成都地区汉族人群D2S441位点的遗传多态性研究

为研究 STR位点 D2S441的遗传多态性,为法医学应用提供基础数据,应用 PCR及 PAG电泳技术对 260名成都地区汉族无关个体进行了调查,共检出 9个等位基因及 26种基因型,首次获得汉族群体频率分布 ,其等位基因片段大小范围为 131～ 155bp。该位点基因型频率分布符合 Hardy－ Weinberg平衡。家系调查证实了等位基因的传递遵循孟德尔遗传规律。其个人识别能力（ Dp）、杂合度（ H）、多态性信息含量（ PIC）和非父排除率（ PE）分别为 0.9084、 0.7885、 0.7390和 0.5778,表明该位点在法医学个人识别及亲子鉴定中具有较高的实用价值。     

中国北方汉族人群HLA-DQα基因型及其等位基因频率的分布

应用PCR和等位基因特异性寡该苷酸（ASO）探针杂交技术对中国北方汉族人群的HLA－DQα基因进行分型。在246例无关个体中观察到4种等位基因组成的10种基因型。等位基因频率分布在10．0～31．9％之间。基因型的分布符合Hardy－Weinberg定律。DP值为0．8669。对6个家系49个个体的调查表明，HLA－DQα基因按孟德尔方式遗传。不同人群HLA－DQα的DP值比较，中国人群高于其它人群。     

企业实施绿色供应链管理的社会环境探讨

市场竞争的加剧导致了资源枯竭、环境恶化，人们越来越关注可持续发展，绿色供应链管理成为提高资源利用水平、环境保护的有效手段。该文从政府绿色政策、市场绿色环境、企业绿色文化、社会绿色服务体系等多方面分析了企业实施绿色供应链管理所需要的社会环境，并提出相关建议。     

特许连锁经营法律关系初探

特许连锁经营是以特许经营权的转让为核心的一种经营方式。文章重在分析和研究特许连锁经营法律关系的性质,以及作为这一法律关系客体的特许经营权的法律属性。     

The Structure of Material Evidence Authentication Rules

The authentication rules of material evidence is very important for justice. The core of authentication system of real evidence is storage chain rules. We can only accept the evidence in the condition of legally custodian, evidence collected under legal procedure, the transfer without delay, the transport under control, the suitable environment. The authentication system of real evidence also contents the exception of storage chain rules.     

Hardening corporate accountability in commodity supply chains under the European Union Deforestation Regulation

The European Union (EU) has recently introduced the Deforestation Regulation to close regulatory gaps in the sustainability and legality of global forest and agricultural commodity supply chains. We analyze this regulatory policy change by drawing on accountability scholarship and institutionalist theories of regulation. Our results show that the Regulation aims to enhance corporate accountability mechanisms through mostly state-based hard regulation of commodity supply chains, reducing the role of market incentives and private regulation. This policy change is found to be the result of strategic policy-oriented learning from perceived accountability failures of existing soft market-based instruments, voluntary trade agreements, and experience with market-correcting EU timber legality trade rules in a politically favorable context. The institutionalization of new forest-risk commodity supply chain accountability norms in new EU trade rules would, by design, harden foreign corporate accountability for negative socio-environmental externalities. However, the de-facto hardening will depend on the final regulatory design, acceptance, compliance, implementation, enforcement improvements, and avoidance of leakage effects.     

多点连环式恐怖袭击的防范策略

多点连环式恐怖袭击的袭击目标多、影响范围大,是需要加以重视的一种恐怖袭击类型。通过对GTD数据进行统计分析,指出该类恐怖袭击发案区域广泛主要聚焦于中东和北非等地区,其特点是目标明确、多为团伙作案且使用炸弹袭击方式。文章结合“蜂巢分析法”分析作案模式,总结发案原因,提出加强安防能力、完善情报工作流程的工作措施,以供实战部门进行针对性防控。     

STR Profiles from DNA Samples with "Undetected" or Low Quantifiler™ Results

Abstract:  Screening methods capable of identifying DNA samples that will not yield short tandem repeat (STR) profiles are desired. In the past, quantitation methods have not been sensitive enough for this purpose. In this study, low level DNA samples were used to assess whether Quantifiler™ has a minimum quantitation value below which STR profiles would consistently fail to be detected. Buccal swabs were obtained and the DNA extracted, quantified, and serially diluted to concentrations ranging from 0.002 to 0.250 ng/μL. Samples were analyzed once with Quantifiler™, followed by Profiler Plus™ amplification and capillary electrophoresis analysis. An absolute minimum value below which STR results were unobtainable could not be defined. From the 96 low level samples tested, STR loci (including one full profile) were successfully amplified and detected from 27% of the samples "undetected" by Quantifiler™. However, no STR alleles were detected in 73% of these "undetected" samples, indicating that Quantifiler™ data may be useful for predicting STR typing success.     

Performance Testing of a Semi-Automatic Card Punch System,Using Direct STR Profiling of DNA from Blood Samples on FTA™ Cards

The 1.2 mm Electric Coring Tool (e-Core™) was developed to increase the throughput of FTA^™ sample collection cards used during forensic workflows and is similar to a 1.2 mm Harris manual micro-punch for sampling dried blood spots. Direct short tandem repeat (STR) DNA profiling was used to compare samples taken by the e-Core tool with those taken by the manual micro-punch. The performance of the e-Core device was evaluated using a commercially available PowerPlex™ 18D STR System. In addition, an analysis was performed that investigated the potential carryover of DNA via the e-Core punch from one FTA disc to another. This contamination study was carried out using Applied Biosystems AmpflSTR™ Identifiler™ Direct PCR Amplification kits. The e-Core instrument does not contaminate FTA discs when a cleaning punch is used following excision of discs containing samples and generates STR profiles that are comparable to those generated by the manual micro-punch.     

Medium‐Chain Acyl‐CoA Dehydrogenase Deficiency in Adulthood: A Potential Diagnosis in a Patient with Mental Status Changes Suspected of Drug Toxicity

Medium‐chain acyl‐CoA dehydrogenase (MCAD) deficiency is a rare but important component of the differential diagnosis for adults with a history of premortem mental status changes and the postmortem finding of hepatic steatosis. This case report describes a 30‐year‐old white man who, following a period of nausea and vomiting, was admitted to the hospital with sudden mental status deterioration followed rapidly by clinical deterioration and death. Treating physicians in this case suspected acute illicit drug toxicity with synthetic cathinones based on social history. Clinicians and medical examiners should be aware that the presentation, signs, and symptoms described may indicate an underlying inborn error of metabolism such as MCAD deficiency and take action accordingly.