Allele frequencies of 20 Y-chromosomal short tandem repeat loci in a tribal population of Deccan Plateau, India

Population: Eighty male individuals from a nomadic tribal population belonging to Dravidian and Indo-Caucasian ethnicities from Deccan Plateau, Andhra Pradesh, India, were analyzed in the present study.     

Y-chromosomal STR haplotypes in two endogamous tribal populations of Karnataka, India

POPULATION: Approximately 5.0 mL of blood sample was collected from a total of 150 men belonging to two tribal populations of coastal Uttar Kannada district of Karnataka, with their informed written consent. Both the populations are endogamous and they belong to the Dravidian linguistic family. Halakki is a tribal group having a population size of c. 3383. They claim that they originally belong to Gujarat and Rajasthan, and migrated through Andhra Pradesh to Karnataka. Kunabhi is also a tribal population, c. 35,214 in number. They were hunters and gatherers but presently they practice agriculture.     

Concordance study between the AmpFlSTR MiniFiler PCR amplification kit and conventional STR typing kits

The AmpFlSTR MiniFiler polymerase chain reaction amplification kit developed by Applied Biosystems enables size reduction on eight of the larger STR loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded DNA samples. The MiniFiler Kit amplifies CSF1PO, FGA, D2S1338, D7S820, D13S317, D16S539, D18S51, and D21S11 as well as the sex-typing locus amelogenin. A total of 1308 samples were evaluated with both the MiniFiler and Identifiler STR kits: 449 African American, 445 Caucasian, 207 Hispanic, and 207 Asian individuals. Full concordance between Identifiler and MiniFiler Kits was observed in 99.7% (10,437 out of 10,464) STR allele calls compared. The 27 differences seen are listed in Table 1 and encompass the loci D13S317 (n = 14) and D16S539 (n = 10) as well as D18S51 (n = 1), D7S820 (n = 1), and CSF1PO (n = 1). Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed by reamplification of the samples and further testing using the PowerPlex 16 kit in many cases. DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift.     

Developmental validation of reduced-size STR Miniplex primer sets

This paper describes a developmental validation study of three Miniplex sets covering 12 of the 13 CODIS loci. As these new sets will be used for the analysis of degraded and low level DNA, the validation studies were performed using 100-125 pg of DNA, the lowest input level at which peak balance, peak intensity, and allele consistency were stable. To demonstrate the applicability of the Miniplex sets to forensic casework, these validation studies were completed in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM). A range of tests were performed including studies of concordance with standard multiplex kits, sensitivity and reproducibility, and PCR amplification conditions. Additionally, studies of mixtures, nonhuman and environmentally degraded DNA, and simulated forensic samples were performed. Our results demonstrate that Miniplex STR amplification procedures are a robust and sensitive tool for the analysis of degraded DNA.     

论成年在校大学生对父母的抚养费请求权——兼论《婚姻法司法解释(一)》第20条的缺陷与完善

在司法实践中,对于在校成年大学生起诉父母索要抚养费的相似案件,不同法院的判决往往截然不同。主要原因在于《婚姻法司法解释(一)》第20条对"不能独立生活的子女"的规定存在缺陷。在《婚姻法》相关内容细化之前,有必要通过司法解释作出明确规定,一是明确"父母有给付能力"为成年子女请求父母给付抚养费的前提;二是细化对"不能独立生活的子女"的解释,甄别情况肯定或否定在校成年大学生对父母的抚养费请求权。     

Using Frons Width to Differentiate Blow Fly Species (Diptera: Calliphoridae) Phormia regina (Meigen) and Protophormia terraenovae (Robineau‐Desvoidy)

Protophormia terraenovae (Robineau‐Desvoidy) (Diptera: Calliphoridae) and Phormia regina (Meigen) (Diptera: Calliphoridae) are morphologically similar blow fly species commonly used for estimating postmortem intervals. Field collection and storage of adults can result in color changes, in particular on calypters and palps; often collected specimens show damage such as wing fray or fungal growth. We measured the frons width: total head width ratio using photographs (ImageJ version 1.49) to differentiate these two species. Both sexes were distinguishable to species, with the greatest difference between males: 12.34% P. terraenovae versus 1.62% P. regina, less so for females: 40.25% P. terraenovae, versus 33.65% P. regina. Incorporating this feature into future blow fly keys would help with distinguishing field‐caught specimens when other features are obstructed.     

新形势 新判断 新战略——解读党的十八大报告外交战略部分

在党的十八大上,在题为"继续促进人类和平与发展的崇高事业"的外交部分,党中央对当前的国际形势和中国外交策略进行了精辟有力的论述,准确分析了中国所处的国际形势,精确判断出中国的身份和责任,制定了中国新时期的外交战略。在报告中,中国共产党还根据国际国内形势的变化,提出了多个新亮点、新观念和新思路。     

Verteilungszeitraum

Es obliegt dem billigen Ermessen des Gerichts, den Beginn oder die Dauer des Verteilungszeitraumes festzusetzen. Zwingend muss der Verteilungszeitraum aber an den Verrechnungszeitraum anschlie?en. Jene Betr?ge, die nicht als Mietzinsschuldigkeiten – weil ohne gesetzliche oder vertragliche Grundlage – für die betreffenden Zeitr?ume geleistet wurden, sind aus der Hauptmietzinsabrechnung zu entfernen.