抗丁丙诺啡单克隆抗体的制备

目的建立抗丁丙诺啡单克隆抗体的杂交瘤细胞株，制备高特异性的丁丙诺啡单克隆抗体，并对其免疫学特性进行鉴定。方法在丁丙诺啡的分子上连接活性羧基基团，通过缩合反应将丁丙诺啡半抗原连接于血蓝蛋白（KLH）和小牛血清白蛋白（BSA），形成完全抗原。以完全抗原免疫Balb／c小鼠，通过细胞融合，筛选等杂交瘤技术，建立稳定的分泌抗丁丙诺啡单克隆抗体的杂交瘤细胞株。通过腹腔注射杂交瘤细胞，诱导小鼠产生含有单抗的腹水。用辛酸-硫酸铵加亲和层析法纯化抗丁丙诺啡单克隆抗体。采用酶联免疫反应和胶体金膜层析实验测定丁丙诺啡单抗的特异性以及免疫反应动力学参数。结果共获得3株分泌抗丁丙诺啡单克隆抗体的杂交瘤细胞株，分别命名为7E6，6G4和3C2。7E6，6G4抗体灵敏度为10．0ng／ml，3C2抗体灵敏度为20．0ng／ml。7E6，6CA和3C2抗体的亲和常数分别为3．6×10^-9 mol／L，4．3×10^-9 mol／L和6．3×10^-9 mol／L。特异性测试结果表明7E6和6G4抗体与40种药物、毒品无任何交叉反应，而3C2抗体与吗啡有交叉反应。结论杂交瘤细胞株7E6和6G4产生的抗丁丙诺啡单克隆抗体具有很高的特异性和灵敏度。     

论刑事诉讼中法官举证裁量权

抗辩式刑事庭审方式的中心环节是核查证据,法官举证裁量权是准确认定案件事实,公证作出判决及提高庭审效率的关键。借鉴国外经验完善我国的法官举证裁量权内容,是刑事诉讼立法完善的一项重要内容。     

论私文书证明力的推定规则

私文书形式证明力的有无可以从书证的名义人真实、形式真实两个层面来判断，名义人真实可以从名义人的签章真实来推定，形式真实可以通过名义入真实来推定。在确定实质证明力方面，司法推定具有一定作用。举证人对私文书的真实负证明责任，适用推定规则时，当事人对基础事实和推定事实的证明主题和证明标准均有所区别。     

科学与法律领域的真相

审判过程并非仅仅是为了"查明真相"。自从美国的法律系统开始使用科学证人以来,法律领域就面临着混乱的局面。自从Frye规则到Daubert标准再到Kumho Tire标准,乃至发展为修改后的《联邦证据规则》702条,尽管法律系统经历了上述诸多努力,人们仍然不会相信法律系统能够从科学信息中获得预期的收益。科学主张和理论或真或假,它们的真或假是一个客观的问题。法律裁决可以断定法律真理为真,也可以断定"所谓的科学真理"为真。只有科学命题所描述的自然界现象和事件的性质——而非有关证据可靠性的法律裁决,也非法庭上的论证和交叉询问——能够证明真的科学命题为真,证明假的科学命题为假。     

刑事诉讼中私人不法取得证据之证据能力研究

私人不法取证是私人以非法侵犯被取证人合法权利的方式取得证据的行为。私人不法取证本质上是一种侵权行为，但与公权机关非法取证存在诸多不同。关于私人不法取得的证据是否具有证据能力的不应排除说、法秩序一元说、权衡理论等诸家学说中，权衡理论更具有合理性。针对我国当前立法对私人不法取证问题规定的空白，应根据权衡理论对私人不法取证进行分类排除。     

Building informal knowledge‐sharing relationships between policy makers and academics: Insights from a PM&C engagement project

This article outlines an innovative project to encourage knowledge sharing and engagement between academics and a policy team within the Department of the Prime Minister and Cabinet (PM&C). The project was designed to enhance policy advice by drawing on a group of academics outside the policy ranks to act as a sounding board, to question, and provide differing perspectives within the policy process of crafting advice to government. External expertise and particularly academic research has the potential to improve evidence‐based policy but often fails to be specific or timely for those at the “pointy end” of policy decision making. An informal knowledge sharing framework has the potential to improve the exchange of information through confidential and targeted conversations between researchers and public servants as one way of overcoming these barriers.     

