LC-MS/MS-MRM筛选血液中22种有毒生物碱成分

目的建立液相色谱-串联质谱法对血液中的22种常见有毒生物碱成分进行筛选分析。方法血液以丁丙诺菲为内标经液液提取后,用液相色谱-串联质谱仪以电喷雾电离(ESI )、多反应监测(MRM)方式进行分析。结果以化合物的保留时间、两对母离子/子离子对定性,最低检出限为0.1～20ng/mL。结论该方法选择性佳、灵敏度高,适用于法医毒物分析和临床毒物分析中有毒生物碱的分析。     

精神病人限定刑事责任能力评定量表在强奸案件中的运用

目的探讨《精神病人限定责任能力评定量表》在强奸案件中的运用。方法用《精神病人限定责任能力评定量表》评定为限定刑事责任能力的77例强奸案例,完成了量表评定,同时将限定刑事责任能力进行三级划分。结果小部分组、部分组、大部分组量表评定分逐渐升高,且具有显著性差异,量表共16个条目与评定分相关,相关系数从0.265至0.611不等,因子分析得到7个因子,解释75.784%的变异。结论精神病人限定责任能力量表内部构建合理,在强奸案件中其评定总分能有效反映三级限定刑事责任能力之间差异。     

中国北方汉族群体TPH基因座-T457C位点遗传多态性

目的研究色氨酸羟化酶(tryptophan hydroxylase,TPH)基因座-T457C位点在中国北方汉族群体中的遗传多态性及其法医学应用价值。方法应用PCR-RFLP、聚丙烯酰胺凝胶电泳及银染技术,检测180例北方汉族无关个体TPH基因座-T457C位点的遗传多态性。结果TPH基因座-T457C位点在中国北方汉族群体中,其个体识别能力(DP)为0.624,非父排除率(PE)为0.187,与法国人群基因频率比较差异显著(P<0.05)。结论TPH基因座-T457C位点在法医学中有重要应用价值,其遗传多态性可能存在民族或地域差异。     

Rethinking the presumption of innocence

This article is concerned with what constitutes interference with the presumption of innocence and what justifications there might be for such interference. It provides a defence of a theory of the presumption of innocence that suggests that the right is interfered with if the offence warrants conviction of defendants who are not the intended target of the offence. This thesis is defended against two alternative theories. It then considers what might justify interference with the presumption of innocence. It explores the idea that interference is justified if it is necessary in a democratic society and considers the presumption in relation to the aims of the criminal trial. It is concluded that no good grounds have been provided for interference with the right, and that the right should be regarded as inviolable.

A New Method for Human ABO Genotyping Using a Universal Reporter Primer System

Abstract: We developed a new method for forensic ABO genotyping based on a universal reporter primer (URP) system. This allows for the simultaneous detection of six single nucleotide polymorphism (SNP) sites in the ABO gene (nucleotide positions 261, 297, 526, 703, 796, and 803). This URP system provides obvious peaks, ranging from 82 to 151 bp in length. ABO genotypes were classified and successfully genotyped by our method, including minor alleles that may cause a discrepancy between the genetic data and serological phenotypes. Full profiles were identified using as little as 0.1 ng (0.05 ng/reaction) of standard K562 and 9947A DNA. Moreover, the success rate of genotyping from a URP system was much higher than that from a conventional primer extension method in degraded DNA. This method enables simple and rapid detection of multiple SNP sites on human ABO genes and is highly specific and sensitive when using limited and degraded DNA.     

再论动产物权变动的生效要件

我国《物权法》第23条关于动产物权以交付为变动的生效要件的规定,统领各种类型的动产物权变动,其但书仅适用于动产抵押权、浮动抵押权、海上运输的指示单证及非基于法律行为的动产物权变动等极个别的情形。从物物权的变动、船舶、航空器和机动车诸物权变动,只要是基于法律行为的,依然以交付而非登记为生效要件。我国《物权法》第26条关于指示交付的界定过于狭窄,应予扩张。     

How to control interception-does the UK strike the right balance?

This Article critically analyses the regime for intercepting the content of communications under the Regulation of Investigatory Powers Act 2000 in the light of the recent ruling by the European Court of Human Rights in Kennedy v the UK. It looks at the safeguards for privacy protection provided such as the requirement for a warrant and the roles of the Investigatory Powers Tribunal and the Interception of Communications Commissioner and whether these safeguards are compliant with Article 8 of the European Convention of Human Rights.     

LC/MS分析血浆中丁丙诺啡

目的建立血浆中丁丙诺啡液相色谱/质谱（LC/MS）分析方法。方法在含有丁丙诺啡的血浆中,加入内标奋乃静,加pH10.8缓冲溶液,用401有机担体作吸附剂、三氯甲烷作洗脱剂固相萃取,N2挥干,用50μL流动相定容后进行LC/MS分析。色谱条件：Thermo Hypersil-HyPURITY C18（150×2.1mm,5μm）,柱温：40℃,流动相：10mmol/LNH4AC（pH3.4）∶甲醇∶乙腈=36∶52∶12,流速：0.22mL/min。结果方法的线性范围为0.05～5.0μg/L（r=0.9998）,定量限0.05μg/L,检出限0.01μg/L（S/N=3）;3个浓度的质量控制样品（0.1μg/L,0.5μg/L,2.0μg/L）平均回收率分别为86.40%,92.72%,92.57%,RSD分别为4.51%,3.34%,2.09%。结论该方法操作简便、灵敏度高,可用于涉毒案件血浆中丁丙诺啡的分析。     

The exploitation of Business Register data from a public sector information and data protection perspective: A case study

Business Registers (BRs) are a very important information resource for investors, creditors, financial institutions and public authorities. The possibility to aggregate and interconnect these data at a European level could enhance the transparency of companies towards those actors and add a great deal of value to the raw Business Register data. The European BRITE project intended to provide adequate tools to meet these demands. BRITE will provide easier access and cross-border interoperability of Business Register data throughout Europe. On the other hand, the processing of BR data within the BRs and BRITE triggers several important European legislations such as the Data Protection Directive and the Directive on the re-use of public sector information. In this paper, the processing of BR data will be analysed from the perspective of both data protection and public sector information laws, analysing as well the relation between both regulations. Do these regulations strike an optimal balance between the interests of private data vendors to re-use BR data and enhance business transparency and the need to protect the personal data of natural persons?     

An initial evaluation of stable isotopic characterisation of post-blast plastic debris from improvised explosive devices

A number of two-way radios, similar to those which have been employed to initiate Improvised Explosive Devices (IEDs), were acquired from a commercial supplier and grouped into four pairs. Samples of plastic material were collected from five distinct regions of each radio and analysed by Infrared and Raman spectroscopy to identify the nature of the material. One radio of each pair was then subjected to detonation with a commercially available plastic explosive. The combination of radio and explosive was considered to be representative of the components of an IED. Following detonation, fragments were recovered and, where possible, identified as specific sampling points of the radio.A combination of δ²H and δ¹³C stable isotopic analysis of material from each of the five sampling points was found to provide a pattern which was characteristic of a given radio and provided a means to associate pairs of radios. When few fragments were recovered, no positive association could be made between the fragments and the paired, undamaged radio. This was attributed, in part, to manufacturing variation in the radios. However, when three or more post-blast fragments were recovered it was possible to associate these with the paired, undamaged radio with a high degree of certainty.