应用RNA分析技术推断现场血痕形成时间

目的检测血痕中β-actin mRNA和18S rRNA在人体死后8～15d期间的表达残留,为推断血痕形成时间寻找新的客观依据。方法在上述时段内每天抽提血痕总RNA,利用实时定量RT-PCR技术监测18S rRNA和β-actin mRNA的扩增状况并对产物进行定量分析,通过检测18S rRNA与β-actin mRNA含量在各个时间点的变化趋势来推测血痕形成时间。结果死后8～15 d时段内18S rRNA与β-actinmRNA量的比值呈明显升高趋势,显示出两种不同类型RNA降解的时间差异性。结论一定时段内18SrRNA与β-actin mRNA量的相对变化,可作为推测血痕形成时间的参考指标。     

Molecular Identification of Three Indian Snake Species Using Simple PCR–RFLP Method*

Abstract: Three endangered Indian snake species, Python molurus, Naja naja, and Xenochrophis piscator are known to be significantly involved in illegal trade. Effective authentication of species is required to curb this illegal trade. In the absence of morphological features, molecular identification techniques hold promise to address the issue of species identification. We present an effective PCR–restriction fragment length polymorphism method for easy identification of the three endangered snake species, Python molurus, Naja naja, and Xenochrophis piscator. A 431‐bp amplicon from cytochrome b gene was amplified using novel snake‐specific primers following restriction digestion with enzymes Mbo II and Fok I. The species‐specific reference fragment patterns were obtained for the target species, which enabled successful identification of even highly degraded shed skin sample confirming the utility of the technique in case of poor‐quality DNA. The assay could be effectively used for forensic authentication of three Indian snake species and would help strengthen conservation efforts.     

浅谈我国零售业现代物流信息化建设

随着全球经济一体化的发展,中国零售行业市场的逐渐对外放开,我国零售业想要有立足之地就必然需要加快物流信息化建设的步伐。对问题分析的一个思路是,先就物流信息化的相关概念进行简要界定,之后通过将国内连锁业百强首位的联华超市物流信息化建设现状与国外零售巨头沃尔玛进行分析与比较,最后指出我国零售业物流信息化方面存在的问题并提出加快信息化建设的建议。     

Simultaneous Identification of Four “Legal High” Plant Species in a Multiplex PCR High‐Resolution Melt Assay,

The international prevalence of “legal high” drugs necessitates the development of a method for their detection and identification. Herein, we describe the development and validation of a tetraplex multiplex real‐time polymerase chain reaction (PCR) assay used to simultaneously identify morning glory, jimson weed, Hawaiian woodrose, and marijuana detected by high‐resolution melt using LCGreen Plus^®. The PCR assay was evaluated based on the following: (i) specificity and selectivity—primers were tested on DNA extracted from 30 species and simulated forensic samples, (ii) sensitivity—serial dilutions of the target DNA were prepared, and (iii) reproducibility and reliability—sample replicates were tested and remelted on different days. The assay is ideal for cases in which inexpensive assays are needed to quickly detect and identify trace biological material present on drug paraphernalia that is too compromised for botanical microscopic identification and for which analysts are unfamiliar with the morphology of the emerging “legal high” species.     

基层导向的党政干部培养选拔链研究

近年来,中央加快干部人事制度改革的步伐,通过基层就业、一线招考、公开遴选、双向交流、加强管理考评和监督等手段,不断强化基层导向,使党政干部培养选拔链具有了更多的基层元素和时代特色。进一步规范和完善这条党政干部培养选拔链,需进一步明确链的价值取向、规范链的运行程序、优化链的运行环境、提高链的运行功效。     

Development of a PCR High‐Resolution Melt Assay for Artemisia absinthium (Wormwood) and a Triplex Assay with Two Additional “Unregulated Legal High” Species Datura stramonium (Jimson Weed) and Merremia tuberosa (Hawaiian Woodrose),

Artemisia absinthium (wormwood), a common ingredient in absinthe, contains the compound thujone, which is unregulated by the U.S. Drug Enforcement Agency. Thujone can cause an “unregulated legal high” in higher concentrations. The European Union limits thujone from Artemisia species to 35 mg/kg while the U.S. Food and Drug Administration requires less than 10 ppm to be “thujone‐free.” However, individuals can smoke or ingest A. absinthium in different forms. This study developed a polymerase chain reaction (PCR) high‐resolution melt (HRM) assay to detect and identify A. absinthium based on primer specificity, sensitivity, repeatability, and robustness. A triplex assay was performed with three “unregulated legal high” species: Datura stramonium, Merremia tuberosa, and A. absinthium; the PCR HRM assay detected and identified each plant at melt temperatures 77.42 ± 0.20°C, 83.88 ± 0.22°C, and 87.77 ± 0.15°C, respectively. The primer set developed distinguished A. absinthium from a variety of plant species and was successfully triplexed.     

农业高新技术与涉农供应链基本战略的实现

受内在的诸多固有问题影响,传统涉农供应链无论采取总成本领先战略还是差异化战略都面临着重大障碍。21世纪农业高新技术的发展为涉农供应链提供了战略性再造的契机。它能够营造涉农链的高度稳定,进而强力支持涉农链基本竞争战略的实现和深化。     

温州汉族人群D7S2846、D19S400和D18S535基因座的遗传多态性研究

目的研究D7S2846、D19S400和D18S535位点在温州汉族人群的遗传多态性。方法EDTA抗凝血样采自194名无血缘关系温州地区汉族个体,用chelex-100法提取DNA,PCR扩增,聚丙烯酰胺凝胶电泳,银染显色分析。结果D7S2846观察到6个等位基因及15种基因型;D19S400观察到10个等位基因及36种基因型;D18S535观察到8个等位基因及26种基因型。各基因座的杂合度(H)分别为:0.644、0.724、0.772;个人识别能力(Dp):0.854、0.940、0.938。结论三个STR基因座具有较高杂合度,等位基因分布符合Hardy-Weinberg平衡,在法医学应用和群体遗传学研究中有较高的价值。     

现代管理思想与现代物流管理

在供应链与供应链之间竞争取代单个企业之间竞争的时代 ,作为供应链管理的重要组成部分的现代物流管理和传统的物流管理相比发生了巨大的变化。业务流程重组思想、快速反应思想和关注价值链的战略思想 ,分别是现代物流管理的组织、方法和效益的集成和优化的思想基础     

供应链管理在市场营销中的作用

在市场营销中,建立科学的合理的高效的物流供应链,可以提高企业客户服务水平,大幅降低物流成本;同时,加强和优化供应链管理,还能创造新的市场需求,为顾客创造更多价值;供应链管理还可解决网络营销的瓶颈,使网络营销效果倍增。供应链管理已成为企业市场营销成功与否的关键一环。