Validation of the InnoXtract™ DNA extraction method for bone,teeth, and rootless hair

Forensic casework samples often include human hairs, teeth, and bones. Hairs with roots are routinely processed for DNA analysis, while rootless hairs are either not tested or processed using mitochondrial DNA. Bones and teeth are submitted for human remains identifications for missing persons and mass disaster cases. DNA extraction from these low templates and degraded samples is challenging. The new InnoXtract DNA extraction method utilizes magnetic beads that are optimized to bind small DNA fragments, as small as 100 base pairs, to purify high-yield DNA from compromised samples. This validation study evaluates InnoXtract's ability to obtain amplifiable DNA from samples such as rootless hairs and skeletal remains. Studies performed include sensitivity, stability, repeatability, reproducibility, non-probative samples, and comparison to standard organic extractions. Sensitivity studies demonstrate average yield recoveries ranging from 53% to 100% and 73% to 85% for the InnoXtract hair and bone methods, respectively. Studies demonstrate consistent results across a range of sample types, such as insulted and un-insulted bone and teeth, as well as hair shafts from donors of various ages, gender, race, and hair characteristics. The InnoXtract bone method outperformed organic extraction. The method was successfully automated on a MagMAX™ Express-96, with recoveries over 70% relative to the manual version. InnoXtract has the potential as an automated high-throughput, high-yield bone extraction method with 6 h of total extraction time for up to 96 samples. The validation study results demonstrate that the InnoXtract kits produce high-yield and high-quality DNA from compromised bone, teeth, and hair shaft samples.     

Disowning Fukushima: Managing the credibility of nuclear reliability assessment in the wake of disaster

This paper reflects on the credibility of nuclear risk assessment in the wake of the 2011 Fukushima meltdowns. In democratic states, policymaking around nuclear energy has long been premised on an understanding that experts can objectively and accurately calculate the probability of catastrophic accidents. Yet the Fukushima disaster lends credence to the substantial body of social science research that suggests such calculations are fundamentally unworkable. Nevertheless, the credibility of these assessments appears to have survived the disaster, just as it has resisted the evidence of previous nuclear accidents. This paper looks at why. It argues that public narratives of the Fukushima disaster invariably frame it in ways that allow risk‐assessment experts to “disown” it. It concludes that although these narratives are both rhetorically compelling and highly consequential to the governance of nuclear power, they are not entirely credible.     

OVM Wins the Bid for a Nuclear Power Research Project

正The Liu zhou OVM Machinery Co.Ltd.(OVM)recently won the bid offered by China Nuclear Power Engineering C o.L t d.f o r t h e research and development of a steel joint used in the shell of a third-generation nuclear power plant that protects the plant from being hit by big commercial planes.Ever since the Fukushima Daiichi nuclear disaster which occurred at the Fukushima I Nuclear Power Plant on March 11,2011,countries around the world have improved the safety standards for nuclear power plants.In China,another shell is built around the containment     

Changes in the Frequency and Clinical Features of Suicide Attempts in the Midwestern Area of Kanagawa after the Great East Japan Earthquake

The aim of this study was to investigate the relationship between the clinical features of suicide attempts and the Great East Japan earthquake in Kanagawa. We enrolled 592 patients who attempted suicide and were hospitalized for inpatient treatment. Clinical features were compared between before the earthquake (A) and after the earthquake (B) groups. The number of suicide attempts increased from 286 to 306. The rate of increase in suicide attempts in men was the highest in the age group of 40–59 years. The frequency of joblessness, family psychiatric histories, precipitating event, and alcohol intake at suicide attempt was significantly higher in the B group than in the A group. The number of patients in serious condition in the B group was significantly higher than that in the A group. Other clinical features related to suicide in people who were indirectly affected by the earthquake disaster should be investigated to develop targeted prevention strategies.     

