大肠埃希氏菌F18菌毛结构蛋白与黏附特性的关系

利用已表达的F18ab和F18ac菌毛各结构亚单位的融合蛋白(GST-FedA/ab、GST-Fe-dA/ac、GST-FedE、GST-FedF/ab、GST-FedF/ac)分组肌肉注射健康家兔,制备抗FedA/ab、Fe-dA/ac、FedE、FedF/ab、FedF/ac多价血清。结果表明,所制备的5种抗Fed多价血清均能凝集F18ab 大肠埃希氏菌107/86株和F18ac 大肠埃希氏菌8813株。利用抗大肠埃希氏菌F18菌毛主要结构亚单位FedA特异抗体和次要结构亚单位FedE、FedF特异抗体,研究了F18菌毛与小肠上皮细胞的黏附特性。结果发现,FedA抗体和FedE抗体单独和合并均不能抑制F18 菌与小肠上皮细胞的黏附,而单用抗FedF抗体即能明显抑制F18 大肠埃希氏菌与小肠上皮细胞的黏附,表明FedA和FedE与F18菌毛的黏附不具相关性,而FedF才是F18菌毛的黏附性结构亚单位。     

Population data on the forensic genetic markers: phosphoglucomutase-1, esterase D, erythrocyte acid phosphatase and glyoxylase I

Blood specimens from white and black sample populations from Baltimore, Maryland, were analyzed for the four most forensically important, polymorphic red cell enzyme systems-phosphoglucomutase-1, esterase D, erythrocyte acid phosphatase and glyoxalase I. The distributions of the phenotypes for each marker in each racial group were in Hardy-Weinberg equilibrium. The population data were similar to previously reported data for Whites and Blacks from different geographical locations within the United States.     

Reconsidering the southern Europe model: Dowry, women's work and marriage patterns in pre-industrial urban Italy (Turin, second half of the 18th century)

This paper aims to investigate and analyse the impact of the dowry and the endowment system on marriage and household patterns and on the labour market in 18th century Turin. At the same time it enquires into the reliability of the northern/southern Europe pattern for the study of this topic. Two points are developed. Firstly the paper shows that the dowry system coexists with a relatively high age at marriage because of the specific role played by the dowry in Turin society. Indeed, this was not a once-and-for-all established asset but was a negotiable and flexible resource that could be manipulated by the different actors concerned in the endowment. Moreover, the payment did not always take place immediately upon marriage, nor was the money quickly available. At the same time the paper examines the economic role of dowry in Ancien Régime households: by means of a juridical procedure couples could alienate this property, and use or invest it to ensure their well-being and/or their economic situation. Finally, the second part investigates the relationship between the dowry system and the participation of young girls and married women in the local labour market: their significant and crucial presence demonstrates that the dowry and its expectation was not a sufficient incentive to keep them out of the labour market.     

Detection of genotype recycling fraud in U.S. immigrants

Abstract: Relationship testing laboratories provide genetic evidence to support or refute claims of kinship between U.S. citizen petitioners and potential immigrant beneficiaries. One female beneficiary presented a male amelogenin type and alleles at 15 autosomal loci that were identical to an alleged brother’s. Laboratory records showed that her alleged father had petitioned to have 15 children emigrate from Ghana. The petitioner’s 15 paternity indices exceeded 10⁵, but the children shared only four short tandem repeat (STR) profiles, suggesting fraudulent reuse of genotypes in this alleged pedigree (AP). To determine the extent of this “genotype recycling,” I examined the laboratory’s 555 APs from Ghana and 532 control APs from Nigeria. Seventeen Ghanaian APs (3.1%) but no Nigerian APs showed genotype recycling. Of 90 tested people in the 17 APs, 56 shared identical STR profiles with others in their AP. Of these 56 people, 10 were petitioners with unexpectedly high parentage indices. Seven of 56 had amelogenin types that disagreed with their declared genders. Database searches for identical multilocus genotypes in allegedly different people would best detect this fraud.     

