发挥统一战线工作优势为解决三农问题服务

在全面建设小康社会的目标下,农村的发展和"三农问题"的解决,最终仍需依靠农民的自身力量.改革开放以来涌现的农村打工者队伍,是农村社会的进步群体,是农村先进生产力的代表.统战部门可以通过加强工商联及其行业协会的建设,联系现有的工商业人士,引导农民工回乡创业,并在市场、项目、资金等方面相互合作,形成互动,打造区域经济,推动农村"多业化"的进程.     

霍布斯和洛克自然状态思想比较研究

自然状态是一种前公民状态,是社会契约论说明政府权力合法性来源的逻辑起点。尽管霍布斯和洛克同为17世纪英国政治思想家,但他们的观点却有很大的不同。他们从不同的人性出发,所描写的自然状态及自然状态中要遵循的自然法、享有的自然权利及走出自然状态的方式也有很大的差异。     

加味半夏白术天麻汤对椎基底动脉供血不足眩晕大鼠血管活性物质的影响

目的 观察加味半夏白术天麻汤对椎基底动脉供血不足（vertebrobasilar insufficiency,VBI）眩晕大鼠血管活性物质的影响。方法 将SPF级SD大鼠随机分为正常组、模型组、阳性对照组（用盐酸氟桂利嗪1.53 mg/kg灌胃治疗）、实验组（用加味半夏白术天麻汤141.7 mg/kg灌胃治疗）,每组10只;将切除的一侧横突间骨翼置入骨膜下椎动脉内侧,以复制VBI眩晕模型;连续给药14 d,采用酶联免疫吸附试验测定各组大鼠血浆内皮素-1(endothelin-1, ET-1)、血栓素B2(thromboxane B2, TXB2)、6-酮-前列腺素F1α(6-keto-prostaglandin F1 alpha, 6-Keto-PGF1α)、环磷酸腺苷（cyclin adenosine monophosphate, cAMP）的含量。结果 与正常组比较,模型组大鼠血浆ET-1、TXB2、6-Keto-PGF1α、cAMP含量以及TXB2/6-Keto-PGF1α比值均显著升高（P<0.01）;与模型组比较,阳性对照组和实验组上述指标均显著降低（P<0.05,或P<0.01）。结论 加味半夏白术天麻汤对VBI眩晕的治疗作用与降低血浆ET-1和cAMP含量,恢复TXB2/6-Keto-PGF1α比值有关。     

A pivotal role? The AFL–CIO and the 2008 presidential election

This article explores the role that organized labor played in the landmark presidential election of 2008. In particular, it explores the work of the American Federation of Labor–Congress of Industrial Organizations (AFL–CIO), which ran its biggest ever election campaign in 2008, spending upwards of $250 million. While there is a vibrant emerging literature on the election, particularly from political scientists and former reporters, labor’s role in the story has been largely overlooked. Drawing on new parts of the AFL–CIO’s papers, as well as interviews with key staffers and federation leaders, this article highlights the important – and overlooked – role that labor played in putting Barack Obama into the White House. Especially important were its extensive efforts to educate – and pressure – white members, many of whom had backed other candidates during the Democratic primaries, to support Obama. Indeed, the Washington Post asserted that union members played a ‘pivotal role’ in Obama’s victory, especially in terms of delivering the white vote. It was a conclusion largely supported by exit polls, which showed that white union members were much more likely to support Obama than whites who were not in unions. The article highlights that despite the decline in union density – by this time only about 12% of American workers belonged to unions, compared to 35% in the 1950s – the labor movement retained considerable political influence, chiefly because of reforms carried out by AFL–CIO President John J. Sweeney. While Obama was unable to fulfill many of the expectations generated by his campaign, the story of labor and the 2008 election is an important one in its own right, showing that contemporary labor could still be a powerful and constructive force.     

