利用转基因植物生产口蹄疫可饲疫苗的研究进展与前景

从口蹄疫可饲疫苗的研究概况、生产技术要点、优缺点及需要改进的问题几方面阐述了利用植物反应器生产口蹄疫可饲疫苗的研究前景。综述了口蹄疫可饲疫苗的研究进展,介绍了利用植物反应器生产口蹄疫可饲疫苗的技术要点,包括在口蹄疫病毒遗传转化质粒载体系统中最常用的农杆菌介导法,提出了生产口蹄疫可饲疫苗需要改进的技术问题。     

人脑挫伤后细胞周期素D1表达的变化

目的研究CyclinD1在人不同部位脑挫伤组织中表达的变化及其与损伤时间的关系。方法88例脑挫伤标本按损伤后存活时间0.5,1,3,24h和3,7,14,30d分为8个实验组,另以6例非脑挫伤的脑作为对照组,应用CyclinD1免疫组织化学并结合图像分析技术观察CyclinD1的变化。结果脑挫伤后,挫伤灶中央CyclinD1阳性细胞几乎丧失,1h后挫伤灶周围CyclinD1阳性细胞开始增加,3h～30d之间各组挫伤灶周围免疫阳性反应的细胞增加显著,在3h～30d一直维持在较高水平;CyclinD1主要见于小胶质细胞和其它胶质细胞,少数神经元也呈阳性。结论人脑挫伤后,CyclinD1在多种脑细胞内表达,以胶质细胞表达明显,CyclinD1阳性细胞在伤后不久即显著增加,故可作为早期脑损伤的诊断指标。     

Genetic polymorphism at 15 tetrameric short tandem repeat loci in four aboriginal tribal populations of Bengal

POPULATIONS: This study reports the genetic polymorphism observed at 15 short tandem repeat loci D3S1358, TH01, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX, D2S1338, D19S433, and FGA in four aboriginal populations of Bengal. The analysis was performed to decipher the suitability of CODIS as well as six other highly polymorphic and unlinked markers in Forensic Testing. Studied populations include four tribes: Karmali, Kora, Maheli, and Lodha.     

GHB free acid: I. Solution formation studies and spectroscopic characterization by 1HNMR and FT-IR

In forensic evidence, gamma-hydroxybutyric acid (GHB) has frequently been encountered in one of its salt forms (gamma-hydroxybutyrate), but has also been encountered in its free acid form (GHB). Owing to the physical properties, encounters of the free acid have been largely restricted to forensic exhibits comprising aqueous solutions, such as acidic beverages that have been "spiked" or formulated with GHB salts or gamma-butyrolactone (GBL). The analysis of GHB free acid presents particular difficulties including the potential for altering the original proportions of GHB free acid, GHB carboxylate, and GBL in the course of analysis, and discrimination between GHB free acid and carboxylate forms. In this work, the formation of GHB free acid in aqueous solutions (water and/or D2O) was studied as a function of solution pH. Proton nuclear magnetic resonance (1HNMR) and Fourier-transform infrared spectrometry (FT-IR) measurements were obtained on freshly prepared mixtures of NaGHB and HCl stock solutions representing a series of points along the GHB titration curve. Both 1HNMR and FT-IR were shown to track the changing proportions of GHB free acid and carboxylate forms as a function of pH, while simultaneously monitoring for the formation of the lactone (GBL). The results were consistent with acid-base conversion behavior for a carboxylic acid. 1HNMR was shown to provide an ideal means for analysis of aqueous-based GHB/GBL forensic exhibits based on simple dilution of the neat liquid exhibit, without altering the original proportions of GHB free acid, carboxylate, and GBL in the samples.     

“9＋2”与“10＋1”框架机制比较

中国—东盟的全面经济合作,是应经济全球化而产生的国际区域合作的又一新的成果,其“合作框架协议”是最基本的法制依据。而我国南方九省区加上香港、澳门两地的“泛珠三角区域合作”则是在一个国家之内,“一国两制”之下不同省区横向合作的机制创新,它不仅受到了国际区域经济合作创新的启示,而且顺应了整个社会区域整体发展的潮流。本文将在二者的实施框架机制方面进行一定的比较,欲为“泛珠三角区域合作”的法制建构献可替否。     

对马克思主义人的全面发展理论的深刻论述——学习江泽民同志“七一”讲话的一点体会

江泽民同志的“七一”讲话 ,站在时代的高度 ,对马克思主义人的全面发展理论作了深刻论述 ,实现了理论上的重大创新 :讲话将人的全面发展提到了科学的高度 :“努力促进人的全面发展 ,这是马克思主义关于建设社会主义新社会的本质要求。”讲话对人的全面发展的内涵作了全面论述和深刻揭示。讲话指出了推进人的全面发展的正解途径。     

Pflichtteilsverkürzung durch Einbringung des Vermögens in eine Stiftung / Beginn der Zweijahresfrist nach § 785 Abs 3 ABGB

Auch Zuwendungen an Privatstiftungen k?nnen nach ganz herrschender Meinung Schenkungen iSd § 785 ABGB sein, selbst wenn die Zuwendung zugleich mit dem einmaligen Stiftungsakt erfolgt. Im Schutz vor einer Wertvernichtung durch Verm?gensverteilung liegt keine Rechtfertigung dafür, das – bei Schaffung des PSG unangetastet gebliebene – Pflichtteilsrecht mit Hilfe einer Stiftung "auszuhebeln". Unabh?ngig davon, welcher der in der Literatur vertretenen Meinungen zur Nichtausl?sung der Zwei-Jahres-Frist des § 785 Abs 3 Satz 2 ABGB im Einzelnen gefolgt würde, bewirkt der Umstand, dass in der Stiftungserkl?rung ein umfassender ?nderungsvorbehalt zugunsten des Stifters und ein Widerrufsvorbehalt des Stifters vorgesehen sind (wobei beide Vorbehalte auch für Stiftungszusatzurkunden gelten), dass dem Stifter noch so wesentliche Einflussm?glichkeiten auf das Stiftungsverm?gen verbleiben, dass das von § 785 ABGB geforderte Verm?gensopfer noch nicht als erbracht anzusehen ist. Auf eine Umgehungsabsicht kommt es nicht an.     

Comparison of two methods for isolating DNA from human skeletal remains for STR analysis

Abstract: The quality and efficiency of a standard organic DNA isolation method and a silica‐based method using the QIAGEN Blood Maxi Kit were compared to obtain human DNA and short tandem repeats (STRs) profiles from 39 exhumed bone samples for paternity testing. DNA samples were quantified by real‐time PCR, and STR profiles were obtained using the AmpFlSTR^® Identifiler^® PCR amplification kit. Overall, the silica‐based method recovered less DNA ranging from 0 to 147.7 ng/g (average 7.57 ng/g, median = 1.3 ng/g) than did the organic method ranging from 0 to 605 ng/g (average 44.27 ng/g, median = 5.8 ng/g). Complete profiles (16/16 loci tested) were obtained from 37/39 samples (95%) using the organic method and from 9/39 samples (23%) with the silica‐based method. Compared with a standard organic DNA isolation method, our results indicate that the published silica‐based method does not improve neither the quality nor the quantity of DNA for STR profiling.