心脏肌钙蛋白T、I与心肌损伤及其法医学应用前景

心脏肌钙蛋白T、I具有很高的心脏特异性,敏感性高,能检测多种不同的心肌损伤,区别心肌损伤和骨骼肌损伤,并能用作心肌损害的危险程度分级和判断预后的指标,在临床上得到了广泛的应用。在法医学上也有一定的应用前景。     

走新型工业化道路：科技进步与产业结构优化

走新型工业化道路,应在保证传统产业的支撑和基础地位的条件下,优先发展高新技术产业,全面发展第三产业。     

Sequences of the Cytochrome C Oxidase Subunit I (COI) Gene are Suitable for Species Identification of Korean Calliphorinae Flies of Forensic Importance (Diptera: Calliphoridae)

Abstract:  Calliphorinae fly species are important indicators of the postmortem interval especially during early spring and late fall in Korea. Although nucleotide sequences of various Calliphorinae fly species are available, there has been no research on the cytochrome c oxidase subunit I (COI) nucleotide sequences of Korean Calliphorinae flies. Here, we report the full-length sequences of the COI gene of four Calliphorinae fly species collected in Korea (five individuals of Calliphora vicina , five Calliphora lata , four Triceratopyga calliphoroides and three Aldrichina grahami ). Each COI gene was amplified by polymerase chain reaction and directly sequenced and the resulting nucleotide sequences were aligned and analyzed by MEGA4 software. The results indicate that COI nucleotide sequences can be used to distinguish between these four species. Our phylogenetic result coincides with recent taxonomic views on the subfamily Calliphorinae in that the genera Aldrichina and Triceratopyga are nested within the genus Calliphora .     

Automated processing of forensic casework samples using robotic workstations equipped with nondisposable tips: contamination prevention

An automated process has been developed for the analysis of forensic casework samples using TECAN Genesis RSP 150/8 or Freedom EVO liquid handling workstations equipped exclusively with nondisposable tips. Robot tip cleaning routines have been incorporated strategically within the DNA extraction process as well as at the end of each session. Alternative options were examined for cleaning the tips and different strategies were employed to verify cross-contamination. A 2% sodium hypochlorite wash (1/5th dilution of the 10.8% commercial bleach stock) proved to be the best overall approach for preventing cross-contamination of samples processed using our automated protocol. The bleach wash steps do not adversely impact the short tandem repeat (STR) profiles developed from DNA extracted robotically and allow for major cost savings through the implementation of fixed tips. We have demonstrated that robotic workstations equipped with fixed pipette tips can be used with confidence with properly designed tip washing routines to process casework samples using an adapted magnetic bead extraction protocol.     

Morbidity Involving the Hallucinogenic Designer Amines MDA and 2C‐I*

Abstract: A case is presented of a 39‐year‐old woman who suffered severe debilitation because of a hemorrhagic stroke in the context of substance abuse. The patient presented to the emergency room with rapidly diminishing mental status, hypertension, and vasoconstriction; her friends provided a history of ingestion of cocaine, 3,4‐methylenedioxymethamphetamine (MDMA), and 2C‐I, a novel designer amine. A multi‐targeted LC‐MS/MS method for sympathomimetic amines and related drugs in urine detected and quantified 2C‐I and MDA, while ruling out MDMA. The cause of the stroke was determined to be an underlying cerebrovascular abnormality called Moyamoya, secondary to substance abuse. In clinical laboratories, gas chromatography–mass spectrometry or liquid chromatography–tandem mass spectrometry (LC‐MS/MS) confirmation of a positive amphetamine immunoassay is usually directed only towards amphetamine, methamphetamine, MDMA and MDA. This report demonstrates the utility of testing for a wider menu of compounds using LC‐MS/MS in order to better characterize the prevalence and toxicities of novel amines such as 2C‐I.     

Gesetzliches Vorzugspfandrecht der Eigentümergemeinschaft nach § 27 WEG 2002 – detaillierte Forderungsanmeldung für die Meistbotsverteilung im Exekutionsverfahren

Wenn Mindest-/Miteigentumsanteile eines s?umigen Wohnungseigentümers zwangsversteigert worden sind, müssen die entsprechenden gesetzlichen Vorzugspfandrechte nach § 27 WEG 2002 sp?testens in der Verteilungstagsatzung gem § 210 EO angemeldet werden, da diese von Amts wegen nicht zu berücksichtigen sind. Die Anmeldung hat alle anspruchsbegründenden Tatsachen, n?mlich Zeitpunkt der "rückst?ndigen", f?lligen Forderungen, H?he der angemeldeten Forderungen samt Rechtsgründen sowie Bestehen der gesetzlichen Vorzugspfandrechte und deren Deckung in den Meistboten, detailliert zu enthalten. Deshalb ist eine blo? "globale" Forderungsanmeldung unzul?ssig; sie muss vielmehr so aufgeschlüsselt werden, dass die Forderungen den einzelnen WE-Objekten und den die entsprechenden Mindestanteile sichernden gesetzlichen Vorzugspfandrechten zugeordnet werden k?nnen. Sollten einzelne in Exekution gezogene Mindestanteile samt den damit verbundenen WE-Objekten keinen Erwerber finden, dürfen die durch die gesetzlichen Vorzugspfandrechte gesicherten Forderungen nicht durch jene Meistbote befriedigt werden, die für die anderen, erfolgreich zwangsversteigerten WE-Objekte erzielt worden sind.     

