人才分布及其流动的公共部门化现象探析——以云南省为个案的实证研究

本文是以云南省为个案,对人才分布及其流动的公共部门化现象的实证研究。人才作为第一资源,在市场经济条件下的配置,首要和主要的,应是面向经济、走向市场,进入经济实体。但目前我国情况并非如此,尤其是在欠发达地区,人才分布和流动的公共部门化是一种普遍的现象。这种现象如果不能有效改变,将对我国的经济发展与市场经济体制的巩固产生重大的消极影响。     

"智慧景区"背景下交通组织管理方式探究

"智慧景区"已经成为我国旅游业发展的新趋势,利用大数据可对景区运行状态进行整体把控,系统性地诊断景区管理问题成因,及时监测和发现景区问题区域,提出景区游览管理措施和方案。就景区外部而言,利用大数据可分析游客在景区外部游览路线趋势。在此基础上,组织者可通过对周边道路采取交通管控措施,实现对游客进出景区的合理诱导,缓解景区进出口通道的交通拥堵。就景区内部而言,利用大数据可分析各景点人流聚集与移动情况。同时,组织者可采取"容量限制"和"流向调控"等有效管理措施,实现对人流游览路径的调控,对游客进行科学的疏导和分流,提升景区管理服务水平。     

乳糜微粒流对黄芩苷纳米乳动物体内组织分布的影响研究

目的 探究乳糜微粒流对黄芩苷纳米乳大鼠体内淋巴组织的分布,初步阐明黄芩苷纳米乳具有淋巴靶向性的机制.方法 利用高效液相色谱法测定生物样品中黄芩苷浓度;大鼠分别灌胃给予黄芩苷纳米乳和黄芩苷混悬液,剂量为144.0 mg/kg;建立大鼠乳糜微粒流阻断模型,考察乳糜微粒介导的跨膜转运对黄芩苷纳米乳在淋巴组织器官分布的影响.结果 与未阻断组相比,大鼠体内乳糜微粒流的阻断,可影响黄芩苷纳米乳组大鼠体内脾、胸腺和淋巴结血药浓度-时间曲线下面积、药峰浓度等参数,但未对黄芩苷混悬剂组大鼠的体内药物代谢动力学参数产生影响.结论 黄芩苷纳米乳刺激乳糜微粒的分泌,增加药物经淋巴途径的转运量,药物富集于淋巴器官,可能是黄芩苷纳米乳靶向淋巴器官的主要机制.     

Application of fluorescence lifetime imaging (FLIM) in latent finger mark detection

Fluorescence lifetime imaging (FLIM) in frequency domain enables the mapping of the spatial distribution of fluorescence lifetimes of a specimen. It has been extensively applied in biology. In this paper, a theoretical analysis for the fluorescence lifetime determination of latent finger mark samples is described, which is followed by the feasibility study of using FLIM in frequency domain for latent finger marks detection. Preliminary experiments are carried out with latent finger marks treated with a fluorescent powder on two different substrates. The resulting fluorescence lifetime image of finger mark revealed a good contrast, and was able to detect the latent finger marks clearly.     

浅谈路由基础结构免遭拒绝服务

当网络遭受拒绝服务时，信息包不能达到目的地，已有的邮件路由协议不能很好地解决拒绝服务问题。作者在文中提出了路由基础结构拒绝服务的模型，并描述了用入侵检测保护路由基础结构免遭拒绝服务的方法。     

平交路口左转交通流的组织与管理

如何合理组织与控制路口的左转弯交通流,是平交路口交通秩序管理及提高路口通行能力的核心问题。可以通过连续向右绕行,远引交叉等措施实现左转。同时这种路口禁止左转弯的交通管制要与路网布局匹配,尽可能不给车辆的行驶造成太大的不便。在条件允许的地方,仍需通过合理渠化设置左转专用车道和相位。     

The effect of chlorine and hydrogen chloride on latent fingermark evidence

Exposure of forensic evidence to either hydrogen chloride or chlorine can result in acidification to such an extent that enhancement of fingermarks with ninhydrin or cyanoacrylate is inhibited. Under these circumstances, pretreatment of samples with volatile bases such as triethylamine or ethanolamine prior to using these enhancement techniques can lead to successful visualization of fingermarks. Alternatively, physical enhancement techniques such as powder dusting or small particle reagent can be used on acidified non-porous substrates, although the former technique is subject to increased background under such conditions.     

不同光照条件下褪黑素对鸡鸭鹌鹑外周血淋巴细胞及其亚群变化的影响

为探讨不同褪黑素水平对禽类机体免疫系统的调节作用,通过控制不同光照条件,分3组(短光照组、长光照组和对照组)饲养鸡45只、鸭45只、鹌鹑45只,采用荧光双色染色法和流式细胞仪分别检测鸡、鸭、鹌鹑外周血中CD3 、CD4 、CD8 T淋巴细胞及其亚群和Bu-1a B淋巴细胞数量变化。结果,短光照组由于体内褪黑素水平较高,外周血中CD3 CD4 T细胞、CD3 CD8 T细胞、Bu-1a B细胞及白细胞总数都有不同程度升高,且与对照组相比差异显著;长光照组由于体内褪黑素分泌较少,外周血中CD3 CD4 T细胞、CD3 CD8 T细胞、Bu-1a B细胞及白细胞总数都有不同程度降低,且与对照组相比差异显著。结果表明,内源性褪黑素对鸡、鸭、鹌鹑外周血中淋巴细胞及其亚群的数量变化有显著影响。     

Nitrate and Nitrite Determination in Gunshot Residue Samples by Capillary Electrophoresis in Acidic Run Buffer,

Simultaneous determination of nitrate and nitrite in gunshot residue has been conducted by capillary electrophoresis using an acidic run buffer (pH 3.5). In previously developed capillary electrophoretic methods, alkaline pH separation buffers were used where nitrite and nitrate possess similar electrophoretic mobility. In this study, the electroosmotic flow has been reversed by using low pH running buffer without any additives. As a result of reversing the electroosmotic flow, very fast analysis has been actualized, well‐defined and separated ion peaks emerge in less than 4 min. Besides, the limit of detection was improved by employing large volume sample stacking. Limit of detection values were 6.7 and 4.3 μM for nitrate and nitrite, respectively. In traditional procedure, mechanical agitation is employed for extraction, while in this work the extraction efficiency of ultrasound mixing for 30 min was found sufficient. The proposed method was successfully applied to authentic gunshot residue samples.     

Developmental Validation of RSID™-Saliva: A Lateral Flow Immunochromatographic Strip Test for the Forensic Detection of Saliva

Abstract:  Current methods for forensic identification of saliva generally assay for the enzymatic activity of α-amylase, an enzyme long associated with human saliva. Here, we describe the R apid S tain ID entification (RSID™-Saliva), a lateral flow immunochromatographic strip test that uses two antisalivary amylase monoclonal antibodies to detect the presence of salivary amylase, rather than the activity of the enzyme. We demonstrate that RSID™-Saliva is accurate, reproducible, and highly sensitive for human saliva; RSID™-Saliva detects less than 1 μL of saliva. The sensitivity of RSID^TM-Saliva allows investigators to sample a fraction of a questioned stain while retaining the majority for DNA-STR analysis. We demonstrate that RSID™-Saliva identifies saliva from a variety of materials (e.g., cans, bottles, envelopes, and cigarette-butts) and it does not cross-react with blood, semen, urine, or vaginal fluid. RSID™-Saliva is a useful forensic test for determining which evidentiary items contain saliva and thus may yield a DNA profile.