6FAM-HEX-TMR-ROX四色荧光分析体系的构建

目的为在310基因分析仪上进行6FAM-HEX-TMR-ROX四色荧光STR复合扩增分型建立基础条件。方法以pGEM载体作为靶DNA,设计引物扩增获得500~80bp的13个长度不等DNA片段,以这些片段纯化物作为模板DNA,分别扩增获得6FAM、HEX、TMR和ROX标记的M atrix标准物,用4种M atrix标准物在310基因分析仪上构建可用于6FAM-HEX-TMR-ROX四色荧光分析的M atrix文件。结果扩增所得的13个非标记片段,长度分别为80、100、120、140、160、180、200、220、260、300、340、400、500bp,在非变性聚丙烯酰胺凝胶连续电泳-银染凝胶上均表现为单条带,6FAM、HEX、TMR、ROX分别标记的片段通过310基因分析仪检测均为单峰。混合ROX标记的80、120、180、220、260、300bp的6个片段获得的内标,显示出良好的线性关系。结论建立了有效的6FAM-HEX-TMR-ROX四色荧光分析M atrix,为进行多个STR基因座荧光标记复合扩增检测奠定了基础。     

集体腐败的情景预防理论分析

因集体腐败引致损失更大、影响更恶劣、作案更隐蔽使其成为了近期反腐工作中的焦点,集体腐败具有集体腐败主体的潜在性特征、集体腐败行为的被动性特征及集体腐败行为被动性转化的特征,聚焦腐败主体本位是目前集体腐败进行类型划分及其成因探源的主要方式,内因驱动是其主要逻辑起点。然而,忽视外因是这一研究路径的主要局限。情景预防理论为认知和防治集体腐败提供了新的理论视角和实践引导。因此,必须在集体腐败特征、类型及其成因分析的基础上,从削减外在引致环境、强化内在防控机制,切断内外关联三方面入手严惩集体腐败。     

儒学思想与当前幸福观的构建

市场化和现代化不断在全球渗透、扩张,工具理性主义、世俗功利主义和极端个人主义严重挤压着幸福生活的空间。我国幸福度的提升遭遇了很多隐忧,越来越陷于物化和利益私我化泥潭的民众,享受着几何级数递增的丰硕物质财富,幸福感却没有随之增加。挖掘和坚守儒学幸福思想,有利于构建一种超越物欲、物役、“GDP崇拜”的当前幸福观,形成和谐社会的价值共识理念。引领人们实现更美好、更尊严的可持续性幸福生活。     

Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards

The onus of proof in criminal cases is beyond any reasonable doubt, and the issue on the lack of complete internal validation data can be manipulated when it comes to justifying the validity and reliability of the X-chromosomal short tandem repeats analysis for court representation. Therefore, this research evaluated the efficiency of the optimized 60% reduced volumes for polymerase chain reaction (PCR) amplification using the Qiagen Investigator® Argus X-12 QS Kit, as well as the capillary electrophoresis (CE) sample preparation for blood samples on Flinder's Technology Associates (FTA) cards. Good-quality DNA profile (3000–12,000 RFU) from the purified blood sample on FTA card (1.2 mm) were obtained using the optimized PCR (10.0 μL of PCR reaction volume and 21 cycles) and CE (9.0 μL Hi-Di™ Formamide and 0.3 μL DNA Size Standard 550 [BTO] and 27 s injection time) conditions. The analytical and stochastic thresholds were 100 and 200 RFU, respectively. Hence, the internal validation data supported the use of the optimized 60% reduced PCR amplification reaction volume of the Qiagen Investigator® Argus X-12 QS Kit as well as the CE sample preparation for producing reliable DNA profiles that comply with the quality assurance standards for forensic DNA testing laboratories, while optimizing the analytical cost.     

领导干部经济责任审计成果在消防队伍管理中的运用

经济责任审计是对领导干部的政治素质、决策水平审查考核的重要内容。文章对在消防队伍管理是如何运用领导干部经济责任审计成果的问题进行了探讨。     

首都单位内部治安防控网络建设问题初探

当前首都单位内部治保工作面临的形势十分严峻,工作环境发生了许多新变化,加强单位内部治安防控网络建设十分必要。经过近两年的实践,首都单位内部防控网络建设已经取得了明显成效,今后还需继续坚持“预防为主、单位负责、突出重点、保障安全”的工作方针,把维护稳定放在各项工作首位,精确指导单位内部安全防范工作,规范内保民警的警务活动,发挥公安机关打击犯罪的职能,深入开展基层基础工作,全面推动争创“双无单位”活动的深入开展。     

论黑恶势力“内部渗透”的防治

近几年来,我国黑恶势力犯罪现象日益严重,黑恶势力不仅渗透经济领域,而且向基层政权组织进行“内部渗透”。黑恶势力进行“内部渗透”大都遵循这样一条主线:壮大经济实力——寻求“保护伞”——向党政司法机关直接渗透势力。黑恶势力“内部渗透”之所以能得逞归因于某些基层政权组织的软弱与涣散等因素。防治黑恶势力向我内部渗透须采取综合治理手段。     

Deliberation,Democratic Decision-Making and Internal Political Efficacy

Empirical research into the possible positive consequences of deliberation increasingly reveals that there is a complex relationship between deliberation and its effects on citizens. In this experimental study I examine the relationship between internal political efficacy and one type of deliberation: deliberative decision-making. I also test whether different structures of decision-making mediate between deliberation and internal political efficacy. The data suggest that deliberative decision-making had no direct effect on a global measure of internal political efficacy. Participants in face-to-face deliberative decision-making, though, had higher scores on a situation-specific measure of internal political efficacy than participants who only voted. The structures of decision-making had no effect on either measure of internal political efficacy. These results support claims that deliberation will not necessarily lead to direct, positive effects on citizens internal political efficacy, but they also highlight the likelihood that face-to-face deliberation can lead citizens to feel more competent in their deliberative abilities.