鸡myostatin基因成熟区段DNA的克隆及其在甲醇酵母中的融合表达

根据GenBank中鸡myostatin(AF019621)成熟区段的cDNA序列设计了1对引物,利用PCR技术扩增了萧山鸡myostatin的成熟区段,构建了重组真核表达载体myostatin-pPIC9K,并在甲醇酵母中融合表达了myostatin蛋白。结果发现,萧山鸡myostatin基因存在2处碱基变异:T204→C204(编码的氨基酸没有变),C205→T205(编码的氨基酸由Pro变成Ser),从而产生了1个EspⅠ或Bpu1102Ⅰ限制性内切酶酶切位点。核苷酸序列同源性分析表明,myostatin基因成熟区段DNA在不同物种间具有高度的保守性。SDS-PAGE鉴定结果表明,表达的目的蛋白(26 ku)具有生物学活性。     

破产重整制度中各方主体的角色定位

新《破产法》(草案)①引入了破产重整制度,并对其专设一章,可见重整制度之重要。破产重整的立法精神是再建主义精神。笔者认为,要想使破产重整制度发挥其最大功效,实现立法者拯救企业的立法本意,最关键的是在利益制衡原则的基础上,对破产重整制度各方主体的角色进行科学合理的定位,在此基础上,对破产重整制度中各主体的权限与职责做明确科学的规定,这样才能最大限度的发挥破产重整制度的作用。本文基于破产重整的制衡原则,对破产重整中各方主体进行了角色定位,希望能构建起破产重整制度中各方主体间完善科学的关系,从而加深对破产重整制度的理解,促进破产重整制在我国度发挥其最佳效果。     

DNA commission of the International Society of Forensic Genetics: Recommendations on the interpretation of mixtures

The DNA commission of the International Society of Forensic Genetics (ISFG) was convened at the 21st congress of the International Society for Forensic Genetics held between 13 and 17 September in the Azores, Portugal. The purpose of the group was to agree on guidelines to encourage best practice that can be universally applied to assist with mixture interpretation. In addition the commission was tasked to provide guidance on low copy number (LCN) reporting. Our discussions have highlighted a significant need for continuing education and research into this area. We have attempted to present a consensus from experts but to be practical we do not claim to have conveyed a clear vision in every respect in this difficult subject. For this reason, we propose to allow a period of time for feedback and reflection by the scientific community. Then the DNA commission will meet again to consider further recommendations.     

坚持和完善社会主义初级阶段的基本经济制度

坚持和完善社会主义初级阶段的基本经济制度 ,必须做到两个“毫不动摇” :一是毫不动摇地巩固和发展公有制经济 ;二是毫不动摇地鼓励、支持和引导非公有制经济发展     

目前我国物业管理市场存在的问题分析

目前我国物业管理市场虽然发展很快,但问题不少。这个市场本身尚不成熟,物业管理纠纷不断发生,制度缺失依然存在。了解矛盾才能解决矛盾,不断的完善和加强制度建设才能使物业管理市场不断的规范。     

现代企业制度下内部审计的作用

内部审计是强化企业内部财务控制、堵塞财务管理漏洞的一种企业内部约束机制,内部审计可提高企业管理水平和企业经济效益,内部审计可保护公共财产,实现国有资产的保值、增值,配合外部审计,完善我国审计监督体系。     

关于领导哲学研究的一些思考

领导哲学是近一段时间哲学界新型的话题,也特别引人注目,可以说仁者见仁,智者见智,笔者就领导哲学进行了界定,并提出了它的研究对象、理论体系和地位作用,以期给领导者的领导艺术提供思考。     

钢结构交错桁架的应用及抗火性能研究简述

钢结构交错桁架体系是一种经济、实用、高效的建筑结构体系,本文对交错桁架的结构特点及其在国内外的应用进行了介绍,对钢结构交错桁架体系在火灾作用下的力学特性进行了初步地探讨,提出了一些钢结构交错桁架体系抗火设计方面有用的建议。     

The evolution of European legal integration

This article contributes to the development of theories on European integration by testing and exploring statistical models on the long-term development of legislative activity of the European Commission. Drawing on legal information gained from the European Union’s PreLex database and analyzing it with the help of statistical analyses, we map out growth patterns of EU law between 1976 and 2003. We construct time-series models and models based on non-linear regression. While the performance of models based on the traditional theoretical approaches, intergovernmentalism and neo-functionalism, is rather poor, the analysis suggests that nonlinear dynamic models might be an interesting avenue for future conceptualizations of the EU integration process. This article is based on a paper presented at the ECPR Standing Group on the European Union Second Pan-European Conference on EU Politics, “Implications of a Wider Europe: Politics, Institutions and Diversity”, 24–26 June 2004, Bologna, Italy. We would like to thank the seminar participants for useful comments.     

美、日、欧现代物流发展的比较与启示

现代物流是系统管理的思想、理念和技术,集成系统化是现代物流发展的一个重要特征。美国、日本和欧洲现代物流发展各具特点,但对我国构建现代物流体系都具有启示作用。我国物流正处于由传统物流向现代物流转型的阶段,应当吸收西方发达国家现代物流发展的经验,建设与经济发展相适应、与人口资源环境相协调的现代物流网络体系,构建现代物流发展政策措施体系,加速物流现代化的我国现代物流的发展。