A new peptidase isozyme which may assist in the identification of vaginal debris

A characteristic peptidase has been identified in vaginal swab extracts. This enzyme, which has been termed vaginal peptidase, can be identified by its high anodal electrophoretic mobility in starch gel at pH 7.4 and its ability to hydrolyse the dipeptide substrate L-valyl-L-leucine. Vaginal peptidase was not detected in any of the body fluids or excretions commonly encountered in forensic stain analysis, other than vaginal debris. The use of this enzyme as a biochemical marker for the identification of vaginal debris in dried stains is discussed.     

重视个体、寻求双赢的组织人本管理模式

一项调查结果显示,员工的组织认同心理具有较强的依赖性,组织认同心理与组织公民行为之间不存在显著相关,这说明“单位制”管理整合机制在市场经济条件下必然带来组织认同危机。相关理论和实践表明,只有以组织和员工双赢为出发点的组织管理整合才能激发真正的员工组织认同,员工持股制度则是促进员工组织认同和增强组织管理整合有效性的重要制度。     

现代政治认同与和谐社会

现代政治认同作为公民对于政治共同体、政府及其基本制度的认可与支持,是一种积极的、理性的、多层次的政治认同。它是维系现代国家中民族团结、社会稳定与和谐的政治心理基础,因而对于建设社会主义和谐社会具有十分重要的意义。解决此问题对于和谐社会的建构极为重要。     

国际私法中的识别问题初探

识别是国际私法的一个基本问题。在涉外民事案件的处理中,识别冲突是普遍存在的现象。识别冲突具有多方面的成因,解决识别;中突的理论也各有千秋,但也均有不足之处。识别问题不仅对适用冲突规范具有意义,而且对确定管辖也有影响。     

Multiplex-direct PCR assay for foodborne pathogen identification: An application in forensic investigation

Foodborne pathogens present serious concerns to human health and can even lead to fatalities. Microbial forensic science thus plays an important role in consumer protection, food security, and even in litigation. The gold standard for pathogen identification – bacterial culture – is costly and time-consuming. A cheaper and quicker alternative will benefit both forensic science and medical diagnosis. In this study, we developed and validated a molecular-based method termed ‘multiplex-direct PCR assay’ to simultaneously detect three common foodborne pathogens – Escherichia coli O157:H7, Campylobacter jejuni, and Listeria monocytogenes. Three previously reported species-specific primer pairs were modified and used to directly amplify samples without DNA extraction. The assay was also validated for its specificity, sensitivity, and applied to test several samples obtained from a local market and clinical samples. The results showed the expected PCR fragments of approximately 490, 343, and 209 bp for E. coli O157:H7, C. jejuni, and L. monocytogenes, respectively. The assay was specific to the targeted pathogens and was sufficiently sensitive and robust to effectively analyze market samples. The whole process took less than 1 h to complete indicating that the assay is suitable for reliable, rapid, and inexpensive identification of these three foodborne pathogens, which could be useful in microbial forensic investigation.     

根据肋骨推断年龄的初步研究

肋骨和全身其他骨一样,其形态一生都在变化.Kerley发现,青春期的肋骨胸骨端呈波浪状;中年期的呈杯状,有锐利的边缘;老年期的呈不规则状.最近,Iscan等根据Kerley的初步观察,把肋骨胸骨端作为测定年龄的又一可以实践的部位,先后用两种方法对其形态改变进行了分析.他们最初用的是成分分析法(component analysis),后来用的是阶段分析法(Phase analysis).iscan等认为,阶段分析     

Anonymisation of personal data – A missed opportunity for the European Commission

As early as the 1970's, privacy studies recognised that ‘anonymisation’ needed to be approached with caution. This caution has since been vindicated by the increasing sophistication of techniques for reidentification. Yet the courts in the UK have so far only hesitatingly grappled with the issues involved, while European courts have produced no guidance.     

生物识别技术在警务工作中的应用

为了维护社会治安的正常秩序,警务工作要建立有效社会控制机制,应用生物识别技术防范及处置影响社会稳定的案件和事件。生物识别技术发展迅速,在警务工作应用广泛。     

毒品检验鉴定程序的原则

毒品检验鉴定是我国禁毒工作中十分重要的一部分,开展毒品检验鉴定工作的目的是为了在涉毒刑事案件中,能够及时、准确地对查获的毒品疑似物进行定性、定量分析,为毒品案件的侦破和审理提供证据支持。程序的规范化能够保证毒品检验鉴定工作的科学性和准确性,在为打击毒品犯罪提供证据支持的同时,维护了国家司法的公正和权威。毒品检验鉴定程序...     

生存现状、话语演变和异质的声音——90年代以来的同性恋研究

90年代末以来,在酷儿全球化趋势下,中国同性恋从疾病、变态、政治上不正确的性关系转变成非病理化和非罪化的主体间人格关系。作为现代性后果的中国同性恋话语在20世纪经历了从包容-抑制-重新包容的演变过程。然而,当前的同性恋叙述存在话语垄断现象,主体自主性的话语、知识和声音被总体化的宏大叙事所压制,这种压制不仅来自异性恋社会,也来自同性恋社区本身。近20年的同性恋研究大致经历了从行为到身份、从本质论到建构论的转变。