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11.
The Application of Flow Cytometry as a Rapid and Sensitive Screening Method to Detect Contamination of Vitreous Humor Samples and Avoid Miscalculation of the Postmortem Interval 下载免费PDF全文
Cristina Cordeiro M.Sc. Rafael Seoane Ph.D. Ana Camba M.Sc. Elena Lendoiro M.Sc. María S. Rodríguez‐Calvo Ph.D. Duarte N. Vieira Ph.D. José I. Muñoz‐Barús Ph.D. 《Journal of forensic sciences》2015,60(5):1346-1349
Research into maximizing the speed, precision, and reliability of estimating the postmortem interval (PMI) has been a recurring object of investigation and methodologies based on the vitreous humor (VH) have provided good results. However, contamination from causes not readily apparent, such as blood, can occur, and thus lead not only to an erroneous estimation of PMI, but also interfere with the correct identification of other substances in the VH. We have developed a flow cytometry method which quantifies blood contamination and is able to detect erythrocytes in 1:750,000 dilution of contaminated VH which affects the results of hypoxanthine. It is an improvement on the previous more complex mass spectrometry method, being faster, more sensitive, and readily available. As such, it could be proposed for the rapid screening of appropriate samples by detecting and eliminating blood contaminated samples from PMI estimation. 相似文献
12.
Michael A. Busseri Teena Willoughby Heather Chalmers 《Journal of youth and adolescence》2007,36(3):279-289
A large volume of research has investigated interrelations among adolescent risk behaviors. Although several theoretical accounts
have been proposed, researchers have not directly examined hypotheses for why risk behaviors are linked. In the present paper, a distinction is drawn between predictive factors that explain variance
in risk behaviors and “linkage factors” which may provide an explanation for why risk behaviors are interrelated. The relevance
of linkage factors to risk behavior research, theory, and practice is described. Further, a simple to use and easy to interpret
analytic technique for exploring linkage-related issues is illustrated. Using this technique, hypotheses regarding the role
of predictors in explaining linkages among risk behaviors can be tested directly. The proposed line of inquiry will provide
valuable input for intervention efforts and theoretically relevant information concerning linkages among adolescent risk behaviors.
Research Associate at the Brock Research Institute for Youth Studies at Brock University, Ontario, Canada. He received his
M.A. in Clinical Psychology from the University of North Dakota. His major research interests are adolescent risk behavior
involvement and youth activity involvement as a context for positive development.
Professor in the Department of Child and Youth Studies at Brock University, Ontario, Canada. She received her Ph.D. in Developmental
Psychology from the University of Waterloo. Her major research interests include adolescent risk taking and resilience, including
academic underachievement and media/technology influences on lifestyle choices and learning.
Assistant Professor in the Department of Child and Youth Studies at Brock University, Ontario, Canada. She received her Ph.D.
in Developmental Psychology from Brock University. Her major research interests are adolescent risk-behavior involvement,
particularly related to gambling, and risk and resilience. 相似文献
13.
Despite the call to address domestic violence along with child maltreatment, little information exists to guide services for
victimized women involved with child welfare. Research shows that victimized women contend with multiple problems stemming
from violent victimization. Unfortunately less is known about combinations of needs and resources among victimized women,
especially for those involved with child welfare. Through an examination of needs and resources among families with child
maltreatment and domestic violence, we aimed to help address this knowledge gap. Needs and resources among 1,229 victimized
caregivers were examined using the National Survey of Child and Adolescent Well-being (NSCAW). Using latent class analysis,
four distinct multivariate profiles of needs and resources among victimized caregivers were identified. Significant differences
were found among the profile groups in the family violence they experienced in the 18 months following child welfare investigations.
Based on these findings, we discuss implications for tailored practices to enhance the safety of victimized caregivers involved
with child welfare.
An earlier version of this paper was presented at the 9th Annual Conference of the Society for Social Work and Research, Miami,
Florida in January 2005. 相似文献
14.
延安精神是中国共产党在延安时期培育出来的。延安精神是在马克思主义、毛泽东思想指导下形成和发展的,但中国传统文化对延安精神的形成和发展同样有着深刻的影响。它们之间是继承与批判的关系,延安精神是民族精神与时代精神的汇流。 相似文献
15.
Brett A. Schweers Jennifer Old P.W. Boonlayangoor Karl A. Reich 《Forensic Science International: Genetics Supplement Series》2008,2(3):243-247
Human blood is the body fluid most commonly encountered at crime scenes, and blood detection may aid investigators in reconstructing what occurred during a crime. In addition, blood detection can help determine which items of evidence should be processed for DNA-STR testing. Unfortunately, many common substances can cause red-brown stains that resemble blood. Furthermore, many current human blood detection methods are presumptive and prone to false positive results. Here, the developmental validation of a new blood identification test, Rapid Stain Identification™-Blood (RSID™-Blood), is described. RSID™-Blood utilizes two anti-glycophorin A (red blood cell membrane specific protein) monoclonal antibodies in a lateral flow strip test format to detect human blood. We present evidence demonstrating that this test is accurate, reproducible, easy to use, and highly specific for human blood. Importantly, RSID™-Blood does not cross-react with ferret, skunk, or primate blood and exhibits no high-dose hook effect. Also, we describe studies on the sensitivity, body fluid specificity, and species specificity of RSID™-Blood. In addition, we show that the test can detect blood from a variety of forensic exhibits prior to processing for DNA-STR analysis. In conclusion, we suggest that RSID™-Blood is effective and useful for the detection of human blood on forensic exhibits, and offers improved blood detection when compared to other currently used methods. 相似文献
16.
