制备抗人精液特异蛋白P30血清的研究

作者以精浆特异蛋白P30为抗原免疫新西兰白兔、豚鼠和鸡三种实验动物,制备了抗P30血清。用双向琼脂扩散法检测兔和豚鼠的抗 P30血清,其特异性和敏感性均达到目前国外同类产品的水平。抗 P30血清与阴道分泌物,血清、唾液、尿液、初乳以及羊精液、鸡精液均不出现交叉反应。用抗 P30血清检测混合的人精浆,其抗原效价为1:160;P30含量可测到12.5ug/ml。在三种动物的抗血清中,豚鼠抗 P30血清的抗体效价最高。以不同浓度的 P30测豚鼠、兔和鸡抗 P30血清抗体效价豚鼠平均滴度可达52.50,兔次之,鸡的抗 P30血清最差。经作者制备的抗 P30血清可用来确证精液。     

Footwear-to-feet examination and analysis: Comparing worn footwear to persons and human remains

Footwear-to-feet examination and analysis refers to the process of examining and analyzing footwear in regard to criminal matters. This examination and analysis has been done to include or exclude an individual as the wearer of the footwear in question and to help associate or disassociate human remains to footwear. This review of the subject describes its investigative applications, the research that underpins the process, and considers its legal admissibility. The process of footwear-to-feet examination and analysis is scientifically valid and has been widely accepted. As a result, law enforcement and forensic scientists should be aware of the use of this process to assist in criminal investigations.     

金钱白花蛇的微性状鉴别

目的 对目前药材市场上流通的金钱白花蛇及其伪品进行鉴别。 方法 利用性状鉴别法对金钱白花蛇及伪品进行性状鉴别,利用微性状鉴别法对金钱白花蛇及伪品的鳞片特征进行鉴别。 结果 4个样品中,仅一种样品为金钱白花蛇,其他3种样品为赤练蛇,其中2种样品为黑漆染色的赤练蛇。 结论 微性状鉴别法可以用于金钱白花蛇的真伪鉴别,市场上流通的金钱白花蛇造假严重,危及患者用药安全。     

正常、大声两种说话状态下语音的声学差异及对声纹鉴定的影响

本文主要通过对正常、大声两种说话状态下的普通话中三个单元音[a]、[i]、[u]的声强、时长、基频、谐波振幅差值、共振峰等声学参数的分析,综合比较了各参数的变化规律,发现大声说话时的语音并非正常语音的简单放大,二者不仅在声强上存在差别,同时在频率域上也发生了重要变化。同一人不同状态下发音的频谱特征差异性较大,同种状态下发音的相似性、可比性较强,为此,声纹鉴定中应尽量选取状态相同的语音进行比对。     

New perspectives in the use of ink evidence in forensic science: Part III: Operational applications and evaluation

The research reported in this series of article aimed at (1) automating the search of questioned ink specimens in ink reference collections and (2) at evaluating the strength of ink evidence in a transparent and balanced manner. These aims require that ink samples are analysed in an accurate and reproducible way and that they are compared in an objective and automated way. This latter requirement is due to the large number of comparisons that are necessary in both scenarios. A research programme was designed to (a) develop a standard methodology for analysing ink samples in a reproducible way, (b) comparing automatically and objectively ink samples and (c) evaluate the proposed methodology in forensic contexts.This report focuses on the last of the three stages of the research programme. The calibration and acquisition process and the mathematical comparison algorithms were described in previous papers [C. Neumann, P. Margot, New perspectives in the use of ink evidence in forensic science—Part I: Development of a quality assurance process for forensic ink analysis by HPTLC, Forensic Sci. Int. 185 (2009) 29–37; C. Neumann, P. Margot, New perspectives in the use of ink evidence in forensic science—Part II: Development and testing of mathematical algorithms for the automatic comparison of ink samples analysed by HPTLC, Forensic Sci. Int. 185 (2009) 38–50].In this paper, the benefits and challenges of the proposed concepts are tested in two forensic contexts: (1) ink identification and (2) ink evidential value assessment. The results show that different algorithms are better suited for different tasks. This research shows that it is possible to build digital ink libraries using the most commonly used ink analytical technique, i.e. high-performance thin layer chromatography, despite its reputation of lacking reproducibility. More importantly, it is possible to assign evidential value to ink evidence in a transparent way using a probabilistic model. It is therefore possible to move away from the traditional subjective approach, which is entirely based on experts’ opinion, and which is usually not very informative.While there is room for the improvement, this report demonstrates the significant gains obtained over the traditional subjective approach for the search of ink specimens in ink databases, and the interpretation of their evidential value.     

