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151.
MMP-2和MMP-9与脑损伤及其法医学意义 总被引:1,自引:1,他引:0
基质金属蛋白酶2和9(MMP-2、MMP-9)是基质金属蛋白酶(matrix metalloproteinases,MMPs)家族中的两个成员,可降解Ⅳ型胶原、层粘连蛋白等基质成分,从而使血脑屏障结构破坏,通透性增加,导致血管源性脑水肿,在脑血管疾病和脑损伤形成过程中起着重要的作用。在损伤后不同时段,MMP-2和MMPO含量及分布均呈现一定的波动性,探讨这些问题对脑损伤的治疗及在法医学中推断脑损伤时间有十分重要的意义。本文就这些问题的相关研究作一概述。 相似文献
152.
Robert S. McLaren Martin G. Ensenberger Bruce Budowle Dawn Rabbach Patricia M. Fulmer Cindy J. Sprecher Joseph Bessetti Terri M. Sundquist Douglas R. Storts 《Forensic Science International: Genetics Supplement Series》2008,2(4):257-273
Several laboratories have reported the occurrence of a split or n − 1 peak at the vWA locus in PowerPlex® 16 and PowerPlex® ES amplification products separated on 4- and 16-capillary electrophoresis instruments. The root cause of this artifact is post-PCR reannealing of the unlabeled, unincorporated vWA primer to the 3′-end of the tetramethylrhodamine (TMR)-labeled strand of the vWA amplicon. This reannealing occurs in the capillary post-electrokinetic injection. The split peak is eliminated by incorporation into the loading cocktail of a sacrificial hybridization sequence (SHS) oligonucleotide that is complementary to the vWA primer. The SHS preferentially anneals to the primer instead of the TMR-labeled strand of the vWA amplicon. In addition, the n − 10/n − 18 artifact that may be seen at the vWA locus was determined to be due to double-stranded amplicon formed post-electrokinetic injection into the capillary. This was also eliminated by adding in two Complementary Oligo Targets (COT1 and COT2) in addition to the SHS oligonucleotide into the loading cocktail. These three oligonucleotides are complementary to the 33 bases at the 5′-end of the unlabeled vWA amplicon strand and the 60 bases at its 3′-end and therefore compete for hybridization to the TMR-labeled amplicon strand. Incorporation of these three oligonucleotides in the Internal Lane Standard 600 (ILS600) eliminate both the split peak and n − 10/n − 18 artifact in PowerPlex® 16 and PowerPlex® ES amplification products without affecting sizing of alleles at the vWA locus or any locus in the PowerPlex® 16, PowerPlex® Y, PowerPlex® ES, AmpFlSTR® Profiler Plus® ID, AmpFlSTR® Cofiler®, and AmpFlSTR® SGM Plus® kits. 相似文献
153.
Jrg Michael Dostal 《The Political quarterly》2019,90(2):286-296
Germany's Christian Democrats have started preparing for the time after Angela Merkel. After ten years as German chancellor facing a weak opposition, Merkel unexpectedly split the country in late 2015 and early 2016 because of her ‘open border’ policies that allowed more than 1 million refugees and migrants to rapidly enter Germany. Her management of the subsequent crisis was largely considered a failure and her party suffered a series of dramatic election defeats. Reacting to the negative electoral feedback, and in particular the breakthrough of the rightist and anti‐immigration Alternative for Germany (AfD), the Christian Democratic Union organised an intra‐party contest to replace Merkel as party leader. Three candidates with different political profiles, Annegret Kramp‐Karrenbauer, Friedrich Merz and Jens Spahn, contested the election. By voting for Kramp‐Karrenbauer, the CDU membership voiced support for maintaining a large‐scale political coalition based on efforts to find compromises between different party wings and social and cultural interests. 相似文献
154.
ANDREAS DÜR 《European Journal of Political Research》2019,58(2):514-535
Through what mechanism do interest groups shape public opinion on concrete policies? In this article, three hypotheses are proposed that distinguish between the effect of the arguments conveyed by interest groups and the effect of interest groups as source cues. Two survey experiments on the proposed Transatlantic Trade and Investment Partnership (TIPP) and the 2015 Paris Agreement on climate change allow the testing of these hypotheses. The resulting evidence from several countries shows that, with respect to interest groups’ attempts at shaping public opinion, arguments matter more than their sources. This is so even when accounting for people's trust in the interest groups that serve as source cues and for people's level of information about a policy. The finding that interest groups affect public opinion via arguments rather than as source cues has implications for the literature on elite influence on public opinion and the normative evaluation of interest group activities. 相似文献
155.
Seismologists have reported that a majority of recent earthquakes in Oklahoma have been triggered by the activities of oil and gas companies. Despite this fact, there is evidence of strong opposition toward earthquake mitigation policy. In this article, we argue that how individuals define issues affect their policy choice. Furthermore, we incorporate the concept of venue shopping from the literature on macro theories of the policy process to investigate the effect of problem definition in shaping individual venue preference for policy choice. Using unique survey data, we find that problem definition, particularly issue causality and issue image, is strongly related to individual support for earthquake mitigation policy. However, a more nuanced relationship between individual problem definition and venue preference is observed. Our findings contribute to scholarly endeavors to understand the politics of problem definition at an individual level, which may be the precursor of understanding policy choices at the institutional level. 相似文献
156.
FIDL is a fast and automated DNA identification line which represents a series of software solutions automating the process from raw capillary electrophoresis data to reporting. This retrospective study provides insight in the numbers of cases, turnaround time, results compared to the standard workflow and the benefits automation has in a large volume workflow. 相似文献
157.
158.
目的探讨肌肉浸渍液电导率(electrical conductivity,EC)、pH两指标联用在PMI推断上的意义。方法 SD大鼠处死后,保存在25℃环境中,于不同PMI提取大鼠后肢肌肉制备匀浆液,测定浸渍液EC和pH。分析EC、pH两指标联合使用推断PMI的意义。结果 EC、pH两指标随PMI均呈现阶段性变化:1d内,pH明显下降,EC无明显变化;2~3d,EC、pH均快速增大;4~10d,EC继续增大,而pH变化不明显。EC、pH联合使用,有助于划分PMI时段,以及特定时段内指标的优化:1d内,选择pH最佳,2~3d,EC、pH联用最佳,4~10d,选择EC最佳。结论 EC、pH联用有助于提高PMI推断的准确性。 相似文献
159.
Soumaila Gansonré 《Development in Practice》2019,29(3):326-335
This article investigates off-farm work decisions by smallholder farm households. Using data on 1,101 households from Burkina Faso, it demonstrates that in addition to the usual household socio-demographic characteristics, remittances, access to information and beneficiary of specific training affect off-farm work decisions, as well as rainfall factors and idiosyncratic health shocks. Community characteristics such as accessibility and number of inhabitants are also key factors. While suggesting that off-farm work is less likely to be undertaken for accumulation purposes, these results have important policy implications. In particular, policy measures should consider each segment as a separate entity. 相似文献
160.
后金融危机时代,各国为从金融危机中尽快复苏经济普遍加强了投资促进工作,国际投资促进机构运行呈现新特征。通过深入研究新特征,密切关注其走势及采取的积极应对措施。为进一步理顺我国投资促进体制机制、创新投资促进方式、推动扩大内需、提升投资服务质量、提高投资促进效益提供启示,深化我国投资促进工作,提升国际竞争力。 相似文献