On the use of play theory in analyses of online public commentary

Abstract

The concept of play mediates between deliberation as a mode of reason and resistance as a mode of culture, thus opening a way to think about hostile comment (e.g., ‘flaming’) on online news forums as normal patterns of behaviour, instead of a departure from the received view of how citizens ought to consider matters of public interest. The play concept corresponds with current thinking around the notion of cultural citizenship. To illustrate the relevance of play theory in the analysis of online political discourse, this article uses an example from recent posts concerning the Protection of Information Bill (POIB) in the online site of the South African Mail & Guardian newspaper. The cogency of play theory to the concept of citizenship is argued through a discussion of how citizenship has been understood from the 1930s to the late 1980s and early 1990s, when the improved capacities of information and communications technologies (ICTs) made online deliberation a normal site for citizenship to be exercised.     

Milton's army: The white house press corps

White House reporters follow a path constructed by presidential advisers that they hope will lead them to fulfill goals set by their news organizations. White House officials ration them facilities for work, access to newsworthy people, and reportable information in amounts that depend on the importance to the President of the type of media they work in, the status of their particular news organization, and the staff's respect for the influence and competence of a particular individual. In this context, several constraints that affect White House reporting are discussed here: those placed on reporters by their organizations; by the way their type of media covers the White House; by their relations with each other; and by their concepts of what they are required to do. The framework for this discussion and analysis is a classification by type of media and news organizations that assign journalists to the White House. Of the resulting six categories, the first three have the most structural and organizational influence and are given the most attention. The special status, unique history, and influence of photographers at the White House require that they be treated separately.     

Crime News Trends in Finland: A Review of Recent Research

In this article, we present findings of crime media research conducted in Finland during recent years. As this body of research has mainly been published in Finnish, the core results have not been available for international audiences until now. The amount of crime news reporting has consistently increased both in the newspapers and in television news broadcasts during the last decades. A series of representative surveys also show that the majority of people read and view crime news on a regular basis and are thus constantly exposed to these media messages. We conclude our article by contrasting crime news trends with the development of fear of crime in Finland, and by discussing possible links and explanations to the relationship.     

Depictions of correctional officers in newspaper media: an ethnographic content analysis

The public relies on the media for most of its information about the criminal justice system. Unfortunately, media depictions of justice actors are not always accurate which, in turn, can lead to distorted images about the system and its operations. Using ethnographic content analysis to analyze 489 articles from major newspapers across the United States, this study seeks to discern how correctional officers and the jobs that they perform are portrayed in print media. The results suggest that correctional officers are overwhelmingly portrayed negatively, with 79.6% of the articles in the research sample presenting one of six distinct negative themes. A typology of these themes is explored in detail, along with its implications for societal support for corrections and correctional officers, especially with regard to the media’s potential contributions to officers’ job stress, burnout, and job dissatisfaction.     

Across time and space: Explaining variation in news coverage of the European Union

News about the European Union (EU) looks different in different countries at different points in time. This study investigates explanations for cross‐national and over‐time variation in news media coverage of EU affairs drawing on large‐scale media content analyses of newspapers and television news in the EU‐15 (1999), EU‐25 (2004) and EU‐27 (2009) in relation to European Parliament (EP) elections. The analyses focus in particular on explanatory factors pertaining to media characteristics and the political elites. Results show that national elites play an important role for the coverage of EU matters during EP election campaigns. The more strongly national parties are divided about the EU in combination with overall more negative positions towards the EU, the more visible the news. Also, increases in EU news visibility from one election to the next and the Europeanness of the news are determined by a country's elite positions. The findings are discussed in light of the EU's alleged communication deficit.     

News Norms and the Strategic Timing and Content of Candidate Messages

Abstract

In this paper, we ask what sets of factors influence the timing and the content of presidential candidate press releases. Utilizing a unique data set, we examine the influence of internal campaign factors as well as external media factors on campaign information dissemination during the invisible primary season. We find that both types of factors may influence the timing of press releases; as to content, however, media norms are less likely to alter a candidate's general strategic message, which is dictated by his competitive status and national stature.