Teacher Skill Development: Evidence from Performance Ratings by Principals

We examine the dynamic nature of teacher skill development using panel data on principals’ subjective performance ratings of teachers. Past research on teacher productivity improvement has focused primarily on one important but narrow measure of performance: teachers’ value-added to student achievement on standardized tests. Unlike value-added, subjective performance ratings provide detailed information about specific skill dimensions and are available for teachers in non-tested grades and subjects. Using a within-teacher returns-to-experience framework, we find, on average, large and rapid improvements in teachers’ instructional practices throughout their first 10 years on the job as well as substantial differences in improvement rates across individual teachers. We also document that subjective performance ratings contain important information about teacher effectiveness. In the district we study, principals appear to differentiate teacher performance throughout the full distribution instead of just in the tails. Furthermore, prior performance ratings and gains in these ratings provide additional information about teachers’ ability to improve test scores that is not captured by prior value-added scores. Taken together, our study provides new insights on teacher performance improvement and variation in teacher development across instructional skills and individual teachers.     

How Local Economic Conditions Affect School Finances,Teacher Quality,and Student Achievement: Evidence from the Texas Shale Boom

Whether improved local economic conditions lead to better student outcomes is theoretically ambiguous and will depend on how schools use additional revenues and how students and teachers respond to rising private sector wages. The Texas boom in shale oil and gas drilling, with its large and localized effects on wages and the tax base, provides a unique opportunity to address this question that spans the areas of education, labor markets, and public finance. An empirical approach using variation in shale geology across school districts shows that the boom reduced test scores and student attendance, despite tripling the local tax base and creating a revenue windfall. Schools spent additional revenue on capital projects and debt service, but not on teachers. As the gap between teacher wages and private sector wages grew, so did teacher turnover and the percentage of inexperienced teachers, which helps explain the decline in student achievement. Changes in student composition did not account for the achievement decline but instead helped to moderate it. The findings illustrate the potential value of using revenue growth to retain teachers in times of rising private sector wages.     

Molecular identification of the potentially forensically relevant cluster flies Pollenia rudis (Fabricius) and Pollenia vagabunda (Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

Cluster flies are represented by the genus Pollenia Robineau-Desvoidy, 1830 of the family Polleniidae Brauer and Bergenstamm, 1889. Their larvae are known to be internal parasites or predators of earthworms. Herein, we report for the first time the occurrence of the cluster flies Pollenia rudis Fabricius, 1794 and Pollenia vagabunda (Meigen, 1826) (Diptera: Polleniidae) on carcasses in Algeria and identify them through DNA barcoding. A region of the mitochondrial cytochrome c oxidase I gene (COI) was amplified and sequenced. Genetic distances were determined. A phylogenetic tree was constructed with the maximum parsimony method using 10 000 bootstrap replicates. A total number of 157 adults of P. rudis were collected together with 325 adults of Pollenia vagabunda. The occurrence of Pollenia on animal carcasses does not seem to be correlated with a particular stage of decomposition. All the sequences were correctly identified using the BLASTn tool from the GenBank database and the BOLD identification engine. Intra- and interspecific sequence divergence values were less than 1% and greater than 3%, respectively. COI barcodes obtained from this study were robust enough to identify and distinguish unambiguously between P. rudis and P. vagabunda. In the tree-based analysis, the cluster flies were all assigned to their respective species separately from each other confirming the morphological identification. These results provide DNA barcodes that contribute to the growth of reference databases and allow fast and accurate identification.     

From parish register to the “historical table”: The Prussian population statistics in the 17th and 18th centuries

This overview deals with the development of population statistics in Germany and Prussia, where different streams and trends in the field of statistics can be observed until the end of the 18th century: (1) the so-called university statistics (Staatenkunde); (2) political arithmetic; (3) table statistics; (4) “German Kameralia;” and (5) Prussian financial science. The most important sources for historical demographic research are the parish registers. In Germany, they start in the early or high Middle Ages as baptismal lists. In Prussia in the 16th century, parish registers consisted of entries and items of baptisms (births), marriages, and funerals (deaths). Based upon these parish registers at the end of 17th century in the Brandenburg Electorate, population lists were set up as registers or tables of population movement, as “general registers of the born, the married, the deceased, and the communicants.” Physicians and such Prussian medical authorities as the Collegium medicum and the Collegium sanitatis collected data about the causes of death and the longevity of human life (people more than 90 years old). In the course of 120 years during the 17th and 18th centuries, about 350,000 immigrants came to Brandenburg-Prussia. The Prussians developed excellent migration and census statistics in the form of historical tables. From the founding of the Prussian Office of Statistics in 1805 until its reorganization in 1809–1810, historical tables were set up using population statistics.