司法知识与法官流动——一种基于实证的分析 总被引:2,自引:0,他引:2
文章从一个有待检验的假设———最高法院可能愿意维持现有的法院行政性管理制度———入手,根据实地调研来的大量法官数据,展示了中国各级法院内部频繁的行政性流动的局部场景,并运用利益逻辑和成本———收益方法论证了法官为什么流动,法院和法官为什么忽视司法知识的积累等问题。更进一步地,借用激励理论、管理学和博弈论的工具,文中还论证了司法逻辑和行政逻辑之间的迥然差异,分析了中国法院系统忽视司法制度逻辑的原因以及改变这种行政性制度安排和行政性管理逻辑的困难和艰巨性。不仅验证了假设,还指出了法官的行政性流动制度和专业司法知识积累之间的张力,揭示了中国法院高度行政化管理现实背后的深层制度性成因。 相似文献
17.
目的利用上转换发光免疫层析技术(UPT-LF)建立一种尿液中吗啡(MOP)及甲基苯丙胺(MET)快速定量检测方法并对其进行系统评价。方法以上转换发光纳米颗粒(UCP-NPs)作为生物示踪物,竞争模式免疫层析作为检测平台,建立可对尿液中MOP及MET进行定量检测的UPT-LF,即MOP-UPT-LF、MET-UPT-LF。以MOP-UPT-LF为代表,评价UPT-LF对痕量毒品检测极限,通过系列浓度标准品测定,评价定量检测能力。根据常规检测阈值,调整MOP-UPT-LF及MET-UPT-LF检测敏感性及线性范围,并评价其定量检测能力。以LC-MS、GC-MS分别作为MOP、MET检测的金标准,以胶体金免疫层析作为对照,对执法现场收集尿样进行检测,确定UPT-LF定性检测性能。对系列浓度MOP模拟阳性样本同时进行LC-MS及MOP-UPT-LF定量检测,对系列浓度MET模拟阳性样本同时进行GC-MS及MET-UPT-LF定量检测,评价UPT-LF定量检测性能。结果在痕量检测条件下,MOP-UPT-LF敏感性可达1ng/m L,线性范围为1~5000ng/m L(r=-0.98172,P<0.0005)。在常规检测条件下,MOP-UPT-LF敏感性为50ng/m L,线性范围调整为50~3000ng/m L(r=-0.98464,P<0.0005);MET-UPT-LF敏感性为100ng/m L,线性范围为100~5000ng/m L(r=-0.99964,P<0.0005)。就定性检测而言,MOP-UPT-LF及MET-UPT-LF均较优,灵敏度及特异度均为100%,与胶体金结果一致。就定量检测而言MOP-UPT-LF及MET-UPT-LF与定量确证方法LC-MS及GC-MS无显著差异。结论本研究建立MOP-UPT-LF、MET-UPT-LF方法,在满足快筛试剂快速简便的基础上,进一步实现了现场快速定量检测,为尿液中毒品的现场快速定量检测提供了技术保障。 相似文献
18.
经过大量的社会调查,发现大多数学习书法的小学生是在众多的压力之下,同时又是在缺少愉快心理的过程中度过的。为什么欣赏起来赏心悦目的书法,学起来却令人痛苦不堪呢?这主要是小学生学习书法的心理偏差造成的,只有消除偏差,才能培养他们学习书法的积极性。 相似文献
19.
建立70%乙醇灌胃致大鼠胃粘膜急性损伤模型,以胃粘膜血流量,胃跨壁电位差,胃粘膜损伤指数为指标,并测定血液中,胃粘膜中一氧化氮含量,研究针灸对胃粘膜损伤的保护作用及其与NO的关系。结果显示:针灸对胃粘膜损伤具有保护作用。NO的前体L-精氨酸和NO的代价供体硝普钠对胃粘膜也有保护作用,而NO合成酶的抑制剂N-亚硝基左旋精氨酸未显示出胃粘膜有保护作用。 相似文献
20.
Thomas J. Scotto Harold D. Clarke Allan Kornberg Jason Reifler David Sanders Marianne C. Stewart Paul Whiteley 《Electoral Studies》2010,29(4):545-556
In recent years, students of voting behavior have become increasingly interested in valence politics models of electoral choice. These models share the core assumption that key issues in electoral politicds typically are ones upon which there is a widespread public consensus on the goals of public policy. The present paper uses latent curve modeling procedures and data from a six-wave national panel survey of the American electorate to investigate the dynamic effects of voters’ concerns with the worsening economy—a valence issue par excellence—in the skein of causal forces at work in the 2008 presidential election campaign. As the campaign developed, the economy became the dominant issue. Although the massively negative public reaction to increasingly perilous economic conditions was not the only factor at work in 2008, dynamic multivariate analyses show that mounting worries about the economy played an important role in fueling Barack Obama’s successful run for the presidency. 相似文献