运用酚-氯仿法结合磁珠法提取蝇蛆体内人类DNA

目的建立运用酚-氯仿法结合磁珠法从蝇蛆嗉囊内提取人类DNA的方法,从而提高STR分型检验的灵敏度。方法采用酚-氯仿法对蝇蛆嗉囊内容物中的人类DNA进行提取,提取产物经磁珠法纯化浓缩后用QuantifilerTM人类DNA定量试剂盒在7500型实时荧光定量PCR仪上进行PCR定量,再用AmpF■STR IndentifilerTM试剂盒在3130XL-Avant遗传分析仪上对这些DNA样本进行STR分型。结果本研究建立的方法可增加模板DNA浓度约为单独使用酚-氯仿法的2倍。用此方法提取到的DNA浓度[（0.218±0.041）ng/μL]可获得全部16个STR分型结果。结论酚-氯仿法结合磁珠法可以有效地提高提取到的人类DNA样本STR分型检验的灵敏度,对于从事法医昆虫学方面研究的工作者有较好的实用价值。     

借鉴中井院特色教法 提升政工干部培训质量——《中国井冈山干部学院发展战略研究报告》研读启示

中国井冈山干部学院实施品牌战略,形成了独具特色的9种教学方法。提升政工干部培训质量,必须积极学习借鉴中井院教法。讲授式教学做到专题要专,教员精讲;研讨式教学做到准确设问,研究为先;现场式教学做到整体设计,教员主导;案例式教学实现自主开发案例,防止教学偏差。     

无问而问与不答之答——读邓正来《中国法学向何处去》

中国法学受着一种源自西方的"现代化范式"支配,只有充分、坚实地证明当下中国法学已经从根本上无力解释和解决中国法律实践中所存在的基本问题或关键问题,才能断言中国法学存在着总体性危机.邓正来先生并没有有效进行这种证明,无法断言中国法学存在着总体性危机,"中国法学向何处去"就是"无问而问",即便不是一个假问题,在它的提出理路里也很难被认为是一个真问题.邓正来先生并没有给出"中国法律理想图景"或"邓正来的中国法律理想图景",他并没有真正回答"中国法学向何处去"这一问题,但是在他的论述中给出了一个问题解决套路.因种种原因,该问题解决套路的有效性十分可疑.经过邓先生的立论和笔者的驳论,显示出一些对中国法学发展可能有意义的问题.     

Was It Worth It? The Territorial Reform in the Canton of Glarus

This paper examines the financial impact of the 2011 reform of the local governments and jurisdictions in the canton of Glarus. After a popular vote 70 public entities were reorganized to form three large uniformed communes. Previous research showed that amalgamations do not automatically lead to savings in public expenditures and economies of scale. We empirically apply the synthetic control method and show that the territorial reform realized some savings in public expenditure. However, the effect of the reorganization of tasks and responsibilities between the canton and the communes was more influential. The reform must be understood as a horizontal and vertical venture that encompasses both the communes and the canton, much more far‐reaching than a pure local territorial reform.     

法院解释条约的原理与中国机制的构建

条约是公认的国际法,许多国家在司法实践中都将条约作为法院裁判案件的适用依据。但在我国,宪法并未规定条约在其中的地位,条约主要是立法转化适用而不是吸纳适用,其也不是行政诉讼案件的适用和参照依据。因此,有必要构建法院解释条约的机制,明确法院解释条约发生的条件,同时建立参照条约的个案解释方法,法院在个案审理中可以直接以非人权条约为适用依据来裁判案件,援引人权条约或者参照条约进行论证和说明理由。     

Political theory in historical context. Reflections on Democratic Justice and the Social Contract by Albert Weale

In Democratic Justice and the Social Contract, Weale defends a contractarian theory of social justice following what he calls the ‘empirical method’, which consists in grounding ethics and politics on the observation of concrete examples of social contracts, rather than abstract speculations. In this paper, I will make three critical remarks. First, the empirical method is open to the same objections usually raised against more abstract approaches to social contract theory: by an appropriate choice of the starting point, one can justify any ethical or political position. Second, Weale’s focus on the societies that were successful in managing common pool resources appears arbitrary: other social organizations (e.g. hunters and gatherers societies) would be a more obvious choice. Finally, in following the empirical method, philosophers must be willing to import into ethics and politics the same problems of interpretation one encounters in theoretical social sciences. As an example, I will show that Weale’s position on the welfare state depends on the interpretation he gives of some practices observed in the societies he chooses as models. Different interpretations of the same practices would induce Weale to revise his positions.     

Where you lead we will follow: a longitudinal study of strategic political communication in election campaigning

The ways in which election campaigns are planned, organized, and conducted have changed considerably during recent decades, and political parties constantly employ new ideas and practices in order to communicate as strategically and effectively as possible. The concept of strategic political communication refers to an organization's purposeful management of information and communication to achieve certain political goals. In this article, we focus on the role of the individual political party in developing election campaigning. The empirical material covers the 2002, 2006, and 2010 Swedish national election campaigns and illustrates how political parties lead and follow each other in the development of strategic political communication. By exploring party agency, this article contributes to the understanding of how ideas and practices of strategic political communication emerge and develop over time in election campaigning.     

激光雕刻印章印文真伪鉴定

激光雕刻印章是当代科技集激光技术、计算机技术、数控技术、电子技术等为一体的刻印技术,已广泛应用于社会。激光雕刻印章印文真伪鉴定成为文检员面临的新课题。本文从激光雕刻印章的原理及程序、印章印文的特点、印章印文的检验方法、鉴定时可使用的特征、形成非本质差异的原因等方面进行了探讨与研究。     

赛洛新和赛洛西宾分析方法研究进展

致幻剂是一类低剂量就使人意识、知觉和视觉异常,引起幻觉和情绪障碍的精神活性物质.赛洛新和赛洛西宾是一类具有致幻作用的色胺类化合物,存在于裸盖伞属和斑褶菇属的致幻蘑菇中.当前滥用及娱乐性使用含此类物质的致幻蘑菇的现象日趋严重,由此引起的危害公共安全案(事)件不断增多.因此,对于致幻蘑菇滥用的鉴定是当前司法鉴定领域的重要需...     

秉承中国传统文化的现代平面艺术设计方法研究

中国传统文化博大精深,是艺术设计者取之不尽、用之不竭的创作源泉,我们应该如何对待传统文化与现代艺术设计的关系,如何有效地将传统文化元素与现代平面艺术设计创作完美结合,创造出有深刻中国文化内涵的平面设计作品,是一个值得艺术设计者思考并深入研究的课题.