可控细胞膜孔形成蛋白基因的克隆与表达

根据Wood 4 6株金黄色葡萄球菌α溶血素 (α HL)基因序列设计了引物 ,用高保真PCR从 180 0株金黄色葡萄球菌基因组DNA中扩增出了 0 .9kb的α HL基因 ,序列测定结果显示 ,两菌株α HL基因的核苷酸序列有 6个碱基差异 ,但仅引起 1个氨基酸替换。用PCR分段克隆和定点突变技术将大肠埃希氏菌外膜蛋白A信号肽序列与α HL编码区融合 ,并将其第 130～ 134位氨基酸替换为 5个连续的组氨酸 ,获得的融合基因命名为H5基因。将其克隆入 pET 30a质粒 ,用获得的重组质粒pET H5转化感受态大肠埃希氏菌 ,获得分泌性表达H5的重组菌。经IPTG诱导后 ,重组菌表达出分子质量为 35ku的H5蛋白。经镍柱亲和层析纯化的H5对兔红细胞的半数溶血值为 180ng/mL ,能使鼠成纤维细胞PA317获得对台盼蓝和海藻糖的通透性 ,且能被Zn2 抑制。提示 ,表达的H5蛋白为可控细胞膜孔形成蛋白 ,能用作真核细胞内玻璃态保存的渗透剂     

onably estimate American options.Because Monte-Carlo simulation is a forward ind

is unable to correctly value American options.Binomial tree can be applied to pricing of all kinds of options,but it has a lot of flaws,such as accuracy.So when the number of step is very small,it affects the accuracy of the American options     

人DNA指纹检测的初步研究

根据随机探针检测DNA限制片段长度多态性的原理和人与鼠的髓鞘硷性蛋白(MBP)基因cDNA有90％以上同源序列的事实,我们选用rMBP-cDNA 3'端非表达区高度重复顺序的0.81kb片段作探针,检测用HaeⅢ酶解的人DNA限制性片段结果可以分解出22条谱带,受检的30例无血缘关系的个体之间,没有两个人的谱带是完全相同的,显示出此方法的高度特异性。本文还比较了若干DNA片段作探针和几种限制性内切酶检测人DNA指纹的结果。     

猪肝细胞微粒P_(450)体外代谢隐丹参酮的研究

用钙离子沉淀法制备猪肝细胞微粒体Ｐ４５０ 进行了体外代谢隐丹参酮的研究。用Ｌｏｗｒｙ 法测定肝微粒体蛋白含量平均为２ ．４６０ ±０ ．６９９ ｍｇ／ ｍ Ｌ;Ｏｍ ｕｒａ 法测定猪肝微粒体Ｐ４５０ 含量平均为０ ．１７６ ±０ ．０５３ ｎ ｍｏｌ／ ｍ ｇ 。从Ｐ４５０加还原型辅酶Ⅱ的体外代谢系统中分离纯化了２ 个主要代谢产物,经光谱分析鉴定为丹参酮ⅡＡ 与１羟基丹参酮ⅡＡ,并比较了隐丹参酮在猪体内外的代谢差异。     

vWFⅢ基因座扩增片段长度多态性及其法医学应用

研究短串联重复vWⅢ基因应扩增片段长度多态性及其法医学意义。应用STR－PCR分型法对200名中国辽宁汉族无关个体的短串联重复VWFⅢ的多态性进行调查。共检出8个等位基因,频率分布在0．003～0．333,基因长度范围为154～182bP,个人识别能力为0．921,非父排除率为0．581。群体调查证实该基因座符合Hardy－Weinberg平衡,家系调查结果表明其遗传符合孟德尔方式。该基因座可作为人类群体遗传学的一个有价值的多态性标记,可用于法医学个人识别及亲子鉴定。     

实验性脑挫伤GFAP,PCNA免疫组织化学研究

采用改进的Ｆｅｅｎｅｙ氏落体打击致Ｗｉｓｔａｒ大鼠脑挫伤模型,利用免疫组织化学染色,观察大鼠实验性脑挫伤后ＧＦＡＰ、ＰＣＮＡ的改变,用图像分析仪对免疫组化染色阳性细胞灰度和面积进行测量,ＳＡＳ统计软件分析,得出脑挫伤后ＧＦＡＰ、ＰＣＮＡ变化的时间规律性,为脑挫伤形成时间推断提供新的手段。ＧＦＡＰ阳性细胞面积和灰度伤后呈上升趋势,伤后３ｈ,即有显著增加（Ｐ＜０．０１）,至４天时,阳性细胞灰度,面积达到峰值（Ｐ＜０．０１）,伤后７天仍保持较高水平（Ｐ＜０．０１）。ＰＣＮＡ阳性细胞伤后１２ｈ出现,灰度呈上升趋势,面积呈下降趋势,伤后３天时达到最大值（Ｐ＜０．０１）。因此,ＧＦＡＰ、ＰＣＮＡ免疫组织化学染色阳性细胞的数量,灰度,面积改变有时间性规律;推断２～７天的脑挫伤则以ＧＦＡＰ、ＰＣＮＡ改变为主;脑挫伤后星形胶质细胞数目增多,只有很少一部分为星形胶质细胞增殖而来,且这种增殖在伤后３天时达到高峰     

Identification of a novel polymorphism in the X-chromosome region homologous to the DYS456 locus

During an extensive multipopulation study with Y-short tandem repeat (STR) loci, amplified using the AmpFlSTR Yfiler PCR amplification kit, amplification of a 71 bp fragment was observed in 2.32% of the male samples analyzed (N = 3141). By direct sequencing of this fragment, it was determined that the primer binding sequences were identical to those of the DYS456 locus. A T to G single-nucleotide polymorphism (SNP) enabled amplification of the 71 bp fragment. The SNP is located within an X-Y homologous region at Xq21.31 and was observed with the highest frequency within the African American and Sub-Saharan African populations in our study. Presence of SNP on the X chromosome did not interfere with the reliability of typing the DYS456 locus and the other Y-STR loci typeable using the AmpFlSTR Yfiler PCR amplification kit. Full profiles in a mixture of male:female at 1:4000 were obtained using the current configuration of the AmpFlSTR Yfiler kit even in the presence of female DNA containing the G variant.     

Genetic polymorphism for the PowerPlex 16 system from the Chinese Tujia Ethnic Minority Group

POPULATIONS: The population studied was the Tujia population, living in the Hunan Province, China. Their ancestors had lived in the region for at least three generations. As a Chinese minority group, the Tujia ethnic group, with a population of 8,028,133 (year of 2000), is mainly distributed in the Wuling mountain area in the three provinces of Hunan, Hubei, and Sichuan. The Tujia population have their own language, which belongs to the Tibetan-Myanmese Language Group of the Chinese-Tibetan Language Family, but the large majority have come to speak the Han and Miao languages, now that the Tujias have been largely assimilated.     

Regional psychiatric boards in Israel: expectations and realities

As laid down by Israeli legislation, the Regional Psychiatric Board constitutes one of the chief instruments in the monitoring of compulsory hospitalization, of compulsory outpatient treatment and of the quality of all inpatient care of the mentally ill. This article presents the findings of a study into the working of these boards. Taking a sample of every second application (N=168) over a 6-month period to one of Israel's six existing Regional Psychiatric Boards, an examination was made of the decisions issued by the board and of other aspects of its functioning. Explanations for the findings of this analysis are suggested, and generalizing from these findings, recommendations are offered for the improvement of the future functioning of all six Boards.