3种特殊染色在急性心肌梗死死后诊断中的应用比较

目的对比观察苏木素-碱性复红-苦味酸染色(HBFP染色)、变色酸2R-亮绿染色和Heidenhain染色在急性心肌梗死死后诊断中的应用价值。方法以大鼠急性心肌梗死模型、法医检案急性心肌梗死心脏标本作为研究对象,采用HBFP染色、变色酸2R-亮绿染色和Heidenhain染色进行对比观察。结果①3种特染方法在大鼠心肌缺血15min时均可观察到阳性染色,且阳性染色面积随缺血时间的延长而扩大;②大鼠急性心肌缺血4h心脏标本在-20℃、4℃及室温条件下保存至14d,3种特染方法仍可见阳性着色,但变色酸2R-亮绿染色和HBFP染色随保存时间的延长而出现着色能力下降,阳性区域变小的趋势,Heidenhain染色效果最为稳定;③急性心肌梗死检案标本中,3种特染方法均可显示缺血心肌纤维,发病1h内死亡者Heidenhain染色优于另外两种染色。结论 3种特染均可客观的显示出急性心肌梗死早期病理改变,其中Heidenhain染色更具稳定性和可操作性。     

Uneven development,inequality and concentration of power: a critique of Thailand 4.0

This article provides a critique of the Thailand 4.0 strategy to push the country out of the middle-income trap through innovation-driven, inclusive and sustainable growth. First, it argues that the policies have insufficiently analysed the persistence of structural hierarchy and uneven development in the global political economy, which will constrain Thailand’s catch-up success in the future. Second, based on writings about progressive mission-led industrial strategies, it is argued that Thailand 4.0 ought to embed a progressive social and environmental agenda more clearly in its industrial strategy. Third, it is argued that Thailand 4.0 neglects to address the high concentration of political and economic power in the country, and also continues to allow unequal access to the policymaking process that has led to socio-environmental problems. Overall, this article argues that Thailand 4.0 will increasingly aggravate the two-tier fragmented nature of the political economic system of Thailand, where few can reap the biggest shares of the surplus and participate in more advanced sectors of the economy. It also calls for a more progressive industrial strategy and an alternative developmental path.     

From sustainable development through green growth to sustainable development plus

Abstract

From sustainable development (SD) through green growth to sustainable development plus (SD+)! Does the ‘+’ really matter? This is the question that brings life to this article and the answer is: YES, it does. The ‘+’ makes a huge difference especially for developing countries such as those from Africa that for a long time have not been fully brought on board in shaping global discourses and the preferred future global development agenda. This article argues that the time has come for globally privileged countries of the North to realise that operationalising ‘The Future We Want’ after Rio+20 demands that developing countries be accorded unpolluted space to contribute to providing answers to difficult and elusive questions on the unsustainable ways of the past development paradigms. Among such questions are: When will the issue of resource intensive development and overconsumption be finally answered? Can green growth transition be part of the solution? How will a post 2015 framework best address the needs of developing countries? The conclusion is that SD+ signals a deeper, wider and knowledge-based understanding of global (un) sustainable perspectives that result in global citizens understanding The Future We Do Not Want.     

Innovations in Constructing the Framework of China's Environmental Code —Referencing the Framework of China's Civil Code

The success in compiling China's Civil Code benefits from factors such as China's complete system of civil law statutes, the methodology of the Pandekten System, and the private-law attributes of civil laws, etc. To construct the framework of China's environmental code, it is necessary to reference that success for theoretical and structural innovations. In addition, the fundamental features and inherent issues of China's existing environmental law system, such as its breadth, the extensiveness of origins of environmental law, the multiple implication of environmental legal relations, and the compound nature of the environmental legal liability system, should be taken into account. The layout structure of General—Specifics should be adopted for compiling China's environmental code, while moderate codification and extraction of the common factor (vor die Klammer zu ziehen) for connection with applicable rules of specific laws should serve as construction principles and methods for compiling the framework of China's environmental code. The environmental code should have five parts: General ; Pollution Prevention and Control ; Nature Conservation ; Green and Low-Carbon Development ; and Ecological and Environmental Responsibility.     

