口蹄疫病毒O型和A型交互反应性单克隆抗体的筛选与鉴定

不同血清型口蹄疫病毒(FMDV)之间不能产生交叉免疫保护,但是血清学的交叉反应确实存在。本研究旨在建立一种筛选型间交互反应性抗体的方法,以期为解析FMDV血清型间保守的抗原结构奠定基础。本方法主要依据单个B细胞抗体技术,首先采用两种不同的荧光染料FluoProbes 647H和Pacific Blue分别标记O型与A型FMDV 146S灭活抗原,然后利用流式细胞分选技术,从免疫牛外周血单个核细胞(PBMCs)中分选出结合两种抗原的特异性B细胞,通过单个B细胞抗体基因测定,获得Ig G抗体重链与轻链可变区编码序列,并将可变区基因插入含有牛IgG抗体恒定区的pcDNA3.4载体中,构建IgG抗体的重链与轻链表达质粒。将重链与轻链表达质粒共转染中国仓鼠卵巢悬浮细胞(CHO-S)进行完整抗体表达。结果显示,通过该方法获得了5株FMDV特异性单克隆抗体,其中4株(H64、R82、I16、R29)经间接免疫荧光(IFA)检测都可特异性识别O和A型FMDV;ELISA结果显示,H64、R82、I16与O、A型抗原均具有较好的亲合力;Western-blot结果显示,I16可特异性结合O、A型FMDV结构蛋白VP2中的线性表位,说明在衣壳蛋白VP2上存在血清型间保守的抗原表位,通过本研究进一步加深了对FMDV抗原结构的认识。     

Political accountability,fiscal conditions and local government performance—cross‐sectional evidence from Indonesia

Why are some local governments more successful than others in managing resources and delivering services? And even more vitally, how can malfunctioning governments be reformed so that they perform their responsibilities more effectively? This article contributes to our understanding of theses overarching questions by exploring the interactions between political institutions and public sector performance in the context of decentralisation and local governance. It shows–both theoretically and empirically–that performance outcomes are determined by the extent to which people can hold their governments accountable through political institutions. The basic hypothesis underlying this research is that political accountability, either by encouraging sanctions upon non‐compliant public agents or simply by reducing the informational gap regarding government activities, will create forceful incentives for elected officials and civil servants to reduce opportunistic behaviour and improve performance. Using a cross‐sectional regression the hypothesis is empirically tested against evidence from newly empowered local governments in Indonesia. The empirical findings broadly support our hypotheses. Improved public services on the ground, both in terms of quantity and quality, require informed and well functioning decision‐making processes that allocate resources to priority areas that meet the demand of the broader community. Copyright © 2008 John Wiley & Sons, Ltd.     

4株猪瘟流行野毒株病毒结构蛋白E2基因的核苷酸序列分析

应用RT-PCR和nPCR扩增由4株甘肃省近期(1997-1998)猪瘟流行野毒株的E2基因,将其克隆到pGEMTEasy载体上,经转化、筛选、鉴定后,测出核苷核序列.4株流行毒株的E2基因核苷酸序列同源性为89.2%-99.7%,相应的氨基酸序列同源性为93.8%-99.0%.这4株流行毒株;与C-株(疫苗种毒)E2基因的核苷酸序列同源性为82.2%-84.3%,相应的氨基酸序列同源性为87.9%-90.2%,表明近期猪瘟流行毒株与C-株的gp55蛋白之间存在一定的差异.     

论无单放货的法律问题

提单是海上货物运输的重要单证.随国际贸易和航运事业的不断发展,提单也从一般托运收据发展成为物权凭证.提单作为代表货物所有权的凭证,可以通过背书转让或抵押,它已具备了一种有价证券的性质.提单的内容、条款以及调整提单的法律也随现代国际贸易的迅速发展而日趋详细和完善.但是,近些年由于航运的变化,无正本提单提货的现象频繁发生,这种行为的产生对传统的航运制度带来的冲击和影响很大.无单放货问题在司法实践和航运实践都引起重视,对这一现象进行研究.     

Sample Mining and Data Mining: Combined Real-Time and Retrospective Approaches for the Identification of Emerging Novel Psychoactive Substances

Novel psychoactive substances (NPS) are synthetic drugs that pose serious public health and safety concerns. A multitude of NPS have been identified in the United States, often implicated in forensic investigations. The most common and effective manner for identifying NPS is by use of mass spectrometry and the true utility lies within nontargeted acquisition techniques. During this study, a liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) assay was developed, validated, and implemented for forensic toxicology testing. A SCIEX TripleTOF™ 5600 + with SWATH^® acquisition was used. Resulting data were compared against an extensive library database containing more than 800 compounds. The LC-QTOF-MS assay was applied to the reanalysis of biological sample extracts to discover emergent NPS. More than 3,000 sample extracts were analyzed, and more than 20 emerging NPS were detected for the first time. Among these were isopropyl-U-47700, 3,4-methylenedioxy-U-47700, fluorofuranylfentanyl, N-methyl norfentanyl, 2F-deschloroketamine, 3,4-methylenedioxy-alpha-PHP, eutylone, and N-ethyl hexedrone.     

