DNA指纹图在法医学鉴定中应用的研究(Ⅰ)

应用 MYO DNA 探针对中国人进行了 DNA 遗传指纹图检验。从两个家系16人及100个无关个体中取肘静脉血提取 DNA,用限制性内切酶 HinfⅠ或 HaeⅢ水解,1%琼脂糖凝胶电泳分离,经 Southern印迹转移,MYO DNA 探针杂交,获得了清晰可辨的 DNA 指纹图谱。结果每个个体在3.0Kb 以上均能检出10条以上杂交区带,个体间的相关概率<4×10~(-9),由杂交区带构成的图谱是个体特异的,杂交区带遵循孟德尔的显性遗传方式由亲代向子代遗传;具有 DNA Fingerprints 的特点。对两起亲子鉴定的案例进行指纹图检验,孩子所存在的杂交区带,除来自母亲外,其余可在嫌疑父亲带中找到,肯定了孩子与嫌疑人的父子关系。MYO DNA 探针在亲子鉴定与个人识别的法医学鉴定中有着重要的实用价值。     

EXCEL多元回归分析在痕迹数据处理上的应用

在痕迹检验技术中,很多推算公式是由回归分析预测法得出的。多元回归分析预测法的计算,虽然可以用常用计算器进行统计,但是数据复杂繁琐,工作量较大。Excel是一种办公系统软件,运用它的强大数据分析功能,只输入一次原始数据,而后全部以鼠标操作即可自动生成回归方程。文章以一组足迹数据分析为例,首先介绍相关分析,然后建立回归模型,最后计算出相关的回归方程。     

试析我国统一司法考试制度

2002年我国将实行统一司法考试制度。国家统一司法考试制度是指国家通过立法的方式确立的司法考试资格、司法考试科目和内容、司法考试方式和组织机构及管理的一项国家司法制度。为了实现考试面前人人平等,建立一项完备的统一司法考试制度是十分必要的。     

外交哲学的四个基本变量及实证分析

外交哲学的四个基本变量是实力、利益、观念与行为,其中实力、利益与现念都是自变量,行为是因变量。自变量决定因变量,实力、利益与观念分别作用并共同决定行为。同时,三个自变量之间还存在着相互作用的互动关系。     

PHI测查剖析在变态人格犯罪研究中的应用

随着科学技术的飞速发展 ,人们生活工作节奏相应加快 ,激烈的社会竞争使人们的心理压力日益加重 ,长期精神压力容易导致身心疾病、人格变态。了解变态心理特征 ,采用客观可靠的测量手段甄别心理变态者 ,对进一步研究变态犯罪具有理论指导意义 ,对司法实践活动也有所助益 ,可以丰富和发展我国变态犯罪心理的理论和实践 ,促进整个心理科学的发展     

A screening method for urinary methamphetamine--latex agglutination inhibition reaction test

Methamphetamine in urine samples from abusers was detected by the latex agglutination inhibition reaction test with latex-antibody (Latex-Ab) and latex-methamphetamine (Latex-MA) reagents. Anti-methamphetamine antibody was produced in rabbits by immunization with bovine serum albumin (BSA)-methamphetamine conjugate. Latex particles were coated with antibodies or with rabbit serum albumin (RSA)-methamphetamine conjugate to obtain Latex-Ab and Latex-MA reagents, respectively. The results are read at 4-5 min after mixing the latex reagents. The sensitivity of this method for methamphetamine was 0.4 micrograms/ml urine. Methamphetamine analogs (methylephedrine, amphetamine, phentermine, methoxyphenamine, ephedrine, beta-phenylethylamine, OH-methamphetamine, OH-amphetamine, and OH-ephedrine) all cross-react in varying degrees, while glucosiurea and albuminurea give false positive results in the tests. Though attention must be paid to these effects this simple and rapid test is suitable for the mass screening of urine samples.     

心理测试中的犯罪者心理研究

从犯罪者个性因素和犯罪环境因素两方面,对心理测试中的犯罪者心理进行分析。通过测前准备阶段分析犯罪者心理,缩小犯罪嫌疑人,确定侦查方向。正式测试时把握犯罪者心理,完整地将被测人与犯罪者区分开来,帮助侦查人员判断和逮捕犯罪嫌疑人,破获案件。     

甲基苯丙胺稀土荧光试纸条的研制

目的建立一种检测尿液中甲基苯丙胺物质的高灵敏度检测方法。方法以稀土荧光纳米颗粒为标记物,采用反应杯+试纸条的反应模式,建立了免疫层析法,用于尿液中甲基苯丙胺的定性检测。结果用所制备的试纸条检测尿液中的甲基苯丙胺,肉眼和仪器判读的敏性均为100ppb,与吗啡、亚甲二氧基苯丙胺、氯胺酮、苯丙酮、可卡因等无交叉反应;将制备的反应杯和试纸条置于37℃7d,试纸条的性能没有明显降低。结论本研究建立的甲基苯丙胺免疫层析法用于定性检测尿液中的甲基苯丙胺,具有较高的灵敏性和较高的应用价值。     

Inferring ancestral origin using a single multiplex assay of ancestry-informative marker SNPs

Tests that infer the ancestral origin of a DNA sample have considerable potential in the development of forensic tools that can help to guide crime investigation. We have developed a single-tube 34-plex SNP assay for the assignment of ancestral origin by choosing ancestry-informative markers (AIMs) exhibiting highly contrasting allele frequency distributions between the three major population-groups. To predict ancestral origin from the profiles obtained, a classification algorithm was developed based on maximum likelihood. Sampling of two populations each from African, European and East Asian groups provided training sets for the algorithm and this was tested using the CEPH Human Genome Diversity Panel. We detected negligible theoretical and practical error for assignments to one of the three groups analyzed with consistently high classification probabilities, even when using reduced subsets of SNPs. This study shows that by choosing SNPs exhibiting marked allele frequency differences between population-groups a practical forensic test for assigning the most likely ancestry can be achieved from a single multiplexed assay.     

Clinically Significant Trauma Symptoms and Behavioral Problems in a Community-based Sample of Children Exposed to Domestic Violence

This study assessed the associations of characteristics of domestic violence incidents with clinically significant levels of traumatic symptoms and behavioral problems in a socio-economically and ethnically mixed sample of 687 children participating in a community-service program for children witnessing violence. Study predictors included child/family demographic characteristics, type and chronicity of exposure, and child’s perceptions of control over the event and threat to personal safety. Outcomes consisted of traumatic symptoms and behavior problems. Results showed that perceived threat and control were associated with greater odds of clinically significant levels of several trauma symptoms (and behavior problems in the case of perceived threat) after adjusting for effects of demographic factors and violence characteristics. Child co-victimization increased odds of reaching clinically significant levels of traumatic symptoms compared to children who witnessed the event but were not victimized. Female sex and White ethnicity increased odds of specific trauma symptoms and behavior problems. Increasing age reduced odds of some trauma symptoms. Associations between predictors and one outcome measure did not generalize across the other outcome measure. Implications of study findings, and directions for future research are discussed.