腭缝的年龄变化

作者对231具中国汉族成年男性的干燥颅骨腭缝的年龄变化进行了分析研究。发现腭缝的愈合随着年龄的增加而有规律地增加。并且在45岁以前更加明显。通过对汉族成年男性腭缝愈合与年龄变化关系的研究,从中发现了腭缝愈合年龄变化的初步规律,为骨骼年龄变化的判断提出了一个新的方法。     

分泌抗人精子单克隆抗体杂交瘤细胞株的建立及鉴定

建立两株分泌抗人精子单克隆抗体的杂交瘤细胞株G_8与E_(10),采用洗涤人精子免疫Balb/c小鼠,取免疫脾与Balb/c小鼠的Sp2/0骨髓瘤细胞融合,杂交瘤细胞诱生腹水的方法。Elisa,IIF或酶标抗体免疫组化法结果发现,G_8与E_(10)单抗与人精子(射出精子、睾丸、副睾管及输精管中的精子)及睾丸各级生殖细胞均发生反应;与人甲状腺上皮细胞、肾小管上皮细胞、胰腺导管上皮细胞以及胰岛均发生不同程度的交叉反应,与其他33种人体正常组织、9种人体液与分泌液、8种不同种族的动物精子均不发生交叉反应,说明在一般情况下这两种抗体可用于鉴定人类的精液斑以及精液与阴道分泌液的混合斑。两种单抗与人精子的顶体部、赤道部、后核帽、颈部及中段结合,证明精子特异性表面抗原分布于这几个区域。两种抗体均属IgG_1,G_8与E_(10)抗体的效价分别为1:512与1:1024。     

ELISA检测精浆特异蛋白P_(30)鉴定人精斑

<正> 1978年美国Sensabaugh分离出精浆特异蛋白P30,并成功的制备出相应的抗P30血清。1987年我国也研制出抗人精浆特异蛋白P30血清,并应用该种抗血清建立了几种琼脂扩散和电泳方法鉴定人精斑。由于人精液中P30含量不高,平均为1.52±0.676mg/ml,鉴定微量精斑存在困难。为了更有效的提高方法的灵敏度。我们建立了鉴定微量人精斑的ELISA固相P30抗体法,现报告如下。     

“入世”与中国的反垄断立法

针对“入世”的形势和国内一些学者的模糊认识 ,本文通过大量的实证分析指出 ,制订《反垄断法》无论从国际层面还是从国内层面均属必需 ,而且 ,立法需考虑这两方面的问题。在此基础上 ,笔者对《反垄断法》的核心问题即垄断的认定问题作了较详细的分析。     

High-throughput DNA sequencing of environmentally insulted latent fingerprints after visualization with nanoscale columnar-thin-film technique

The goals of this study were to (a) ascertain human identity capabilities of DNA obtained from latent fingerprints that have been first environmentally insulted and then developed by the deposition of a columnar thin film (CTF), and (b) to determine if the CTF process and material are detrimental to single nucleotide polymorphism (SNP) analysis. Fingerprints were deposited on five different types of substrates and aged for one day, 7 days or 30 days while being environmentally insulted under one of the four conditions: 16.6 °C and 60% relative humidity (RH) (Condition A), 24.5 °C and 60% RH (Condition B), 35 °C and 67% RH (Condition C) and a cold condition (Condition D). Then CTF technique was then on 59% of these fingerprints. DNA samples from 805 fingerprints were extracted, quantified, subjected to manual library preparation using the Precision ID Identity Panel, and underwent high-throughput sequencing. The Ion S5™ platform was employed to sequence 124 SNP amplicons. SNPs were successfully sequenced from 802/805 samples. Total read depth was consistent across environmental conditions, and majority of samples had 100% profile completeness and 100% concordance. Anecdotally, libraries that were amplified with a higher cycle number had more ‘Major Allele Frequency’ flags compared to samples amplified with 23 cycle numbers, possibly due to stochastic effects. Neither the substrates nor the CTF process and materials inhibit downstream DNA analysis. DNA of low quality and quantity from the chosen samples can be sequenced using the Precision ID Identity Panel on the Ion S5™ platform which performed well, however, a different approach may be needed if spurious alleles are suspected.     

