细粒棘球绦虫排泄分泌抗原研究——六钩蚴排泄分泌抗原及原头节可溶性粗抗原的SDS-PAGE

本文应用十二烷基硫酸钠—聚丙烯酰胺凝胶电泳(SDS-PAGE)首次对细粒棘球绦虫六钩蚴排泄分泌(ES)抗原的多肽组分进行了分析并同时对牦牛源棘球蚴原头节可溶性粗抗原的多肽组分进行了分析。应用10％凝胶,采用垂直板型电泳、考马斯亮蓝R-250染色进行SDS-PAGE的结果表明,六钩蚴ES抗原至少由14种多肽组分组成,分子量范围270～17.5KD,其中主要组分6种。原头节可溶性粗抗原至少由25种多肽组分组成,分子量范围230～17KD,其中主要组分13种。本项研究为功能性抗原的进一步分析、分离奠定了基础。     

大鼠液压冲击脑损伤bFGF及其受体FGFR1 mRNA表达

目的 观察脑损伤后 bFGF及其受体 FGFRl mRNA表达及其时序性变化,探讨脑损伤的分子机制及法医学脑损伤时间推断。方法 用原位杂交(ISH)和RT-PCR技术观察大鼠液压冲击脑损伤后bFGF及FGFRl mRNA在伤后不同时间(30min、1h、3h、6h、12h、1d、3d、7d)的表达情况,以正常大鼠及手术大鼠作为对照。结果(1)正常及手术对照组大鼠脑组织内有低水平的bFGF及FGFRl mRNA表达;(2)脑损伤后6h～3d,大脑皮质和脑干bFGF/FGFRl mRNA水平逐渐增加,7d时已有所下降,但仍未恢复到基础水平;(3)海马冲击后3h即开始显著增加,3d开始下降,7d时已恢复到基础水平。结论 脑损伤可诱导bFGF/FGFRl基因表达,机体对脑损伤存在自身保护机制,其表达的时序性改变可望用于法医学脑损伤时间推断。     

利用转基因植物生产口蹄疫可饲疫苗的研究进展与前景

从口蹄疫可饲疫苗的研究概况、生产技术要点、优缺点及需要改进的问题几方面阐述了利用植物反应器生产口蹄疫可饲疫苗的研究前景。综述了口蹄疫可饲疫苗的研究进展,介绍了利用植物反应器生产口蹄疫可饲疫苗的技术要点,包括在口蹄疫病毒遗传转化质粒载体系统中最常用的农杆菌介导法,提出了生产口蹄疫可饲疫苗需要改进的技术问题。     

人脑挫伤后细胞周期素D1表达的变化

目的研究CyclinD1在人不同部位脑挫伤组织中表达的变化及其与损伤时间的关系。方法88例脑挫伤标本按损伤后存活时间0.5,1,3,24h和3,7,14,30d分为8个实验组,另以6例非脑挫伤的脑作为对照组,应用CyclinD1免疫组织化学并结合图像分析技术观察CyclinD1的变化。结果脑挫伤后,挫伤灶中央CyclinD1阳性细胞几乎丧失,1h后挫伤灶周围CyclinD1阳性细胞开始增加,3h～30d之间各组挫伤灶周围免疫阳性反应的细胞增加显著,在3h～30d一直维持在较高水平;CyclinD1主要见于小胶质细胞和其它胶质细胞,少数神经元也呈阳性。结论人脑挫伤后,CyclinD1在多种脑细胞内表达,以胶质细胞表达明显,CyclinD1阳性细胞在伤后不久即显著增加,故可作为早期脑损伤的诊断指标。     

Genetic polymorphism at 15 tetrameric short tandem repeat loci in four aboriginal tribal populations of Bengal

POPULATIONS: This study reports the genetic polymorphism observed at 15 short tandem repeat loci D3S1358, TH01, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX, D2S1338, D19S433, and FGA in four aboriginal populations of Bengal. The analysis was performed to decipher the suitability of CODIS as well as six other highly polymorphic and unlinked markers in Forensic Testing. Studied populations include four tribes: Karmali, Kora, Maheli, and Lodha.     

GHB free acid: I. Solution formation studies and spectroscopic characterization by 1HNMR and FT-IR

In forensic evidence, gamma-hydroxybutyric acid (GHB) has frequently been encountered in one of its salt forms (gamma-hydroxybutyrate), but has also been encountered in its free acid form (GHB). Owing to the physical properties, encounters of the free acid have been largely restricted to forensic exhibits comprising aqueous solutions, such as acidic beverages that have been "spiked" or formulated with GHB salts or gamma-butyrolactone (GBL). The analysis of GHB free acid presents particular difficulties including the potential for altering the original proportions of GHB free acid, GHB carboxylate, and GBL in the course of analysis, and discrimination between GHB free acid and carboxylate forms. In this work, the formation of GHB free acid in aqueous solutions (water and/or D2O) was studied as a function of solution pH. Proton nuclear magnetic resonance (1HNMR) and Fourier-transform infrared spectrometry (FT-IR) measurements were obtained on freshly prepared mixtures of NaGHB and HCl stock solutions representing a series of points along the GHB titration curve. Both 1HNMR and FT-IR were shown to track the changing proportions of GHB free acid and carboxylate forms as a function of pH, while simultaneously monitoring for the formation of the lactone (GBL). The results were consistent with acid-base conversion behavior for a carboxylic acid. 1HNMR was shown to provide an ideal means for analysis of aqueous-based GHB/GBL forensic exhibits based on simple dilution of the neat liquid exhibit, without altering the original proportions of GHB free acid, carboxylate, and GBL in the samples.