Can my religion influence my conception of justice? Political liberalism and the role of comprehensive doctrines

In his last works, John Rawls explicitly argued for an overlapping consensus on a family of reasonable liberal political conceptions of justice, rather than just one. This ‘Deep Version’ of political liberalism opens up new questions about the relationship between citizens’ political conceptions, from which they must draw and offer public reasons in their political advocacy, and their comprehensive doctrines. These questions centre on whether a reasonable citizen’s choice of political conception can be influenced by her comprehensive doctrine. In this paper I present two models of the relationship, which give contrasting answers to these questions, and defend the model that is more permissive with regard to the influence of comprehensive doctrines. This has important implications for our understanding of Rawlsian political liberalism, and reduces the force of objections that have been offered by theorists sympathetic to religion.     

广东鸽源禽Ⅰ型副黏病毒的分子生物学特征

为了解广东地区鸽源禽Ⅰ型副黏病毒的分子生物学特征,选择从该地区分离到的4株鸽源禽Ⅰ型副黏病毒(SD-55株、JM-11株、SZ-14株、ZQ-17株)进行了F基因的扩增及序列测定。结果表明,这4株病毒的F蛋白裂解位点均符合禽Ⅰ型副黏病毒强毒株裂解位点氨基酸序列的分子特征,与中国大陆鸽源APMV-1分离株Pigeon/YN-P1相比,相似率在95%以上。将其与GenBank中的18株鸽源禽Ⅰ型副黏病毒的相应序列进行比较分析,并绘制系统进化树,发现分离株SD-55株、JM-11株、SZ-14株位于以意大利分离株Pigeon/Italy/1166/00为代表的欧洲进化分支,而ZQ-17株位于以美国分离株Pigeon/U.S.(TX)/98为代表的美洲进化分支,经鉴定以上4株病毒均为基因Ⅵb1型禽Ⅰ型副黏病毒。     

羊口疮病毒F1L基因的克隆及其B细胞表位预测

为了研究羊口疮病毒(ORFV)F1L基因,以该病毒基因组DNA为模板,应用聚合酶链式反应(PCR)扩增得到1011bp的F1L基因,将其插入pMD18-TEasy克隆载体,构建了F1L基因克隆重组质粒;再将该基因片段插入pGEX-6P-1表达载体,通过PCR、限制性内切酶双酶切和测序鉴定,得到F1L基因插入方向和读码框结构均正确的阳性质粒,表明,成功构建了羊口疮病毒F1L基因的重组表达载体。通过对F1L基因序列进行生物信息学分析,预测发现F1L蛋白亲水、无信号肽,B细胞表位主要位于第4～8位、第16～22位、第32～53位、第59～73位、第82～99位和第123～133位氨基酸。这将为建立ORFV诊断方法、制备单克隆抗体及合成肽疫苗奠定基础。     

致猪水肿病大肠杆菌标准菌株F107/86 fedA基因的克隆与原核表达

以致猪水肿病大肠杆菌标准菌株F107/86的基因组DNA为模板,对去掉信号肽的456bp的fe-dA基因进行了PCR扩增,将其克隆到原核表达载体pGEX-6P-1的BamHⅠ与EcoRⅠ位点之间,经酶切鉴定与测序,在大肠杆菌中以IPTG诱导表达,并对表达产物用SDS-PAGE和Western-blot分析进行验证。结果表明,筛选出的阳性质粒pGEX-fedA-2(pfedA)经测序并与GenBank中收录的M61713序列进行比较,证明插入序列及读码框正确;表达产物GST-FedA经SDS-PAGE分析大小约42.4ku,Western-blot分析证实其具有良好的反应原性。所构建的原核表达载体可大量表达抗原蛋白,为进一步研究猪水肿病亚单位疫苗奠定了基础。     

罗尔斯的正义原则及其平等观

罗尔斯的正义理论的核心是两个正义原则,第一个是平等自由原则,第二个是机会的公正平等原则与差别原则的结合。两者之间以“词典式”次序排列,并且都蕴含着丰富的平等观念,从而完成了西方政治哲学主题由自由向平等的转换。