HE染色涂片上精子DNA的提取与HLA-DQa基因扩增分型

本文对ＨＥ染色涂片上精子ＤＮＡ的提取、ＰＣＲ扩增分型的可行性与可靠性进行了研究。２０张ＨＥ染色涂片的实验结果表明：ＨＥ染色涂片上的精子是可以被回收并提取到ＤＮＡ进行ＨＬＡ－ＤＱａ基因的ＰＣＲ扩增分型的，其分型结果准确可靠。这说明精子的ＨＥ染色涂片也是进行ＤＮＡ分析的一种很好的检材来源。     

略论邓小平理论萌芽的界定及其标志

作为邓小平理论萌芽的标志不是某一具体的时间、论著、观点。对邓小平理论萌芽应作出系统的、全面的科学界定。邓小平理论萌芽的标志应该是时间段、论著链、观点网。     

用引物Y_3、Y_4和PCR方法鉴定性别的法医学应用

用Y3、Y4和Alu9．1、Alug．2两对引物和PCR方法检测陈旧血痕和毛根的性别获得成功。引物Y3、Y4扩增的靶序列位于Y染色体特异3．4Kb重复序列中，扩增产物为460bp；引物Alu9．1、Alu9.2用以扩增男女共有的Alu重复序列，扩增产物为130bP。室温保存13年之久的19例脐带血血痕（男性9冽，女性10例）和室温保存10～11个月的10例已知性别自然脱落毛根（男性6例，女性4例）的性别测定结果均正确；对一起凶杀案的血痕性别测定为定案提供了重要证据。本方法简化了样品的前处理过程。     

日本强震及核危机对世界经济的影响分析

地震、海啸、核安全问题接连发生,日本正在经历史上罕见的"大创伤"。强震、海啸、核危机对日本经济的负面冲击明显大于阪神地震。日本地震后的灾后重建与阪神地震时的财政环境明显不同,地震提高了日本政府的违约风险和隐含的主权债务风险。但对世界经济整体而言,日本大地震不会影响全球经济复苏的步伐。虽然全球股市企稳回升,但"核阴云"笼罩亚太、大宗商品呈现先抑后扬的态势,加之受利比亚战争及发达国家的量化宽松货币政策等外围因素影响,日本大地震使全球金融市场未来面临诸多不确定性。日本地震对中国经济影响有限,灾后重建对我国出口及产业发展带来有利契机。     

A new strategy for the discrimination of mitochondrial DNA haplogroups in Han population

Mitochondrial DNA (mtDNA) haplogroup discrimination is interesting not only for phylogenetic and clinical but also for forensic studies. We discriminated the mtDNA haplogroups of 570 healthy unrelated Han people from Zhejiang Province, Southeast China, by comprehensive analysis mutations of the hypervariable segments-I sequence and diagnostic polymorphisms in mtDNA coding region using real-time polymerase chain reaction (RT-PCR), which was compared with the widely used PCR and restriction fragment length polymorphism (PCR-RFLP) method. The results showed that in superhaplogroup M, haplogroup D was the most common haplotype within this assay to 24.6%, and in the other superhaplotype N, haplogroup B and F were the most common groups. Samples re-identified by PCR-RFLP showed the consistent results that were got with RT-PCR. In conclusion, the RT-PCR strategy appears to be an accurate, reproducible, and sensitive technique for the discrimination of mtDNA haplogroups, especially for mass screenings quickly and economically.     

An Investigation of PCR Inhibition Using Plexor®‐Based Quantitative PCR and Short Tandem Repeat Amplification

A common problem in forensic DNA typing is PCR inhibition resulting in allele dropout and peak imbalance. In this paper, we have utilized the Plexor^® real‐time PCR quantification kit to evaluate PCR inhibition. This is performed by adding increasing concentrations of various inhibitors and evaluating changes in melt curves and PCR amplification efficiencies. Inhibitors examined included calcium, humic acid, collagen, phenol, tannic acid, hematin, melanin, urea, bile salts, EDTA, and guanidinium thiocyanate. Results were plotted and modeled using mathematical simulations. In general, we found that PCR inhibitors that bind DNA affect melt curves and C_T takeoff points while those that affect the Taq polymerase tend to affect the slope of the amplification curve. Mixed mode effects were also visible. Quantitative PCR results were then compared with subsequent STR amplification using the PowerPlex^® 16 HS System. The overall results demonstrate that real‐time PCR can be an effective method to evaluate PCR inhibition and predict its effects on subsequent STR amplifications.     

α-纤维蛋白原基因多态性与肺动脉栓塞相关性初探

目的探讨α-纤维蛋白原Thr312Ala基因的多态性分布与肺动脉栓塞的相关性。方法选取尸检已确诊的肺动脉栓塞病例的甲醛固定心肌组织14例和正常健康人群外周血56例,采用改良酚-氯仿法和Chelex100法分别提取心肌组织和外周静脉血基因组DNA,用PCR-RFLP法检测α-纤维蛋白原基因Thr312Ala多态性,并分析其与肺动脉栓塞的关联性。结果α-纤维蛋白原Thr312Ala基因的各基因型在肺动脉栓塞组与对照组比较差异具有显著性意义（P〈0.05）;各等位基因在两组间比较差异具有显著性意义（P〈0.05）;A等位基因与PE呈负相关（RR=0.475,P〈0.05）;G等位基因与PE呈正相关（RR=5.818,P〈0.05）。结论α-纤维蛋白原基因Thr312Ala位点G等位基因与肺动脉栓塞有正关联,肺动脉栓塞死亡者中GA、GG基因型分布频率较高,本文数据可为相关研究提供参考。     

Modified Midi‐ and Mini‐Multiplex PCR Systems for Mitochondrial DNA Control Region Sequence Analysis in Degraded Samples

Two multiplex polymerase chain reaction (PCR) systems (Midiplex and Miniplex) were developed for the amplification of the mitochondrial DNA (mtDNA) control region, and the efficiencies of the multiplexes for amplifying degraded DNA were validated using old skeletal remains. The Midiplex system consisted of two multiplex PCRs to amplify six overlapping amplicons ranging in length from 227 to 267 bp. The Miniplex system consisted of three multiplex PCRs to amplify 10 overlapping short amplicons ranging in length from 142 to 185 bp. Most mtDNA control region sequences of several 60‐year‐old and 400–500‐year‐old skeletal remains were successfully obtained using both PCR systems and consistent with those previously obtained by monoplex amplification. The multiplex system consisting of smaller amplicons is effective for mtDNA sequence analyses of ancient and forensic degraded samples, saving time, cost, and the amount of DNA sample consumed during analysis.     

农业产业化重点企业涉农供应链的实践与启示

从国家级农业产业化重点"龙头"企业供应链实践的基本状况和特点中,可以得到如下启示:强化管理,夯实基础;大力推进和巩固农业产业化经营;基于SCM的协作式竞争理念的觉醒;IT界应加强集成化涉农供应链研发的力度和针对性;高度重视农业高新技术在涉农供应链中的运用;借鉴西方经验,加强宏观关注和政策支持。