UPLC/Q-TOF-MS 和 PLS-DA 在鸦片生物碱检测及分类判别中的应用

目的建立了鸦片中吗啡、可待因、蒂巴因、罂粟碱、那可汀5种常量生物碱和牛心果碱、劳丹宁、劳丹素等12种痕量生物碱同时分离鉴定的超高效液相色谱-高分辨四极杆飞行时间串联质谱（UPLC/Q-TOF）方法。方法采用Agilent Eclipse Plus C18RRHD（2.1 mm×100 mm,1.8μm）色谱柱,以10 mM甲酸铵溶液和乙腈体系梯度洗脱,使用ESI离子源,正离子模式下采集数据。结果借助UPLC的快速分离和Q-TOF-MS测定的精确分子量和二级碎片信息,实现了17种生物碱的检测。应用SIMCA-P软件对78份国产鸦片和200份缅甸产鸦片进行偏最小二乘法判别分析（partial least squaresdiscriminate analysis,PLS-DA）,比较不同地区鸦片样品中生物碱的差异,建立了区分国产鸦片和缅甸产鸦片的判别方法。结论所建立的方法用于鸦片样品中常量和痕量生物碱的检测及鸦片样品的产地判别,具有快速、高效、灵敏、准确的特点。     

抑制消减杂交技术筛选大鼠急性心肌缺血后差异基因

目的采用抑制消减杂交技术,筛选早期心肌缺血关的RNA分子,为相火研究和应用提供依据。方法10只SD雄性大鼠结扎冠状动脉前降支1h,制作心肌缺血模型。以缺m心肌的RNA分了为实验组,以非缺血区Ⅸ的RNA分子为对照组,朋Chmtech公司抑制消减杂交试剂盒进行杂交。经蓝白斑筛选阳性克隆测序斤经过斑点杂交确认,构建早期大鼠心肌缺血后抑制消减杂交文库,所得序列和Genbank数据库比对。结果筛选到10个阳性克隆和11个未知基因的表达序列标签（expressedsequencetag,EST）,按照功能分类,包括代谢相关的酶类基侧,核糖体和翻译相关的基斟,心肌重塑相芙的基旧等,其叶1组织蛋白酶LI,basi面n等基因等是首次报道与早期心肌缺血相芙。结论采用抑制消减杂交技术获得的差别表达基因可为早期心肌缺血提供新的目标分子。     

24个基因座复合扩增系统的应用

目的建立24个基因座的复合扩增系统,并对其性能指标进行评价。方法选择24个基因座（包含D8S1179、D5S818、D2S1338、D18S51、D6S1043、D2S441、D3S1358、vWA、D19S433、D16S539、CSF1PO、Penta D、D22S1045、D13S317、D1S1656、D7S820、TPOX、Penta E、D10S1248、TH01、D12S391、D21S11、FGA等23个STR基因座及1个Amelogenin基因座）;合成引物,上游端标记荧光染料;收集DNA数据库建库血痕及口腔拭子样本及相关11种动物样本,采用本文方法进行复合扩增;并对方法的准确性、灵敏度、稳定性、种属特异性、检材适用性、混合样本的检验等系统性能指标进行验证。结果本文复合扩增系统对最长保存9年的各种血痕检材完整分型成功率为100%,且均衡性良好,无非特异性扩增,口腔拭子的成功率为97.8%,灵敏度达125pg,对含有抑制剂样本,在血红素浓度≤600μmol/L,腐殖酸浓度≤50ng/μL时检出效果稳定,种属特异性好。结论本文24个基因座复合扩增系统在DNA数据库建设中具有较好的应用价值。     

Recent Development in the South China Sea and Vietnam＇ s Approaches

This paper examines the new developments in the South China Sea （SCS） disputes between Vietnam and China and their causes. A substantial part investigates the approaches Hanoi has adopted to deal with China＇s potential aggression in the SCS. The first is the ＂multilateral＂ approach, which aims to make full use of Association of Southeast Asian Nations （ASEAN） and ASEAN-centric multilateral security dialogues as useful leverage for the creation of a regional Code of Conduct （COC）. The second approach involves the strengthening of ＂self-help＂ option by forging new developments in defense capabilities, especially naval power, to deter its northern giant from using force in the SCS. The third approach is the combination of two methods--pursuing engagement alongside a stronger defense and using what might therefore be called ＂defense diplomacy＂. These three approaches are not exclusive, but mutually reinforcing, and any shift in priority in each of the three options depends on China＇s revised policy in the SCS.     