Making a Place for Alternative Technologies: The Case of Agricultural Bio‐Inputs in Argentina

This article characterizes the ways in which the actors in charge of designing and implementing public policies intervene to promote the emergence of alternatives to problematic technologies. It is based on a case study conducted in Argentina that focuses on initiatives to promote the development of biological agricultural inputs in the context of increasingly controversial chemical inputs. The study spotlights the political, institutional, and semantic efforts made by policy makers and public administrations to ensure these new inputs find their way into organizations and onto their agendas. Their work consists in attenuating the boundaries between chemical and biological inputs, and reducing opposition by creating categories and organizations that downplay potential dissension and highlight the possible coexistence of technological paradigms. Contrary to what the injunctions of technological substitution suggest, we show that putting alternative technologies on the public agenda depends largely on their inclusion in institutional and regulatory infrastructures originally designed for technologies that are likely to decline. More broadly, it relies on the construction of continuity between the two types of technologies.     

Determinants of Firm‐Level Lobbying and Government Responsiveness in Agricultural Biotechnology in China

Understanding what stimulates agribusiness firms to lobby the government and what makes the government responsive to lobbying are the two issues that have been discussed extensively in the debates concerning determinants of biotechnology policy. This paper examines the factors influencing agribusiness firms' lobbying and government response using econometric modeling on a new data set of 160 leading agribusiness firms in the food, feed, chemical, and seed industries in China. The results show that approximately 10% of agribusiness firms lobbied the government about biotechnology policy and regulations and over half of those that lobbied received a verbal or written acknowledgment from government agencies. Seed and feed companies are more likely to engage in lobbying than chemical companies. Owning GM patents not only has a positive impact on firms' lobbying activities, but firms with these patents are more likely to receive a government response to their lobbying efforts. The experience of selling GM products does not significantly influence lobbying activities or response from the government.     

Applying Stone in a Western Landscape: Ranchers,Conservationists, and Causal Stories in the “American Serengeti”

As the emergence of nongovernmental conservation efforts generates conflict among various stakeholders, the causal story that each party articulates regarding conservation and the causes of land degradation reflects their unique interests. This study uses existing literature to evaluate causal stories surrounding a contemporary conservation effort: Montana's American Prairie Reserve. Through qualitative review of web‐based documents and newspaper articles, it generates a preliminary account of key stakeholders' causal stories. The case study suggests that parties who might be disadvantaged by ascribing responsibility for environmental harms in an adversarial fashion may instead elect to articulate causal stories that are more neutral than existing approaches might forecast. The study concludes by suggesting that further development of causal story literature may enable it to better address contemporary conservation efforts.     

Divergent fates: company unions and employee involvement committees under the railway labor and national labor relations acts

Well known is that the National Labor Relations Act (NLRA, 1935) in the United States places a largely per se ban on nonunion employee representation (ER) groups which deal with employers over a term or condition of employment. Much less well known is that America’s other labor law, the Railway Labor Act (RLA, 1926), takes a different approach and permits employers to operate such councils and committees as long as they do not perform a collective bargaining function or interfere with workers’ free choice of a bargaining agent. Thus, under the RLA Delta Air Lines is able to operate what is today the closest living approximation to a 1920s-style ER plan while hundreds of other companies (e.g. Polaroid) under the jurisdiction of the NLRA have been forced over the years to disband similar groups on grounds they are a proscribed company union. No study to date has explored the history behind the RLA and NLRA’s divergent treatment of nonunion ER groups so this article takes a first look. The main part of the story covers the 1920–1935 period and examines the events, people, and experiences associated with company unions and ER in, respectively, the rail and manufacturing industries and why the legislative outcome in the former was a permissive stance on nonunion committees but prohibitive in the latter. The last part of the paper fast-forwards the RLA-NLRA story from the 1930s to contemporary law and practice in order to demonstrate how “history matters” when it comes to what employers can and cannot do with nonunion representation groups, such as works councils, participation and involvement committees, and dispute resolution forums.     

平台异化还是因债致罪？——P2P网贷涉非法吸收公众存款罪裁判文本实证研究

文章采用文献研究的方法对83份P2P网贷平台构成非法吸收公众存款罪的裁判文书进行实证分析。通过对平台异化模式的定性分析和平台负债情况的定量分析以及与刑事责任程度的交叉分析,文章认为,平台异化是P2P网贷平台构成非法吸收公众存款罪的基础性因素,负债规模、存款人人数和无法清偿的数额等三个因素都对刑事责任的程度产生影响。而且相比之下,无法清偿的数额对刑事责任影响的程度最为显著。实际上对P2P网贷平台追究非法吸收公众存款罪的刑事责任是因为网贷平台无法清偿举债数额巨大,即因债致罪。这种因债致罪的司法行为逻辑,可以从司法者作为“清场者”的功能定位和对社会秩序的价值追求来解释其合理性,但是从多元价值权衡和刑事民事分离的角度分析,其又是不合理的。