乌头碱对培养新生大鼠心室肌细胞Connexin43蛋白磷酸化的影响

目的建立细胞水平的染毒模型,观察乌头碱对心肌细胞缝隙连接蛋白Connexin43(Cx43)的影响。方法实验分0.25、0.5、0.75、1.0、1.5和2.0μmol/L等6个不同浓度乌头碱染毒组,运用蛋白印迹法和免疫荧光技术,检测各组及对照组心肌细胞Cx43蛋白磷酸化状态的改变。结果蛋白印迹检测显示,不同浓度染毒组心肌细胞中Cx43蛋白总量与正常对照组无显著差异;0.5μmol/L乌头碱染毒后,Cx43开始出现脱磷酸化;当染毒浓度达到1.0μmol/L后,Cx43脱磷酸化显著,且1.5及2.0μmol/L染毒效果和1.0μmol/L染毒组相当。免疫荧光分析结果提示乌头碱作用后,心肌细胞Cx43蛋白在羧基端第368位点丝氨酸残基(Ser368)发生明显的脱磷酸化。结论一定浓度的乌头碱能诱导心肌细胞缝隙连接蛋白Cx43发生显著脱磷酸化。     

Structure of the nanoscience and nanotechnology applications literature

The Applications literature associated with nanoscience and nanotechnology research was examined. About 65,000 nanotechnology records for 2005 were retrieved from the Science Citation Index/Social Science Citation Index (SCI/SSCI) (SCI (2006). Certain data included herein are derived from the Science Citation Index/Social Science Citation Index prepared by the Thomson Scientific^®, Inc. (Thomson^®), Philadelphia, Pennsylvania, USA) Through visual inspection of the Abstract phrases, all the diverse non-medical Applications were identified, and the relationships among the Applications, both direct and indirect, were obtained. The medical applications were identified through a fuzzy clustering process. Metrics associated with research literatures for specific Applications/Applications groups were generated.     

中国对联合国的外交政策:1949-1971--以《人民日报》涉及联合国内容的文章为分析文本

通过对1949-1971年《人民日报》发表的2314篇有关联合国内容的文章进行文本分析,作者发现,“不满、谴责、批评”是这一时期中国对联合国外交的主要政策趋向。其中一个有趣的现象是,在朝鲜战争期间,中国虽然与联合国兵戎相见,但中国政府对联合国的批评是克制的、有限度的,甚至低于战前水平,此后,中国政府长期坚持对联合国的低限度批评政策。直到1959年,中国对联合国的批评度才迅速攀升,并一直保持到1969年,在其间的1966年、1967年,中国对联合国的批评度高达95.12%和92.45%。1971年中国政府对联合国的批评度下降到3.13%,达到23年的最低点。批评度的骤然下降表明,中国政府改变对联合国外交政策的进程是突变式的,而不是渐进性的,而突变动因就是1971年中华人民共和国政府恢复了在联合国的合法席位。     

慢性阿片依赖者在戒断阶段细胞免疫功能的研究

为科学地鉴定、预防毒品相关死亡提供一定的实验证据，采用免疫组化、细胞培养、核素掺入、ELISA等方法，研究26例用药模式相近的慢性阿片依赖者在戒断阶段的细胞免疫功能，并与健康对照组进行统计比较。结果表明：慢性阿片依赖者戒断期CD＋4细胞数目减少（30．40±9．45％，P＜0．05），CD＋4／CD＋8比例降低（1．27±0．19，P＜0．01）；PBMC合成DNA的能力下降（SI＝8．62±3．20，P＜0．01），产生IL－2的水平降低（23．78±8．77，P＜0．06）和产生IL－6的能力呈降低趋势（468．81±196．40，P＞0．05）；血清中免疫抑制性因子sIL－2R水平显著升高（566．33±265．50，P＜0．01）。慢性阿片依赖者在戒断阶段有多种免疫参数紊乱，细胞免疫功能低下。     

大学生手机依赖症的心理原因及防治对策

近年来出现了一种由于对手机过分依赖而形成的现代心理疾病——手机依赖症,给大学生的身心健康和学习生活带来了很大危害。手机依赖症的形成原因错综复杂,其中内因是大学生的心理因素,主要包括从众心理、心理诉求、特殊的人格特质和不稳定的情绪四个方面。因此,学校、教师、学生自身三方面要形成合力,使大学生克服手机依赖心理,养成良好的意志品质,从而实现身心健康发展。     

