基于抗原蛋白Fiber-2及细胞因子佐剂的Ⅰ群4型禽腺病毒亚单位疫苗的开发

为了开发一种抗I群4型禽腺病毒(FAd V-4)的亚单位疫苗,选择I群禽腺病毒血清4型流行毒株衣壳蛋白Fiber-2作为保护性抗原蛋白。利用昆虫细胞-杆状病毒系统生产Fiber-2蛋白,通过Westernblot检测重组蛋白的表达情况,利用双向琼脂扩散法检测重组Fiber-2的免疫原性。为了增强亚单位疫苗的免疫保护效果,添加鸡源白细胞介素2(chIL-2)和鸡源γ-干扰素(ch IFN-γ)作为免疫佐剂,与重组Fiber-2单独或混合使用,制备多种形式的亚单位疫苗,并进行动物攻毒保护试验。重组蛋白的Western-blot鉴定结果显示,63 ku处出现Fiber-2蛋白目的条带;双向琼脂扩散试验结果显示,重组蛋白效价达到1∶128,表现出良好的反应原性。动物攻毒保护试验结果显示,单独使用Fiber-2免疫SPF鸡,在50 μg/只的剂量下,可为SPF鸡提供100%的保护效果,以chIL-2和chIFN-γ为免疫佐剂,与重组Fiber-2联合免疫SPF鸡,可显著增强Fiber-2在低剂量(10 μg/只)下的免疫保护效果,上述结果表明重组Fiber-2联合chIL-2和chIFN-γ细胞因子佐剂所制备的亚单位疫苗在抗I群4型禽腺病毒感染领域具有良好的应用前景。     

Confidence in an election authority and satisfaction with democracy: Evidence from a quasi-natural experiment of a failed election in Sweden

Contemporary research on electoral integrity has focused on the functioning, evaluation, and legitimacy of electoral processes in emerging democracies. By contrast, this study investigates whether a failed election in a well-established democracy can affect individuals' evaluations of the electoral management body, the Election Authority, and whether those evaluations affect satisfaction with democracy. Using the case of a Swedish regional election in 2010 that had to be rerun due to procedural mistakes in the vote handling, we found that, in the short term, individuals’ confidence in the Election Authority was reduced after it was announced that the election had to be rerun because of the mistakes. Subsequently, this decreased confidence was strongly associated with less satisfaction with democracy at the regional and national level. As good news for the authority, after a successful rerun election, confidence rebounded to the levels prior to the failed election.     

Fatal intoxication involving 2-methyl AP-237

Novel synthetic opioids contribute considerably to the opioid epidemic, especially with the frequent emergence of structurally similar compounds. This case report describes a fatal intoxication involving 2-methyl AP-237. A 54-year-old Caucasian male was found deceased from an apparent drug overdose. A plastic container labeled “2MAP” and a cut straw were found in the decedent's backpack at the scene. A white substance found in the container tested positive for fentanyl by field testing. According to his medical history, the decedent was treated for a drug overdose 3 years prior to his death. With no diagnostic findings at autopsy, the case was submitted for toxicological analysis. An unknown substance was detected in peripheral blood and urine using gas chromatography with nitrogen phosphorous detection (GC-NPD). Further testing was conducted using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-QTOF-MS) which confirmed the presence of 2-methyl AP-237 and potential metabolites in blood and urine. Quantitation by GC-NPD revealed concentrations of 2-methyl AP-237 in blood and urine at 480 ng/mL and 4200 ng/mL, respectively. The toxicological analysis also identified and quantitated alprazolam in the blood at 55 ng/mL. Additionally, the metabolism of 2-methyl AP-237 was investigated and three hydroxylated metabolites were identified in peripheral blood and urine. Limited literature is available for the detection and quantitation of 2-methyl AP-237 in postmortem specimens. Given the toxicological findings with unremarkable autopsy findings, this case is an example of a fatal intoxication involving 2-methyl AP-237.     

2,4,6-Tri(2′-pyridyl)-1,3,5-triazine for determination of iron(II), Iron(III), and total iron contents on human palms

When iron-based tools, such as knives or guns, are held, traces of iron can transfer to the skin. However, no previous studies have been published regarding the effect of the elapsed contact time on the transfer of iron species with different valences to the palm. Compared with 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine (PDT), 2,4,6-tri(2′-pyridyl)-1,3,5-triazine (TPTZ) was found to exhibit a higher sensitivity to iron(II) spectrophotometrically. This work employed 2,4,6-tri(2′-pyridyl)-1,3,5-triazine (TPTZ) and UV spectrophotometry to determine the amounts of iron(II), iron(III), and total iron transferred to human palms from iron tools. It was found that the palmar moisture level was an important factor in determining the amounts of total iron, including iron(II), transferred to the palm. For identical contact times, the amounts of total iron transferred to the palm was proportional to the palmar moisture, and the difference between the maximum and minimum amounts was 12 μg per hand. However, the amounts of iron(II) transferred to the palm gradually decreased over time for low palmar moisture levels, but steadily increased over time for high palmar moisture levels. Additionally, for average levels of palm moisture, the amounts of iron(II) and iron(III) transferred to the palm gradually decreased and increased, respectively, with longer contact times. Notably, this research could serve as a theoretical basis and guide for the detection of trace iron species with different valences on human palms for criminal investigations.     