Criminal and alcohol problems among Swedish drunk drivers--predictors of DUI relapse

The prevalence and types of crime offences, as well as predictors of relapse, among drivers suspected of driving under influence (DUI) were investigated. A total of 1830 Swedish DUI drivers responded to the Alcohol Use Disorders Identification Test — AUDIT. Information about previous DUI offences, other traffic offences along with other types of criminal offences was taken from a crime register. A total criminality (including all traffic offences) of 64% in the period of five years before investigation was analyzed. 40% of the sample had other criminality besides traffic violations during that period. 14.3% of the drivers relapsed to DUI in the two-year period after the investigation. In terms of DUI relapse, the following factors were the main predictors: previous traffic violations, previous DUI offences, previous other criminality (frauds or other acts of dishonesty) and detection hours between 12.00 and 19.00. Detection in general traffic controls and high BAC (blood alcohol concentration) when detected were the strongest factors with negative correlation to DUI re-offence.     

网络犯罪的原因及对策

人类已进入信息社会,在信息技术、信息产业突飞猛进的同时,网络空间的计算机犯罪案件每年以几倍或几十倍速度增加,造成的损害远大于现实空间的犯罪。因此,防止网络犯罪显得十分重要。     

Developmental Validation of the ANDE 6C System for Rapid DNA Analysis of Forensic Casework and DVI Samples

A developmental validation was performed to demonstrate reliability, reproducibility, and robustness of the ANDE Rapid DNA Identification System for processing of crime scene and disaster victim identification (DVI) samples. A total of 1705 samples were evaluated, including blood, oral epithelial samples from drinking containers, samples on FTA and untreated paper, semen, bone, and soft tissues. This study was conducted to address the FBI’s Quality Assurance Standards on developmental validation and to accumulate data from a sufficient number of unique donors and sample types to meet NDIS submission requirements for acceptance of the ANDE Expert System for casework use. To date, no Expert System has been approved for such samples, but the results of this study demonstrated that the automated Expert System performs similarly to conventional laboratory data analysis. Furthermore, Rapid DNA analysis demonstrated accuracy, precision, resolution, concordance, and reproducibility that were comparable to conventional processing along with appropriate species specificity, limit of detection, performance in the presence of inhibitors. No lane-to-lane or run-to-run contamination was observed, and the system correctly identified the presence of mixtures. Taken together, the ANDE instrument, I-Chip consumable, FlexPlex chemistry (a 27-locus STR assay compatible with all widely used global loci, including the CODIS core 20 loci), and automated Expert System successfully processed and interpreted more than 1200 unique samples with over 99.99% concordant CODIS alleles. This extensive developmental validation data provides support for broad use of the system by agencies and accredited forensic laboratories in single-source suspect-evidence comparisons, local database searches, and DVI.     

Memory, Empathy, and the Politics of Identification

This essay explores the ethical and political dimensions of what I have elsewhere called “prosthetic memories” (Landsberg, Prosthetic memory: The transformation of American remembrance in the age of mass culture, Harvard University Press, 2004), focusing on those that are produced and disseminated cinematically. I argue that cinematic technology, by which I mean also to include the dominant cinematic conventions and practices used in the Hollywood style of filmmaking, is an effective means for structuring vision. Through specific techniques of shooting and editing, films attempt to position the viewer in highly specific ways in relation to the unfolding narrative. Sometimes, in such films, viewers are brought into intimate contact with a set of experiences that fall well outside of their own lived experience and, as a result, are forced to look as if through someone else’s eyes, and asked to remember those situations and events as both meaningful and potentially formative. By engaging specific strategies intended to elicit identification, films can force viewers to engage both intellectually and emotionally with another who is radically different from him or herself. This complicated form of identification across difference might condition viewers to see and think in ways that could foster more radical forms of democracy aimed at advancing egalitarian social goals.

败酱属6种药用植物的生药学鉴别

目的对败酱科败酱属6种同属药用植物的原植物及其根药材进行鉴别。方法采用性状鉴别法、微性状鉴别法和显微鉴别法对6种植物的根、茎、花序梗、花和果实等部位进行比较鉴别。结果败酱无果苞,花序梗单侧具糙毛,簇晶多成排或散聚于细胞中;攀倒甑、斑花败酱茎具两列糙毛,花白色或黄白相间,簇晶少;少蕊败酱无根茎花序梗具柔弱糙毛,簇晶多;异叶败酱花序梗被短糙毛,石细胞多且层纹明显;岩败酱果苞常具3主脉,花序梗被微糙毛,石细胞少。结论通过性状、微性状和显微特征可以鉴别败酱属6种药用植物。