Forensic analysis of Telegram Messenger for Windows Phone

This article presents a forensic analysis methodology for obtaining the digital evidence generated by one of today's many instant messaging applications, namely “Telegram Messenger” for “Windows Phone”, paying particular attention to the digital forensic artifacts produced. The paper provides an overview of this forensic analysis, while focusing particularly on how the information is structured and the user, chat and conversation data generated by the application are organised, with the goal of extracting related data from the information. The application has several other features (e.g. games, bots, stickers) besides those of an instant messaging application (e.g. messages, images, videos, files). It is therefore necessary to decode and interpret the information, which may relate to criminal offences, and establish the relation of different types of user, chat and conversation.     

"易注"与中国传统哲学中的注释

注释是中国传统哲学中的一种主要方法,人们一直以“六经注我,我注六经”来概括和指认这种注释,然而,这是完全错误的,因为“六经注我,我注六经”是陆九渊的一个心学命题,根本就不是一种注释的原则和方法。对“易注”样本的分析以及相关方面的考察,重新理解中国传统哲学中的注释现象,可得出如下结论:中国传统哲学中的注释,其实质乃是用母本的结构语义来表达注释者的思想;这种注释不是寻求原义的语义训诂,而是中国古代哲学家的一种哲学写作方式。     

论手机短信犯罪的惩治与预防

短信犯罪是指以向不特定或多数手机用户发送短信为犯罪手段的严重危害社会的行为。短信犯罪因为发送的内容不同可以触犯较多的罪名,在司法实践中应依相关罪名定罪处罚;同时相关单位应加大预防短信犯罪的立法和监管力度。     

Recovery of single source DNA profiles from mixtures by direct single cell subsampling and simplified micromanipulation

Deconvolution of forensic DNA mixtures into their individual component DNA (geno)types is of great investigative value, though often complex and difficult. Two-person mixtures comprising a major and minor contributor are often easily interpreted although, when the DNA ratio of the two individuals is approximately equal (~1:1), deconvolution and interpretation becomes much more difficult. To address this issue, a physical separation of individual-, two- or three- cell subsamples prior to autosomal STR analysis was performed using a simplified micromanipulation technique paired with a decreased reaction volume and increased cycle number PCR. Using this method, single and multiple buccal epithelial cells were collected from a 1:1 two-person mixture (i.e. from individual 'A' and 'B') and directly amplified, omitting standard DNA extraction and purification steps. Single cell subsamples resulted in partial single-source profiles for both contributors while, in accordance with expectations of a quasi-binomial sampling schema, two- and three-cell subsamples resulted in single source informative partial profiles of individual A and individual B as well as complete consensus profiles, and equally mixed 1:1 (2-cell subsamples) and 2:1 (3-cell subsamples) admixed profiles of individual A and B. This proof-of-concept approach shows promise in permitting the DNA deconvolution of mixed samples where the individual contributors are present in similar amounts that would otherwise be difficult to interpret, resulting in an increase in evidentiary value. The subsampling approach can be readily investigated for DNA casework applications without additional investment in costly, new equipment, requiring only a stereo microscope and a tungsten needle.     

新藤黄酸对人脐静脉内皮细胞凋亡的影响

目的 探讨新藤黄酸对人脐静脉内皮细胞（human umbilical vein endothelial cells,HUVECs）增殖和凋亡的影响。方法 用不同浓度的新藤黄酸培养细胞24、48 h后,采用MTT法观察新藤黄酸对细胞生长的抑制作用;倒置显微镜下观察HUVECs细胞形态变化;通过DAPI染色后倒置荧光显微镜观察新藤黄酸对细胞形态学的影响;通过Annexin V-FITC/PI双染及流式细胞术检测新藤黄酸对HUVECs细胞凋亡率的影响;Western blot检测新藤黄酸对HUVECs中凋亡相关蛋白Caspase-3的表达变化。结果 MTT检测结果显示新藤黄酸能够明显抑制HUVECs的增殖,并呈时效及量效关系;荧光显微镜观察结果显示,新藤黄酸作用HUVECs后出现大量凋亡细胞;流式细胞术结果显示,与空白对照组相比,随着新藤黄酸浓度的增加,细胞凋亡率明显增加;Western blot检测结果表明Caspase-3蛋白表达水平显著增加。结论 新藤黄酸对HUVECs有明显的抑制作用,其作用机制可能是通过诱导细胞凋亡实现的。