A new strategy for the discrimination of mitochondrial DNA haplogroups in Han population

Mitochondrial DNA (mtDNA) haplogroup discrimination is interesting not only for phylogenetic and clinical but also for forensic studies. We discriminated the mtDNA haplogroups of 570 healthy unrelated Han people from Zhejiang Province, Southeast China, by comprehensive analysis mutations of the hypervariable segments-I sequence and diagnostic polymorphisms in mtDNA coding region using real-time polymerase chain reaction (RT-PCR), which was compared with the widely used PCR and restriction fragment length polymorphism (PCR-RFLP) method. The results showed that in superhaplogroup M, haplogroup D was the most common haplotype within this assay to 24.6%, and in the other superhaplotype N, haplogroup B and F were the most common groups. Samples re-identified by PCR-RFLP showed the consistent results that were got with RT-PCR. In conclusion, the RT-PCR strategy appears to be an accurate, reproducible, and sensitive technique for the discrimination of mtDNA haplogroups, especially for mass screenings quickly and economically.     

From parish register to the “historical table”: The Prussian population statistics in the 17th and 18th centuries

This overview deals with the development of population statistics in Germany and Prussia, where different streams and trends in the field of statistics can be observed until the end of the 18th century: (1) the so-called university statistics (Staatenkunde); (2) political arithmetic; (3) table statistics; (4) “German Kameralia;” and (5) Prussian financial science. The most important sources for historical demographic research are the parish registers. In Germany, they start in the early or high Middle Ages as baptismal lists. In Prussia in the 16th century, parish registers consisted of entries and items of baptisms (births), marriages, and funerals (deaths). Based upon these parish registers at the end of 17th century in the Brandenburg Electorate, population lists were set up as registers or tables of population movement, as “general registers of the born, the married, the deceased, and the communicants.” Physicians and such Prussian medical authorities as the Collegium medicum and the Collegium sanitatis collected data about the causes of death and the longevity of human life (people more than 90 years old). In the course of 120 years during the 17th and 18th centuries, about 350,000 immigrants came to Brandenburg-Prussia. The Prussians developed excellent migration and census statistics in the form of historical tables. From the founding of the Prussian Office of Statistics in 1805 until its reorganization in 1809–1810, historical tables were set up using population statistics.     

生态文明：建设中国特色社会主义的道路——对十八大大力推进生态文明建设的战略思考

我们生活的时代是生态文明时代。生态文明是人类新文明。十八大提出“大力推进生态文明建设”战略．生态文明建设深刻融入和全面贯穿经济建设、政治建设、文化建设和社会建设“五位一体”的总体战略。中国共产党实施这一伟大战略,领导中国人民建设生态文明,这是中国道路,是建设中国特色社会主义之路,是中华民族伟大复兴之路。中国人民在党的领导下,在世界上率先走向建设生态文明的道路,将是中华民族对人类的又一个伟大贡献。     

Genetic profile of nine autosomal STR loci among Halakki and Kunabhi populations of Karnataka, India

POPULATION: Blood samples were collected from a total of 84 healthy and unrelated Halakki (44) and Kunabhi (40) populations, with their informed written consent. The geographic location of the sampled area is shown in Fig. 1. Both the populations are endogamous, and they belong to Dravidian linguistic family. Halakki is a tribal group having a population size of approximately 3383. They claim that they originally belong to Gujarat and Rajasthan, and migrated through Andhra Pradesh to Karnataka. Kunabhi is also a tribal population, who are approximately 35,214 in number. The male Kunabhi can be identified by their tattoo marks. A necklace is the symbol of married women. They were hunters and gatherers, but at present they practice agriculture.     

Population data for four population groups from the United States for the eleven Y-chromosome STR loci recommended by SWGDAM

POPULATIONS: Caucasian ( n =1243), African American ( n =1605), Hispanic ( n =454), and Native American ( n =104).