Evaluation of six presumptive tests for blood, their specificity, sensitivity, and effect on high molecular-weight DNA

Luminol, leuchomalachite green, phenolphthalein, Hemastix, Hemident, and Bluestar are all used as presumptive tests for blood. In this study, the tests were subjected to dilute blood (from 1:10,000 to 1:10,000,000), many common household substance, and chemicals. Samples were tested for DNA to determine whether the presumptive tests damaged or destroyed DNA. The DNA loci tested were D2S1338 and D19S433. Leuchomalachite green had a sensitivity of 1:10,000, while the remaining tests were able to detect blood to a dilution of 1:100,000. Substances tested include saliva, semen, potato, tomato, tomato sauce, tomato sauce with meat, red onion, red kidney bean, horseradish, 0.1 M ascorbic acid, 5% bleach, 10% cupric sulfate, 10% ferric sulfate, and 10% nickel chloride. Of all the substances tested, not one of the household items reacted with every test; however, the chemicals did. DNA was recovered and amplified from luminol, phenolphthalein, Hemastix, and Bluestar, but not from leuchomalachite green or Hemident.     

Human genomic DNA quantitation system, H-Quant: development and validation for use in forensic casework

The human DNA quantification (H-Quant) system, developed for use in human identification, enables quantitation of human genomic DNA in biological samples. The assay is based on real-time amplification of AluYb8 insertions in hominoid primates. The relatively high copy number of subfamily-specific Alu repeats in the human genome enables quantification of very small amounts of human DNA. The oligonucleotide primers present in H-Quant are specific for human DNA and closely related great apes. During the real-time PCR, the SYBR Green I dye binds to the DNA that is synthesized by the human-specific AluYb8 oligonucleotide primers. The fluorescence of the bound SYBR Green I dye is measured at the end of each PCR cycle. The cycle at which the fluorescence crosses the chosen threshold correlates to the quantity of amplifiable DNA in that sample. The minimal sensitivity of the H-Quant system is 7.6 pg/microL of human DNA. The amplicon generated in the H-Quant assay is 216 bp, which is within the same range of the common amplifiable short tandem repeat (STR) amplicons. This size amplicon enables quantitation of amplifiable DNA as opposed to a quantitation of degraded or nonamplifiable DNA of smaller sizes. Development and validation studies were performed on the 7500 real-time PCR system following the Quality Assurance Standards for Forensic DNA Testing Laboratories.     

基于绿色城市模式的两型社会构建

该文对两型社会中绿色城市模式进行了定位，并分析其对于两型社会构建的重要意义，对绿色城市模式的层次构成、发展策略与运作机制进行了探讨。     

高校“青马工程”培养路径研究——基于对大学生创业教育视角的思考

高校“青马工程”的培养,旨在帮助高校大学生骨干学习和掌握党的理论创新成果,了解国情,认识社会,提高思想政治素质,坚定理想信念.但是,高校“青马工程”的开展,在培养实践过程应该更好的总结经验,采取多层次、立体化、互动学习的培训模式,要立足于高校创业教育的视角,立足于大学生全面成才发展的需要,将理论和实践相融合,设置符合“青马工程”培养需求的创业教育机制,建立“青马工程”创业教育评价标准,从而完善“青马工程”的教育体系.     

论民法研究范式的绿色转向

在转型中国的时代背景下,民法研究范式应该实现绿色转换,即民法研究应该以建构一种人与自然、人与人之间以及人内心整体动态和谐的"绿色"市民社会秩序为根本目标,以包容协作为基本理念,以弱势群体的保护、协作精神的贯彻、利益冲突的疏导作为民事制度建构的基本方向,在民法的内外体系上对传统理论进行革新,从而确立一个新的学术共同体和新的研究范式,来回应生态、人态和心态日益紧张的现代市民社会。