Maastricht Treaty in the Shaping and Development of the Common Foreign and Security Policy （CFSP）

For the European Union （EU）, it discusses how to deliver （great project） European unification. Motivated by the idea that the association of European countries is a far better alternative to the previous mutually confronted Europe, a project in the following decades led to the emergence of a new and unprecedented work on European soil--EU. Its present form is the product of more than 50 years of evolution of European integration, which today despite initial six Western European countries including the former main rivals Germany and France include 27 member countries of the union, as well as many other countries with candidate status for membership in the EU. Seen in the development context, the union is not a preconceived model of the association of European countries, but is the product of a complex multiple decade-long process of integration in which different actors are involved. Within this process, depending on the achieved level of integration, the union received a different shape. In its present form and name as the EU, it occurs even in the early 90s of the last century, long before it passed through small-scale forms of integration.     

The Reforms in the Institutional Structure of the European Union in Compliance With the Lisbon Treaty

The necessary adjustment to the political architecture in Europe is considered to happen with the Treaty of Lisbon. The treaty is an attempt to make adjustments to the decision-making process, ensuring democratic legitimacy, strengthening of the institutions, and enabling political action worldwide. With the Lisbon Treaty, a change has been made in the institutional structure of the European Union （EU）. The Treaty of Lisbon brings together more than a half-century history of integration, which began with the decision for establishing the European Coal and Steel Community （ECSC） on April 18, 1951. The view on the whole, more than 50 years of integration, shows that the European unification process all the time is not out of the spirit of all issues related to the details of common goals and values, but goes in the direction of one political vision of： peace and prosperity, freedom and mobility, knowledge of democracy, populism and tolerant coexistence. The Treaty of Lisbon is in the way to improve the capabilities for action of the democracy in Europe.     

Thought and Practice of Cultivating Young Talent in China From 2002 to 2012

The goal of building a well-off society to benefit the majority of all people, and undertaking national construction and development, need more young talents to play intellectual role and show business skills. So Hu Jintao and Communist Party of China （CPC） put a large number of thoughts and practice of cultivating young talents. This paper consists of three parts： the thoughts of cultivating young talents, the practice of cultivating young talents, and the conclusion. The quantity and quality of training young talents have improved by leaps and bounds through these thoughts and practice. The young talents who get the attention of the world and contribute their due share to the development of the world have become a group. So the thought and practice of cultivating young talents are not only the wealth for China, but also the important wealth for the whole world.     

China＇s Political Stability and Comprehensive National Power： A Case Study of the Conflict in Xinjiang

The concept of comprehensive national power （CNP） is proposed and developed by several Chinese scholars and academic institutions. Many material capabilities, for example, economic growth and military might, are incorporated into the concept to measure China＇s national power vis-h-vis other major powers, especially the U.S. This paper, however, contends that understanding China＇s CNP through material capabilities is only part of the story. Yet, China＇s political stability is by no means assured and fully incorporated into the concept. China has undoubtedly faced many threats and challenges to its political stability. Apart from the Two Ts＇ problem （Taiwan and Tibet）, the conflict in Xinjiang not only threatens China＇s political stability since the establishment of the People＇s Republic of China （PRC） in 1949, but also affects its CNP as a whole. This paper concludes that measuring the genuine CNP should be based on the factors from which a country would earn or benefit, and also on those of which it would have to pay a price. Needless to say, the conflict and political instability in Xinjiang are an example of the price which China has to pay.     

关于中国—新加坡经济走廊建设的政治学思考

中国—新加坡经济走廊的建设不仅是一个经济问题,更是一个政治问题,它深受该区域国家内外政治因素影响,要推动该经济走廊建设,需要政治智慧和策略,文章提出推动中新经济走廊建设的策略建议。     

专家证人模式与司法鉴定模式之比较

专家证人模式与司法鉴定模式是英美法系和大陆法系在解决专门性事实问题上两种不同的模式。两者在法律文化基础、有关专家的诉讼地位和资格、程序启动、质证、证据能力和证明力上都有很大的不同。然而,由于两种模式都存在其固有的缺陷,近年来各国都在进行相应的改革,出现了相互融合和借鉴的趋势。