Mobilising evidence in public policy: The contribution of the evidence provider

This article contributes to the conceptualisation of evidence‐based policy through providing a framework of the key factors that influence research utilisation, including those that shape the quality of evidence provided. We apply this framework to an analysis of public policy to regulate the remuneration of freight truck drivers to improve workplace safety in their industry. Recent policymaking concerning the regulation of truck drivers’ remuneration in Australia provides an opportunity to examine the quality and utilisation of research evidence by external evidence providers in opposing political contexts. This article suggests the need for informed and vigilant scrutiny of the contributions of evidence providers to government policymaking, particularly in the case of wicked policy problems about which there are sustained ideological differences that underpin problematisation, research interpretation, and conceivable policy solutions.     

Opaque transparency: How material affordances shape intermediary work

How do the material aspects of intermediary work affect regulators, targets, and beneficiaries? To shed light on this question, we studied an information intermediary in the form of a website and the organizations who founded it. Specifically, we analyzed FracFocus, a self‐regulatory initiative with strong industry ties, charged with disclosing data pertaining to the chemicals used in oil and gas wells completed using hydraulic fracturing technology (fracking) in the United States and Canada. We found that between 2010 and mid‐2017, the vast majority of legislation in states and provinces where fracking actively occurred was updated to mandate or encourage disclosure via FracFocus, meaning that it had a considerable effect on the trajectory of official regulation on fracking disclosure. We also found that FracFocus disclosed important data but did so in a manner that limited accessibility and reduced the comprehensibility of environmental and public health risks to beneficiaries. Our analysis suggests that the public's experience of such a device is one of opaque transparency, in which the line between official and non‐official regulation is blurred. We traced these outcomes to the material affordances created by FracFocus.     

域外毒品鉴定错案成因分析与防范借鉴

近年来,域外毒品鉴定错案高发,深刻暴露出毒品鉴定管理的内源性问题,值得我国相关领域工作人员借鉴。本文首先概述了毒品鉴定的特点;其次,根据近年来美国、加拿大、英国等地出现的典型毒品鉴定错案,对相关成因做系统梳理,为提升毒品鉴定管理质量、降低毒品鉴定错案的负面影响提供参考依据。本文将鉴定错案分为"错误"和"失误"两大类,并提出了通过提升实验室内部管理、加强实验室外部监督以及落实鉴定意见的质证认证等环节,预防毒品鉴定错案的系统方法。对于防范毒物毒品错案的发生具有重要的参照意义。     

Near-infrared spectroscopy combined with chemometrics to classify cosmetic foundations from a crime scene

Cosmetic smears are a form of trace evidence that can link the crime scene, suspects, and victims. Foundation and lipstick are the most common sources of cosmetics that can easily smear, with most current research focused on the evidential analysis of lipsticks. This research aims to create a database of cosmetic foundations on different materials and to access the robustness of using Near-infrared with chemometrics as a non-destructive technique to identify unknown samples collected from a crime scene. Small amounts of six shades of three brands of foundations were smeared on clothing materials, which were then analysed with a combination of Near-infrared with chemometric analysis. Principle component analysis (PCA) was used to reduce data dimensionality and explore potential patterns in sample separation and Linear Discriminant Analysis (LDA) was utilised to assign unknown samples to one of the established classes. The selected techniques proved to be promising for database construction and as a preliminary method of analysis, with 93% of the spectra being correctly classified. Notably, darker foundation shades were less likely to be correctly classified (90% classified correctly) compared to lighter ones (96.7% classified correctly). This could not be improved with Standard Normal Variate (SNV) data pre-treatment or selecting specific NIR regions. This finding is of particular importance; according to the Crime Survey for England and Wales (year ending March 2020) police recorded sexual offences demonstrated that those in Mixed and Black or Black British ethnic groups were significantly more likely to be a victim of sexual assault compared to White, Asian or Other ethnic groups. It is, therefore, crucial to add a wide range of foundation shades, particularly of darker tones, to the future database.