全文获取类型
收费全文 | 133篇 |
免费 | 9篇 |
专业分类
各国政治 | 3篇 |
工人农民 | 4篇 |
世界政治 | 3篇 |
外交国际关系 | 39篇 |
法律 | 48篇 |
中国共产党 | 1篇 |
中国政治 | 1篇 |
政治理论 | 33篇 |
综合类 | 10篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2021年 | 2篇 |
2020年 | 6篇 |
2019年 | 7篇 |
2018年 | 8篇 |
2017年 | 6篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 3篇 |
2013年 | 10篇 |
2012年 | 9篇 |
2011年 | 5篇 |
2010年 | 7篇 |
2009年 | 9篇 |
2008年 | 12篇 |
2007年 | 11篇 |
2006年 | 10篇 |
2005年 | 13篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1994年 | 1篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1984年 | 1篇 |
1978年 | 1篇 |
排序方式: 共有142条查询结果,搜索用时 15 毫秒
51.
H9N2禽流感病毒HA2基因重组杆状病毒的表达 总被引:1,自引:0,他引:1
从pMD18-T-HA阳性质粒扩增了H9N2亚型禽流感病毒的HA2基因,将扩增到的 HA2基因克隆至昆虫杆状病毒转移载体pBlueBacHis2A中。将其与杆状病毒共转染于Sf9昆虫细胞,经蚀斑筛选纯化重组杆状病毒,用其感染Sf9昆虫细胞,并优化表达条件。SDS-PAGE和 Western-blotting分析表明,表达产物的分子质量约为27 ku。Dot-ELISA分析表明,表达的HA2 融合蛋白可与鸡抗H9N2亚型血清发生特异性反应,而与H5和H7亚型抗血清间无交叉反应。 相似文献
52.
《Critical Horizons》2013,14(2):181-199
AbstractThe core argument of the paper is that the modern philosophical notion of self-constitution is directed against the prospect of human beings dissolving into idleness. Arguments for self-constitution are marked by non-philosophical presuppositions about the value of usefulness. Those arguments also assume a particular conception of superior experience as conscious integration of a person’s actions within an identifiable set of chosen commitments. Exploring particular arguments by Hegel, Kant, Korsgaard and Frankfurt the paper claims that those arguments are problematic in the various ways in which they suppose usefulness and explicitly or implicitly take extra-philosophical views of idleness. 相似文献
53.
54.
NICOLE RAFTER 《犯罪学》2004,42(3):735-772
Biological explanations shaped criminology at its inception, and today they are reemerging with fresh vigor and increased potential. But many criminologists do not understand how biological theories developed, what they contributed to criminology generally and where they went astray. This paper focuses on the work of Earnest A. Hooton, whose criminological studies, published in 1939, met with decidedly mixed reviews but were nonetheless discussed for decades in criminological textbooks. Information about a now half‐forgotten and misunderstood figure like Hooton, in addition to being useful in and of itself, contributes to the history of criminology as a discipline—a project essential to the field's ultimate maturity. It helps build a history of criminological knowledge. 相似文献
55.
从有肺炎症状的病猪中分离到1株H9N2亚型猪流感病毒(SIV)A/Swine/Shndong/1/02,对其进行了全病毒基因序列分析.结果表明,该毒株8个基因片段的核苷酸序列均来自禽流感病毒(AIV),与我国目前家禽中流行的H9N2亚型AIV毒株具有很高的同源性,与A/Duck/Hong Kong/Y280/97(H9N2)的同源性为94.1%~98.9%,与A/Chicken/Beijing/1/94(H9N2)的同源性为94.5%~98.2%;推导的其血凝素(HA)裂解位点处的氨基酸序列为P-A-R-S-S-R,完全符合H9亚型AIV欧亚分支中的类A/Chicken/Beijing/1/94亚分支的特征;基因分析结果表明,该分离株的所有基因片段均来源于H9N2亚型AIV,它可直接感染猪,并导致发病,但它并未在猪体内发生重组. 相似文献
56.
禽流感病毒A/Turkey/Canada/63毒株HA和NA基因的克隆及序列分析 总被引:8,自引:0,他引:8
用SPF鸡胚增殖禽流感病毒(AIV)A/Turkey/Canada/63毒株,提取病毒基因组总RNA,应用RT-PCR技术分别扩增该病毒分离株的HA和NA基因全片段,并克隆到pMD18-T载体上。测序结果表明,该毒株的HA基因全长为1745bp,含有完整的阅读框架,编码566个氨基酸;NA基因全长1467bp,编码469个氨基酸。BLAST序列比较结果显示,该毒株HA基因属于H6亚型,NA基因属于N2亚型。将获得的HA和NA基因与AIV数据库中H6和N2基因进行遗传演化分析,表明该毒株HA基因与其他H6基因核苷酸的同源性为80.5%~87.3%,氨基酸的同源性为88.0%~93.1%;NA基因与其他N2基因核苷酸的同源性为86.0%~93.5%,氨基酸的同源性为90.6%~96.3%。 相似文献
57.
58.
59.
Yesim Dogan Alakoc Omer Gokcumen Aysim Tug Timur Gultekin Erksin Gulec Theodore G. Schurr 《Forensic Science International: Genetics Supplement Series》2010,4(5):e135-e137
Due to the longstanding human presence in the region and the influence of social traditions, the genetic make-up of populations currently inhabiting Turkey (Anatolia) is quite complex. To characterize the patterns of genetic diversity in rural Central Anatolian villages, we analyzed samples collected at four local settlements for variation at 17 Y-chromosome STR and 15 autosomal STR loci. The resulting data reveal considerable diversity within these settlements, as well as some structure in the paternal genetic variation, with a limited number of haplotypes being shared between the communities. These findings have important implications for forensic studies of Turkish populations. 相似文献
60.