UPLC-MS/MS法检测佐匹克隆及其在大鼠体内动态分布研究

目的建立安眠镇静药佐匹克隆的检测方法及其在大鼠体内动态分布模型。方法实验组大鼠用佐匹克隆橄榄油溶液(47.25mg/kg)灌胃给药,空白对照组大鼠采用橄榄油灌胃,分别于0.5、1、1.5、2.5、5、8、12h后采集心血后处死大鼠,分别取心、肝、肺、脾、肾、胃、大脑组织,采用超高效液相色谱-串联质谱法(UPLC-MS/MS)检测各组织中佐匹克隆的质量浓度。结果佐匹克隆与内标物SKF525A出峰时间分别为1.43、1.6min。各组织中佐匹克隆在5~5000ng/mL(g)线性关系良好。佐匹克隆在10、100、1000ng/mL三个浓度下日间、日内精密度良好,在各组织中平均萃取回收率高。灌胃给药后大鼠各组织中佐匹克隆含量在0.5~1h内呈上升趋势,在1h时达到峰值,在各时间点,佐匹克隆在胃壁组织中含量较其他组织高,心血和大脑组织中相对较少。结论本课题建立的UPLCMS/MS法动态检测大鼠各组织中佐匹克隆的含量具有高效性、可靠性的特点,这对今后法医学案件中涉及到佐匹克隆定性定量检验有一定的参考价值。     

政府信息公开“申请内容不明确”时“补正”的法律思考

在依申请的政府信息公开中,明确的申请内容是政府信息公开工作启动的前提之一。行政机关不能简单的以申请不符合法定形式为由拒绝申请人的申请,因为我国现行法律赋予了申请人对申请内容补正的机会。在司法实践中,有关申请内容是否明确、申请人补正是否规范、行政机关对补正后的申请如何答复等问题,由于立法缺乏具体规定、司法经验相对匮乏等原因,尚需审判庭深入思考和审慎处理。     

Social media and political communication in the 2014 elections to the European Parliament

Social media play an increasingly important part in the communication strategies of political campaigns by reflecting information about the policy preferences and opinions of political actors and their public followers. In addition, the content of the messages provides rich information about the political issues and the framing of those issues during elections, such as whether contested issues concern Europe or rather extend pre-existing national debates. In this study, we survey the European landscape of social media using tweets originating from and referring to political actors during the 2014 European Parliament election campaign. We describe the language and national distribution of the messages, the relative volume of different types of communications, and the factors that determine the adoption and use of social media by the candidates. We also analyze the dynamics of the volume and content of the communications over the duration of the campaign with reference to both the EU integration dimension of the debate and the prominence of the most visible list-leading candidates. Our findings indicate that the lead candidates and their televised debate had a prominent influence on the volume and content of communications, and that the content and emotional tone of communications more reflects preferences along the EU dimension of political contestation rather than classic national issues relating to left-right differences.     

Conduit or contributor? The role of media in policy change theory

The policy change literature is contradictory about the role the media plays in policy change: a conduit for policy participants, with media accounts transmitting multiple policy beliefs of those involved in policy debates or a contributor in the policy process, with media accounts supplying consistent policy beliefs with congruent narrative framing strategies to construct a policy story. The purpose of this study is to empirically test whether the role of the media is that of a conduit or contributor in the policy change process. This study tests whether there are differences in policy beliefs and narrative framing strategies between local and national print media coverage of two contentious policy issues in the Greater Yellowstone Area between 1986 and 2006, that of snowmobile access and wolf reintroduction. In the Greater Yellowstone Area policy arena, local media accounts are believed to be aligned with the Old West Advocacy Coalition, whereas the national media accounts are thought to be part of the New West Advocacy Coalition. With a methodology informed by narrative policy analysis, one hundred seventy five local and national print newspaper accounts were content analyzed to determine whether these media accounts were policy narratives, with embedded policy beliefs and narrative framing strategies. The results indicate that there are statistical differences between local and national media coverage for five of the seven hypotheses. Media accounts are generally policy stories, suggesting that the media’s role is more of a contributor than a conduit in the policy change process.

政治资源：涵义、特征与价值

政治资源是社会资源体系的一个重要组成部分,政治资源是指能够使政治行为主体对政治客体发生作用,从而影响政治变迁、维护政治稳定、推动政治发展的物质与非物质因素的总和。政治资源具有有效性、稀缺性、阶级性、流动性、可塑性等特点,在理论研究上和实践中都有重要价值。     

试论政策终结的内容

一般来说,政策终结内容可以划分为执行措施、政策本身、政策执行、政策机构和政策功能终结五种,它们在终结难易上具有一般的逻辑顺序关系;同时这些内容也存在着互相交叉的关系,所以具体应用的时候需要综合分析、科学决策。     

试论《经济、社会和文化权利国际公约》中的健康权

《经济、社会和文化权利国际公约》的健康权是一个综合性的概念，它指实现身体健康和心理健康的权利和自由。健康权具有多重性质，保护和实现健康权，必须考虑社会、经济生活因素和其他影响健康权实现的诸多因素。健康权与公民权利和政治权利之间存在密切的关系。     

中国互联网内容监管的预期与效果——事实及评价

从监管预期与效果的落差来看,不同时期的案例证明,中国互联网"国家防火墙"仍会有间歇性失常,无论是冲动许可、维权春天还是哄客暴戾,民众表达仍然有弹性空间。西方的政治家和知识精英基于他们的自由价值立场和生活经验,对中国互联网内容监管提出了强烈批评,辩护者则注意到中国政府的现实权衡。批评者表达的是私人自由与公共权力之间的紧张,辩护者关注的是公共权力与统治秩序之间的调和。     

思想政治工作必须与时俱进

在改革开放深入发展的新形势下，要使高校学生的思想政治工作跟上时代前进的步伐，政治辅导员的思想观念、思维方式要进一步更新，思想教育内容与思想教育方法也要与时俱进，以变应变。     

当代中西文化交流的新内容

党的十一届三中全会之后,我国实行了改革开放政策,同时全球化的浪潮席卷了全世界。主动开放的内部因素和全球化的外部压力,促使当代中西文化交流呈现出新的内容,主要表现为:中西政治机制上的借鉴创新、经济活动的跨国经营管理、中西文化艺术交流和日常生活中的中西文化交流——语言、互联网络、洋快餐、跨国婚姻等的多重变奏。