转染293细胞后获得重组腺病毒。PCR鉴定及间接ELISA检测的结果证明所构建的重组腺病毒成功地表达了JEV的NS1。     

论"看"在日语中的运用

文中从中文的“看”分析出看字在日文中表达时的微妙区别,并根据它们的词性和日语的习惯表达说明了简单的看字的区别。     

论日本女训中的儒家女性观

受中国女训影响,随着儒家思想的兴盛以及封建家制度的确立和完善,日本女训在近世得到了广泛的发展和普及。对男尊女卑等儒家女性观的吸收和借鉴是日本女训最为鲜明的特色之一,但这种借鉴并不是全盘照搬,而是根据日本的社会现状,有选择地加以模仿。探讨其中所体现的儒家女性观,有利于我们把握当时日本女性的生存状况及其对当今日本女性社会地位的影响。     

中日稻作起源研究回顾

1973年河姆渡遗址发现后,中日两国学者对稻作起源的研究有了突破性进展,对稻作起源年代的认定更为明确,尤其是两国学者对稻作起源区域的高度认同以及研究方法、内容上的互补,大大丰富了稻作文化的内涵。     

冷战结束后日本马克思主义研究综述

日本马克思主义在经受了苏东剧变所带来的世界反马克思主义浪潮的严峻考验后,逐渐复苏并呈现出蒸蒸日上的发展态势.本文对此进行了梳理,并对全球化理论、环境思想以及社会主义理论等日本马克思主义研究的理论热点进行了重点概述.     

THIS WEEK

正A WAlK FoR PEACE ANd REMEMBRANCEQueen Margrethe II of Denmark walks across Peace Square during her visit to the Memorial Hall for the victims of the Nanjing Massacre by Japanese Forces in Nanjing,capital of east China’s Jiangsu Province,on April 27.     

清末民初浙江监狱改良与日本

本文为中国近代转型时期以日本为媒介移植西方法律制度的个案事例研究。着重关注的是清末官绅对日考察,不同于已有较多成果的清末留日研究。据《申报》记载,包括浙江在内的十省于1906年联合派遣正佐官员五十名赴日考察监狱。该史料已为相关研究者所关注,但这些"调查日本监狱员"在日考察的实际状况和回国后的活动至今不为人知。在梳理浙江省派员名单,挖掘其在日活动史料基础上,进一步追踪其中三人回国后在浙江省监狱改良中作出的贡献。     

The artist as a medium and the artwork as metaphor in Murakami Haruki’s fiction

Abstract

In many of Murakami Haruki’s works, the protagonist is in a profession close to the world of literature, although usually not exactly a writer. In their role as translators, advertising copywriters, journalists, or editors, Murakami’s protagonists are invested with the task of rearranging fragments of reality into narrative or artistic form, and/or of transmitting a message. This process is emotionally charged, often involving a supernatural dimension. While the process of reconstructing a narrative is largely unconscious, the narrator’s choice to act in this capacity is deliberate, and is presented as a way of fulfilling his responsibility towards others. I read this element as an expression of Murakami’s vision of the role of a writer in society. The figure of the artist-narrator takes an interesting form in Kishidanchō-Goroshi (Killing Commendatore, 2017), where the protagonist, a visual artist specialising in realistic portraits, decides to pursue a more creative approach to painting that leads him to a series of supernatural adventures. Through a close reading of the novel, this article investigates how Murakami’s portrayal of the artist as a shaman-like figure functions like a metaphor for the role of the literary author as a catalyst that enables people to connect with their inner self and with each other.     

Mobilizing from the Margins: Domestic Citizen Politics and Yasukuni Shrine

Prime Minister Koizumi’s six consecutive annual visits to Yasukuni shrine played a key role in initiating a new phase of domestic citizen political mobilization not seen since the early 1970s. This paper is based on field research during the Koizumi years (2001–2006) centering on domestic groups that conduct activities in “protection” of or “opposition” to Yasukuni shrine. As a study of street-based politics, this paper seeks to uncover the processes, strategies, and outcomes of citizen responses to elite political action at Yasukuni Shrine as well as explore meaning of their actions within the context of Japan’s democratic polity.

论日本自民党派阀政治回归的必然性

日本自民党前任总裁小泉纯一郎在其当政期间采用了一系列标新立异的政治手法,使该党传统派阀政治一时间似乎面临消亡的境地。但是,小泉刚离任不久,曾经红极一时的"小泉政治"即迅速走向终结,派阀政治随之卷土重来。究其原因,小泉政治其实只是特定时期的特殊产物,而自民党的派阀则由于建立在日本社会、历史和文化基础之上,不可能轻易消亡。不过,对于自民党来说,不管是小泉政治还是派阀政治,从根本上说一切都是为了权力。