Interpretation,confidence and application of the standardised terms: Identified,Probable, Possible,Exclude and Insufficient in forensic odontology identification

Forensic odontology identification scales are used to express certainty of identifications of deceased persons. These standardized scales are assumed to convey unambiguous expert opinions and facilitate communication between forensic odontologists and end users. However, to date no studies have investigated how the experts interpret and use these scales.Forensic odontology identification scales are used to express certainty of identifications of deceased persons. These standardized scales are assumed to convey unambiguous expert opinions and facilitate communication between forensic odontologists and end users. However, to date no studies have investigated how the experts interpret and use these scales.This paper aims to examine the interpretation of the DVISYS forensic identification scale and choices of the levels in the scale subsequent to, and derived from, comparison of pairs of dental radiographs by extending the analysis of the data collected in the study by Page and Lain et. al. 2017.The studied variables: self-reported confidence, forced binary decision of match and non-match, choice of level in the DVISYS scale (Identified, Probable, Possible, Insufficient and Exclude) were further analysed in this study using mixed models for relationships between the choices of level in the identification scale and the fundamental beliefs of likelihood of identification.The results of this further analysis showed that the reported confidence of the decisions was correlated to the difficulty of cases, and as confidence decreased the use of less definitive terms (‘Probable’, ‘Possible’ and ‘Insufficient’) increased. ‘Probable’ and ‘Possible’ were used mainly in underlying beliefs below that of ‘Identified’ whereas ‘Insufficient’ was used mainly to convey a sublevel of ‘Exclude’. The use of ‘Insufficient’ in this study was not consistent with the prescribed definition of the term.The participants of the original study were not aware of the difficulty grading of the cases nor were required to grade them, however the reported confidence was systematically correlated to difficulty. Furthermore, indicated confidence level was correlated with choice of level on the scale in general, but the interpretation of the definition and application of the terms varied.The findings reported here contribute to the foundational knowledge of factors governing the interpretation and application of the DVISYS forensic odontology identification scale and suggest that this scale may need to be modified.     

元明清王朝治回政策与回族形成

从唐宋元王朝的回族先民到明清王朝的回族,每个王朝的统治者都制定了不同的政策,这些政策的制定与回族的形成有着密切关系。在元代恩威相济的对回政策下,回族共同体雏形形成;明代回回人仍受重用,对回实行优待和强制同化政策,回回人融合汉族文化进而加速了原文化的本土化进程,回族共同体形成;清代对回政策前后有别,前期因俗而治,后期采取“以回制回”的压制政策,回族共同体非但未被削弱,民族认同得到加强。     

医疗损害责任纠纷案件鉴定问题研究——以2018年江苏省审判数据为依据

目的:对医疗损害鉴定问题进行实证研究,探讨医疗损害鉴定实施现状与责任认定情况,为完善我国医疗损害鉴定制度提供依据.方法:采用SPSS22.0软件对2018年江苏省医疗损害责任审判案件进行统计学分析.结论:医疗损害鉴定优先选择医学会鉴定;医疗损害"二元化"鉴定模式并存损害司法公平正义;重复鉴定、多重鉴定增加诉讼成本.对策...     

正常、大声两种说话状态下语音的声学差异及对声纹鉴定的影响

本文主要通过对正常、大声两种说话状态下的普通话中三个单元音[a]、[i]、[u]的声强、时长、基频、谐波振幅差值、共振峰等声学参数的分析,综合比较了各参数的变化规律,发现大声说话时的语音并非正常语音的简单放大,二者不仅在声强上存在差别,同时在频率域上也发生了重要变化。同一人不同状态下发音的频谱特征差异性较大,同种状态下发音的相似性、可比性较强,为此,声纹鉴定中应尽量选取状态相同的语音进行比对。     

东北亚地区的民族国家认同、地区认同与对外认知

认同会对政治的认识与行为带来重要影响。但从国际关系视角对这一问题进行研究尚处于起步阶段。本文就构建东北亚共同体为背景,以韩中日三国为主轴,通过问卷调查的形式,对东北亚地区民族国家认同的强度和影响力,民族国家认同对地区认同的形成以及东北亚国家的对外认识的影响,超国家的接触对国家、地区认同及对外认识的影响等三个问题得出了结论。并认为,韩中日三国均表现出强烈的国家认同,并对地区共同体的形成产生不良影响;东北亚地区存在的否定相互认知,根源在于日本帝国主义的侵略战争。