表达狂犬病病毒CVS-11株糖蛋白的重组伪狂犬病病毒在犬体内的免疫效果

为研制一种新型有效的狂犬病重组活载体疫苗,将EGFP-rgp的表达框克隆入p8-AA载体的Sph Ⅰ/Nde Ⅰ酶切位点处,将酶切鉴定为正向连接的阳性重组子命名为p8AA-EGFP-rgp.在质脂体介导下将该质粒与Bartha-K61共转染PK-15细胞,获得能够表达狂犬病病毒(RV)CVS-11株糖蛋白的重组伪狂犬病病毒rPRV/EGFP-rgp,经RT-PCR和Western-blot鉴定后对犬进行肌肉注射免疫,并测定相应试验指标,研究重组病毒对犬的免疫诱导情况.结果显示,重组病毒可以有效地刺激机体产生体液免疫和细胞免疫;产生的针对RV的中和抗体水平[(0.95±0.21)U/mL]在14周时还保持相对国际最低保护标准(0.5U/mL)较高的水平;且产生的针对RV和PRV的中和抗体水平均达到了相应灭活疫苗和减毒活疫苗的水平,抗体效价均维持在14周以上.     

Biomarket for transforming agriculture to drive Asian economic boom

Asian countries have historically faced challenges of higher population growth and a traditional economy largely based on primary production. Even with good potential for agriculture in terms of available area, basic infrastructure and social skills, plant diseases, poor yielding crop varieties, irregular rainfall and other environmental factors keep the produce much lower. The paper suggests the concept of biomarket as a combination of biotechnology and economy. Notwithstanding public apprehensions, biotechnology promises environment and infection resistant crop varieties, higher yield, improved taste, texture and nutrition of foods, independence from fertilizers and pesticides. However, there is time lag between the research and implementation not to mention the increasing costs involved. A biomarket then would start with a consortium of scientists from member countries pooling research tracks and knowledge of otherwise locally available/developed biotechnologies and making them easily available to all members. Exim policies, IPRs and transparent works would have to be modeled to the opening and sharing of research and its use in the fields and homes. Technology transfer, industry-academic partnership, identification and marketing of bioproducts, international centres, and utilization of indigenous technologies in modern way would streamline the operation of biomarket across Asia. Biomarket will be more evolved than the conventional markets by including knowledge based economy. Social awareness of various biotechnologies and demonstration of success in implementing them locally would engage people＇s life styles. This will ensure wider sociological impact with a purpose of economic progress. Biomarket will finally aim for Asia to emerge as a world power bloc.     

当代国际贸易发展的特点探析

当代国际贸易的发展呈现出以下特点和趋势：贸易商品结构上日趋高级化;贸易格局上全球化与集团化并存;绿色浪潮赋予国际贸易新的涵义,绿色贸易大行其道;贸易手段上,网络贸易将成为主导性贸易方式;贸易政策上,管理贸易盛行;技术壁垒成为贸易保护主义的新策略。     

树立环境育人理念 建设绿色大学——以清华大学的改革措施为例

高校的根本任务是培养人,一切工作都应紧紧围绕这个中心来开展。按照科学发展观的要求,高校的中心工作应体现育人为本的理念。除了开展传统的教书育人、管理育人和服务育人外,许多教育工作者越来越重视校园环境在育人中的重要作用,认为它是学校的第四种育人方式。也就是说,一个良好的校园环境不仅能够对学生起到潜移默化的作用,而且对教书、管理和服务育人工作的开展和实施也能起到推动和促进作用。     

绿色标志使用与监督行为的博弈论模型研究

绿色标志的监督认证是政府运用行政手段监督管理绿色产品,保障消费者和企业合法权益的重要手段。本文运用博弈论这一经济学研究工具,探讨政府如何高效地监督绿色标志的使用情况,打击违法使用绿色标志损害人民利益的行为,以期得出政府监督管理绿色标志使用行为的最优策略。     

政府绿色管理：政府管理发展的新思路

作为一种管理新理念，绿色管理不仅指引企业健康发展，而且在一定程度上促进政府管理创新。我国政府也在积极探索政府绿色管理的路径，并取得了一定的成就，但是还存在很大的局限性。要完善政府绿色管理，就要建立绿色管理的体制机制，建立绿色GDP核算标准，同时健全相关的绿色法律法规体系，提高政府的执政能力。     

中国环境治理的主要成效与思路研究

改革开放以来,中国环境治理理念日益明确,环境治理成效显著增强,环境治理法制不断完善,环境治理机构不断健全。但中国在环境治理能力、治理机制、治理法律、治理手段等方面仍然面临着挑战。需要借鉴国外经验,从环境立法执法、市场工具运用、发展绿色经济、提升治理科技水平和扩大公众参与等方面加强环境治理。     

The Integration of Best Environmental Management Practices in Corporate Strategy: Empirical Evidence From Italian Local Governments

Local governments represent an important moving force in promoting sustainable development policies as they make up the level of governance closest to the citizens. The aim of this article is to examine whether Italian local governments exercise de facto their vital role in fostering sustainable development policies. In particular, we analyzed municipality behavior about adopting “green management strategies.” We investigated the main factors identified in management literature which might boost the adoption of these strategies. Several implications ensue from our findings. In particular, the research provides information to improve local government attitudes toward the sustainable development challenge.     

坚持科学发展观实现中国特色的建筑节能

该文立足中国国情，借鉴国外建筑节能发展的实践经验，总结了中国建筑节能工作存在的问题，从坚持科学发展观的角度，提出了实现中国特色建筑节能目标的有效途径。     

我国绿色房地产发展的理念与策略

理性增长、3D发展、公平为上和绿色繁荣构成了城市房地产的绿色理念与目标。应该设定协同的行动领域，首先在经济、社会、建设、资源环境和绿色发展的能力等领域取得重点突破；同时，要以体制变革促进发展，实现发展系统的整体优化。实施绿色机制对政府的政策能力提出了更高的要求，也需要更多的共识与共享。减资源型的房地产经济体系是房地产绿色发展的基本保证。当前，房地产调控应该明确地把绿色发展的理念融入其中。