结核分枝杆菌与牛分枝杆菌PCR快速鉴别检测方法的建立

根据结核分枝杆菌与牛分枝杆菌基因组的比对分析结果,针对结核分枝杆菌与牛分枝杆菌153bp、RD10和TbD1的3处差异缺失区域设计引物,分别建立了结核分枝杆菌与牛分枝杆菌PCR快速鉴别检测方法.应用建立的3种方法分别对84株结核分枝杆菌、3株牛分枝杆菌、51株非结核分枝杆菌及9种其他常见菌株进行了检测.结果显示,用针对153 bp缺失片段建立的PCR方法分别在结核分枝杆菌及牛分枝杆菌中扩增出645 bp及492 bp的目的条带;用针对RD10、TbD1建立的PCR方法分别在结核分枝杆菌及牛分枝杆菌中扩增出478 bp及361 bp的目的条带、358 bp及524 bp的目的条带.这3种PCR检测方法的准确率和特异性均为100%.敏感性试验结果显示,这些检测方法对结核分枝杆菌和牛分枝杆菌基因组DNA的检测极限均为10 pg.表明,此方法可用于牛源或人源结核分枝杆菌及牛分枝杆菌的快速鉴别检测.     

Meet the Narco: increased competition among criminal organisations and the explosion of violence in Mexico

Several previous studies have found that interventions by security forces against criminal organisations result in increased violence related to organised crime. However, much less is known about how and why this effect occurs. Our study not only identifies the causal mechanisms that explain this outcome, but also evaluates the empirical validity of these mechanisms. Employing a novel data set, we find that following security-force intervention, the number of criminal organisations increases, and such greater fragmentation in turn raises the incidence of violence among criminal organisations as the relative power of the organisations changes. We employ a mediation model to verify the existence of these causal mechanisms. In addition, we find a decreasing rate of rise in levels of violence as the number of organisations increases.     

中国法治秩序建构理论的反思与重构--以日常生活批判理论为视角

本文运用日常生活批判理论对当代中国法治秩序建构理论进行了反思,认为当前法治秩序建构理论的致命弱点在于主体维度的缺失;法治秩序建构的本质在于由传统的日常生活世界向非日常生活世界跃迁;当代日常生活作为传统法律文化的寓所对于法治秩序的建构形成巨大的阻滞与消解因素;日常生活批判对于法治秩序的建构具有根基性价值.进而提出重构当代法治秩序建构理论的设想,即建构一套以国家(政府)为动力,以社会为基础,以日常生活批判和深层文化启蒙为主体维度的国家·社会·日常生活批判三位一体的新的法治秩序建构理论.     

落实科学发展观推动柳州市经济社会跨越式发展

党的十六届四中全会提出,坚持以人为本、全面协调可持续的科学发展观,更好地推动经济社会和人的全面发展.深刻领会和准确地把握科学发展观的精神实质,自觉用以指导柳州市经济社会发展,对于加快柳州市改革开放和现代化建设步伐,顺利实现再造一个工业柳州市,在全区提前五年率先实现全面建设小康社会的奋斗目标具有重要意义.     

关于构建和谐劳动关系的哲学思考

构建和谐的劳动关系是转型时期中国建设和谐社会面临的重要课题。生产方式的进步是建立和谐劳动关系的决定因素;合理的分配制度是制约劳动关系的关键;健全的集体协商制度是实现和谐劳动关系的基本保障;实现和谐的劳动关系是建立和谐社会的重要内容。     

邓小平社会主义本质概括的哲学蕴意

邓小平的社会主义本质理论包含着对马克思关于科学社会主义理论的重大发展 ,并蕴涵着丰富的哲学思想。在“解放思想 ,实事求是”的科学世界观和方法论基础上 ,此一概括将生产力与生产关系、基础本质与价值本质辨证地统一起来 ,体现了彻底的唯物史观 ,深刻地揭示了社会主义本质是一个辨证发展过程的科学思想。     

6+1STR试剂盒和电泳凝胶EX-Q20用于DNA快速检验

目的 建立快速个体识别STR分型方法.方法 取采集于FTA上的血样200份,经打孔仪取等量血痕分别使用6+1 STR试剂盒结合新型毛细管电泳凝胶EX-Q20进行快速电泳分析和使用SinofilerTM试剂盒结合POP4胶进行电泳分析,比较两者所耗费时间和结果的差异. 结果 6+1 STR试剂盒结合新型毛细管电泳凝胶能够...     

探析农村黑恶势力形成的社会心理基础

农村黑恶势力的孳生、发展与其相应的社会心理基础有着必然的关系。其形成的社会心理基础,包括心理的互动性、集团内聚力、从众现象、社会心理需要与公共权力弱化等方面的内容。必须采取有效措施,充分发挥社会规范的综合治理作用,以减少或消除农村黑恶势力形成的社会心理基础。     

The mental health and substance misuse needs of male ex-armed forces personnel in prison

Ex-armed forces personnel constitute the largest known occupational group in prison but there is little evidence regarding their mental health, or substance misuse, needs. A total of 105 participants were interviewed and measures assessing symptoms of common mental health (CMH) problems and substance misuse were completed along with a review of their health care records. Forty (38%) participants screened for current CMH problems (CCMH) and high levels of dual symptomology and alcohol misuse were assessed. Thirty-nine (37%) had a mental health diagnosis recorded, most commonly for post-traumatic stress disorder (PTSD), depression and personality disorder. Those who screened for a CCMH problem were more likely to have pre-service vulnerability to negative health outcomes and those with dual symptomology were more likely to have experienced deployment during their service. Findings suggest the mental health needs of this group are similar to the general prison population. Potentially higher prevalences of PTSD and alcohol misuse may direct service provision.     

Dynamic Attenuation: Terrorism,Transnational Crime and the Role of the US Army Special Forces

This article analyses the ability of the US Army Special Forces to combat illicit networks (criminal and terrorist) through ‘dynamic attenuation’. It is argued that a process of dynamic attenuation, where network ties and not the actors in the network are targeted, should replace the current US strategy of ‘killing or capturing’ criminal agents threatening US interests. By dynamically attenuating (not destroying) the ties between and among criminal actors and criminal organisations, the US can effectively reduce the capability of criminal organisations to operate and achieve their missions (profit and/or terror). This argument is substantiated by assessing the environments where criminal networks thrive, the characteristics of criminal networks, the utility of targeting networks instead of individual actors, and through a comparison of criminal organisations' and US Army Special Forces' strengths and weaknesses. This article concludes with implications and recommendations for US policy in the fight against criminal organisations.