The Responsibility to Protect at Ten: Glass Half Empty or Half Full?

Abstract

Far from having faded away, ten years after its formal adoption, the responsibility to protect (R2P), is arguably more relevant than ever. In the current overall context of protection crises, heightened in severity by the emergence of violent extremists, R2P has changed the way in which the international community characterises situations that involve protection failures, and has raised expectations about what should occur when atrocity crimes have been committed or are imminent. UN member states now agree that prevention is at the core of R2P, that international action should employ the full range of diplomatic, political and humanitarian measures, and that military force should only be considered as a measure of last resort. While there is continued contestation about particular aspects of R2P – as there is over much older normative advancements, such as human rights – R2P has helped to forge political consensus and build new institutional capacity to prevent and respond to atrocity crimes.     

Responsibility to Protect: Russia’s Approaches

Abstract

Russia’s predominantly suspicious and even negative attitudes toward R2P are closely related to its traditional attachment to the notion of sovereignty, but its reluctance to ‘bless’ the use of force with R2P also serves as a pretext to cover various instrumental goals. Russia’s more assertive foreign policy has exacerbated this trend. Disagreements stem from differences between Russia and the West both in their conceptual approaches to security and in their assessments of specific cases. In particular, Russia has an existential concern over possible application of R2P by extra-regional actors in its immediate post-Soviet vicinity. However, in the conflicts around South Ossetia (2008) and Crimea / Southeastern Ukraine (2014-), there was a noticeable trend to refocus R2P-related arguments in support of Russia’s own actions. By and large, R2P continues to be perceived as a Western attempt to establish certain rules of behaviour which require caution and prudence. Nevertheless, more positive attitudes do not seem impossible. To play a prominent role in the evolving international system, Russia will have to make the R2P segment of its foreign policy more salient and overcome the lag in promoting this concept as a working tool indispensable for cooperative and responsible leadership.     

The discursive construction of intervention: selves,democratic legacies,and Responsibility to Protect in French discourse on Libya

This article analyses French executives' and lawmakers' legitimisations of the intervention in Libya with the aim of understanding the discursive construction of intervention. It investigates the arguments in favour of intervention and the oppositions they were confronted with. To these arguments belong a re-evaluated democratic legacy of France, an identification with the Libyan people, and a debate on Responsibility to Protect and the rule of law in world politics, which have a broader relevance for French actorness abroad. The article applies the Essex School discourse theory and techniques from Interpretive Policy Analysis on executive speeches and parliamentary documents for structuring the debate and for estimating the strength of ideas in their interdiscursive configuration. An ideal-typical explanation of the legitimisation of intervention and of the choice of one policy over another is made. The article argues that going to war in Libya equated to a question of cultural appropriateness.     

In Defense of the Responsibility to Protect: A Response to Weissman

This article defends the Responsibility to Protect (R2P) doctrine (adopted by the United Nations in 2005) against critiques by Fabrice Weissman in this journal, and against similar criticisms of humanitarian intervention and human rights norms made by postmodern thinkers in the Nietzschean tradition, such as Alain Badiou and Anne Orford. I argue against Weissman that R2P can be effective in stopping or preventing mass atrocities, and in particular that opposition to military intervention in Syria during the 2013 debates was a terrible mistake. Moreover, the moral ground for humanitarian aid efforts is the same as the basis for forceful rescue from mass slaughter, ethnic cleansing, and persecution (when other conditions of just war can be met). Weissman's critiques misinterpret just war theory on key points and rely on inflated rhetorical strategies inspired by extreme forms of cultural and moral relativism that are intellectually bankrupt—both in blaming “Western imperialism” for most crimes against humanity committed by tyrants, and in leaving hundreds of thousands without the only protection that could prevent their murder and exile. These extreme positions and the strained rhetorical devices used to defend them do not deserve the wide respect they command in some parts of academia.     

网络考试系统分析

组织学生考试是高校重要的工作,但现在的考试形式大多是教师手工出题、阅卷,虽然出现了相应的答题卡形式,减少了老师一定的阅卷负担,但效率还是较低的。利用网络和数据库技术,结合目前硬件价格普遍下跌与宽带网大力建设的有利时机,基于B／S模式研究开发了试题库与在线考试系统。它运用方便、操作简单,效率较高,同时也大大减轻了教师出题、组卷和改卷等繁重的工作量。     

"2+1"教学培养模式的构建思考

公安高等教育是为各级公安机关输送合格人才的主渠道,而公安职业教育主要面向市县基层公安机关,培养公安一线应用性专门人才。公安职业教育人才培养模式的问题是新世纪公安教育迫切需要解决的问题,改革的基本思路应是对人才培养目标进行恰当的定位,实行公安职业教育,为公安机关培养一线高素质应用性人才。对此,公安职业教育培养模式必须重新构建准确定位,突出公安教育的职业性特色,课程设置和教学内容要突出应用性和实践性,实行"2 1"教学培养模式是必要的。